[Down-regulation of vascular endothelial growth factor in human retinal pigment epithelial cells by small hairpin loop RNA targeting hypoxia inducible factor-1 alpha under hypoxia condition].

Objective: To explore the effect of hypoxia inducible factor-1 alpha (HIF-1 alpha) gene on the expression of vascular endothelial growth factor (VEGF) in human retinal pigment epithelial (hRPE) cells under hypoxia conditions by using small hairpin loop RNA (shRNA) to silence HIF-1 alpha.

Methods: CoCl(2) (150 micromol/L) was used to simulate the hypoxia environment for hRPE cells. After choosing a target site of HIF-1 alpha mRNA, shRNA was designed and synthesized by this target site.

[Matrix metalloproteinases inhibitor doxycycline inhibits TGF-beta 1-induced RPE cell migration].

Objective: To investigate the effects of matrix metalloproteinases (MMP) inhibitor (Doxycycline) on retinal pigment epithelial (RPE) cell migration induced by transforming growth factor beta 1 (TGF-beta 1).

Methods: The third to sixth passage human RPE cells cultures were treated with TGF-beta 1 at different concentrations (0.01, 0.

[The change of the level of the vascular endothelial growth factor in aqueous humor of patients with neovascular glaucoma before and after anterior retinal cryotherapy].

Objective: To compare the change of the level of the vascular endothelial growth factor (VEGF) in aqueous humor of patients with neovascular glaucoma (NVG) before and after anterior retinal cryotherapy and investigate the effects of the fluctuation of VEGF on the neovascularization of iris.

Methods: 28 patients with neovascular glaucoma were undergone iris fluorescent angiography to identify the area and amount of new vessels before and after anterior retinal cryotherapy. The neovascularization of iris was observed from 7 to 14 days by iris fluorescent angiography to confirm the regression of new vessels in iris before trabeculectomy.

[The inhibitory effects of angiostatin on retinal neovascularization induced by oxygen].

Objective: To observe the inhibitory effects of angiostatin on microvascular endothelial cells of mouse retina and test the efficacy of native angiostatin in suppressing experimental retinal neovascularization induced by oxygen.

Methods: Angiostatin was purified with L-lysine Sepharose 4B from human plasma. The primary microvascular endothelial cells from rat retina were cultured.

[Posterior vitreous detachment induced by intravitreal injection of the combination of chondroitinase ABC and matrix metalloproteinase-3].

Objective: To investigate the occurrence of enzymatic induction of posterior vitreous detachment (PVD) by the combination of Chondroitinase ABC (CA) and matrix metalloproteinase-3 (MMP-3), so as to seek a noninvasive and effective pharmacologic approach to facilitate and eventually replace the present mechanical vitreous surgery.

Methods: Twenty-four pigmented rabbits were randomly assigned to two groups of twelve each, the experimental group was treated with CA (0.2 U) and MMP-3 (10 ng) combination, the control group was received equivalent dose of balanced salt solution (BSS).