Folate is an essential nutrient for nearly all organisms. While the physiological function and mechanism aspects of folate have been extensively and deeply investigated in Eukarya, related researches in Bacteria remains poorly understood. In this study, we focus on physiological functions of folate in Lactiplantibacillus plantarum by employing a combination of genetics, biochemistry and microscopy approaches.
View Article and Find Full Text PDFBackground: During surveys of hypogeous fungi in the Hengduan Mountains, south-western China, three specimens of the genus were discovered in Sichuan Province.
New Information: Morphological and molecular analyses revealed that the collections represent a new species, . This article describes the new species and discusses its relationship with the other two members of the genus.
Exciplex organic light-emitting diodes (OLEDs) are widely utilized for their high internal quantum efficiency. Although solution-processed devices offer the advantages of simple operation and low cost, few studies have reported on the preparation of exciplex OLED devices using solution methods. This paper presents exciplex OLEDs produced between m-MTDATA and Bphen, fabricated using a solution method and optimized by a polar solvent vapor annealing (PSVA) treatment.
View Article and Find Full Text PDFTo address immune escape, multi-specific CAR-T-cell strategies use natural ligands that specifically bind multiple receptors on malignant cells. In this context, we propose a split CAR design comprising a universal receptor expressed on T cells and ligand-based switch molecules, which preserves the natural trimeric structure of ligands like APRIL and BAFF. Following optimization of the hinges and switch labeling sites, the split-design CAR-T cells ensure the native conformation of ligands, facilitating the optimal formation of immune synapses between target cancer cells and CAR-T cells.
View Article and Find Full Text PDFFood Microbiol
January 2025
Thermomyces dupontii harbors two paralogs ( and ) in the gene cluster for the biosynthesis of prenylated indole alkaloids (PIAs) and correponding iron chelators with assigned as one protein containing a CYP like domain fused with a FAD-binding domain-containing oxidoreductase. Genetic manipulation and metabolic profile analysis indicated both and were involved in transforming simple PIAs to their corresponding iron chelators. Moreover, is responsible for bolstering simple PIAs to complex PIAs, and for reinforcing conjugating unsaturated systems in complex PIAs.
View Article and Find Full Text PDFTo date, there are no real physiological mechanisms for iron excretion in eukaryote, and no physiological "actuator" that can control all the three fundamental biologic processes of absorption, storage, and excretion. Here, we observed that the accumulation of anthraquinones by Thermomyces dupontii under cold stress can achieve this process. Through mutation analysis, we found that mutant ΔAn deficiency in anthraquinones accumulated ferrous and total free iron due to adopting a rare lifestyle with no endocytosis but accumulation of membrane-derived vesicles.
View Article and Find Full Text PDFTwo new species and are described and illustrated, based on morphological characteristics and molecular phylogenetic analysis. Two new records, and from Thailand, are also reported. is characterized by small basidiomata, stipe equal with an enlarged base, and nearly subclavate, pale pink to light orange.
View Article and Find Full Text PDFThree newly discovered species, namely , , and , are introduced and illustrated based on both morphological and molecular data from Sichuan and Yunnan provinces in China. A multigene phylogenetic analysis (nrITS, nrLSU, and ) was performed mainly to verify the placement of the new species in . A second, nrITS-only phylogenetic analysis comprising more species for which only ITS sequences were available, was used to infer the relationship between the new species and as many known species as possible.
View Article and Find Full Text PDFComp Biochem Physiol Part D Genomics Proteomics
December 2024
The Alexandrine parakeet (Palaeornis eupatria), also known as the Alexandrine parrot, is a critically endangered species in the world and a national second class protected animal. Current knowledge on gut microbiome and metabolome of captive Alexandrine parrots is limited. In the current study, we characterized the effect of dietary change with pellet feeding on the gut microbiome and metaboliome in Alexandrine parrots using 16S gene sequencing and liquid chromatography with tandem mass spectrometry (LC-MS/MS).
View Article and Find Full Text PDFThree new species of , , , and belonging to the phylogroup, are described based on specimens collected in alpine forests at 3600-4000 m, Northwest Yunnan, China. is distinguished by its ascomata with a single chamber of 0.5-1.
View Article and Find Full Text PDFOrganic ultralong room temperature phosphorescence (OURTP) materials capable of combining various emission behaviors for diversified optoelectronic properties and applications have recently gained a vigorous development, but it remains a forbidden challenge in designing OURTP molecules with hybrid local and charge-transfer (HLCT) feature, possibly due to the elevated difficulties in simultaneously meeting the stringent requirements of both HLCT and OURTP emitters. Here, through introducing multiple heteroatoms into one-dimensional fused ring of coumarin with moderate charge transfer perturbation in donor-π-acceptor architecture, we demonstrate a HLCT-featured OURTP molecule showing both promoted fluorescence with a quantum yield of 77 % in solution and long-lived OURTP with a lifetime of 251 ms in conventional host material used in electroluminescent device. Thus, efficient OURTP organic light-emitting diodes (OLEDs) were fabricated, exhibiting bright electroluminescence with an exciton utilization efficiency of 85 % and yellow OURTP lasting over 2 s for afterglow.
View Article and Find Full Text PDFis a large and diverse genus containing fungi that cause white rot to infect a number of plant families. This study describes and as new species from Southwest China, based on morphological and molecular evidence. is characterized by dark brown to purplish black pileus surface with dense concentric furrows, pale yellow margin, irregular pileipellis cells, small pores (5-7 per mm) and ellipsoid to sub-globose basidiospores (8.
View Article and Find Full Text PDFThe adsorption characteristics of β-glucooligosaccharides on activated carbon and the purification were systematically investigated. The maximum adsorption capacity of activated carbon reached 0.419 g/g in the optimal conditions.
View Article and Find Full Text PDFThe interaction between the intrinsic polarity of the host material and the TADF guest material affects charge injection and transport, exciton formation, charge recombination, and emission mechanisms. Therefore, understanding and controlling the interaction between the intrinsic polarity of the host material and the TADF guest material is very important to realize efficient TADF-OLED devices. This study investigated the molecular interaction between different polar host materials and a thermally activated delayed fluorescence material (DMAc-PPM).
View Article and Find Full Text PDFSpinal cord injury (SCI), caused by significant physical trauma, as well as other pathological conditions, results in electrical signaling disruption and loss of bodily functional control below the injury site. Conductive biomaterials have been considered a promising approach for treating SCI, owing to their ability to restore electrical connections between intact spinal cord portions across the injury site. In this study, we evaluated the ability of a conductive hydrogel, poly-3-amino-4-methoxybenzoic acid-gelatin (PAMB-G), to restore electrical signaling and improve neuronal regeneration in a rat SCI model generated using the compression clip method.
View Article and Find Full Text PDFThe "instant" quality of instant rice noodles is significantly affected by slow rehydration during cooking. This happens as a result of the native rice starch's low ability to gelatinize as well as the high shear and pressure utilized in industries during the widely used extrusion molding process. In order to address this issue, the rice flour was pretreated with mild steam (MS) technology.
View Article and Find Full Text PDFThe formation of the trapping device induced by nematodes has been assumed as an indicator for a switch from saprophytic to predacious lifestyles for nematode-trapping fungi. However, fungal nematocidal activity is not completely synonymous with fungal trap formation. We found that the predominant nematode-trapping fungus harbored a rare () gene cluster that was mainly distributed in nematode-trapping fungi.
View Article and Find Full Text PDFFlexible organic light-emitting diodes (FOLEDs) have promising potential for future wearable applications because of their exceptional mechanical flexibility. Silver nanowire (Ag NW) networks are the most promising candidates to replace indium tin oxide (ITO), which is limited by its poor bendability. In this study, three different methods including methanol impregnation, argon plasma treatment, and ultraviolet radiation were used to reduce the junction resistance of Ag NWs to optimize the flexible transparent electrodes (FTEs); which were prepared using Ag NWs and poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT: PSS).
View Article and Find Full Text PDFIt has been documented that the uterus plays a key cardio-protective role in pre-menopausal women, which is supported by uterine cell therapy, to preserve cardiac functioning post-myocardial infarction, being effective among females. However, whether such therapies would also be beneficial among males is still largely unknown. In this study, we aimed to fill in this gap in knowledge by examining the effects of transplanted uterine cells on infarcted male hearts.
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