Runjing extract promotes spermatogenesis in rats with ornidazole-induced oligoasthenoteratozoospermia through extracellular signal-regulated kinase signalling, and regulating vimentin expression.

Objective: To investigate the efficacy of Runjing (RJ) extract on oligoasthenoteratozoospermia (OAT) induced by ornidazole (ORN) in rats, and to study the underlying mechanism.

Methods: Twenty-four adult male Sprague-Dawley rats were treated with normal saline (control), ORN (OAT model), ORN + 4.725 g·kg－1·d－1 RJ extract (low-dose) and ORN+ 18.

Significance of TP53 mutation in bladder cancer disease progression and drug selection.

Background: The tumor protein p53 (TP53) mutant is one of the most frequent mutant genes in bladder cancer. In this study, we assessed the importance of the TP53 mutation in bladder cancer progression and drug selection, and identified potential pathways and core genes associated with the underlying mechanisms.

Methods: Gene expression data used in this study were downloaded from The Cancer Genome Atlas and cBioportal databases.

Identification of key pathways and genes in PTEN mutation prostate cancer by bioinformatics analysis.

Background: Prostate cancer (Pca) remains one of the leading adult malignancies. PTEN (Phosphatase and Tensin Homolog) mutant is the top common mutated genes in prostate cancer, which makes it a promising biomarker in future individualized treatment.

Methods: We obtained gene expression data of prostate cancer from TCGA (The Cancer Genome Atlas) database for analysis.

Significance of PTEN Mutation in Cellular Process, Prognosis, and Drug Selection in Clear Cell Renal Cell Carcinoma.

It is well established that the PTEN (Phosphatase and Tensin Homolog) mutant is a frequently mutated gene found in clear cell renal cell carcinoma (ccRCC), making it a potential biomarker for individualized treatment opinions. Here, in the present study, we designed a method to evaluate the significance of the PTEN mutation in the prognosis and drug selection of ccRCC, determine the potential changing pathways and genes associated with the mechanisms. The most recent TCGA data shows that the PTEN mutation is found in 5% of ccRCC patients.

EZH2 Expression is increased in BAP1-mutant renal clear cell carcinoma and is related to poor prognosis.

BAP1 is frequently mutated in clear cell renal cell carcinoma (ccRCC) with a definitive role still unclear. In silico analysis of BAP1-mutant and wild-type gene enrichment and functional annotation in TCGA-KIRC dataset was performed. Target gene was studied based on functional clustering and was knowledge-based.

[Diagnosis and treatment of slow transit constipation complicated with adult megacolon].

Objective: To summarize the experience in the management of slow transit constipation complicated with adult megacolon.

Methods: The clinical data of 32 above patients admitted between October 2007 and June 2011 were retrospectively studied.

Results: Thirty-two patients were diagnosed as slow transit constipation according to the Roman III criteria.