Publications by authors named "Shue Chen"
Article Synopsis
- Mitochondria-ER contact sites (MERCs) are crucial for regulating mitochondrial functions, facilitated by proteins like REEP5 that shape the ER tubules.
- REEP5 interacts with Mitofusins 1/2 to promote mitochondrial distribution in the cytoplasm through a novel "hitchhiking" transport mechanism along microtubules.
- Changes in REEP5 levels affect how mitochondria are tethered and distributed, with implications for the production of reactive oxygen species (ROS), which are essential for various physiological processes.
Article Synopsis
- Chromatin insulators are important DNA-protein complexes that regulate gene expression by preventing repressive chromatin spread and blocking interactions between enhancers and promoters.
- In Drosophila, the gypsy insulator complex is made up of three key proteins (CP190, Su(Hw), and Mod(mdg4)67.2) that concentrate in structures called insulator bodies, and their positioning is influenced by the nucleosome remodeling factor NURF301/E(bx).
- NURF301 enhances the function of the gypsy insulator by promoting interactions between insulator proteins, repositioning nucleosomes, and facilitating the spatial organization of insulator bodies within the nucleus.
Motivation: ChIP-seq detects protein-DNA interactions within chromatin, such as that of chromatin structural components and transcription machinery. ChIP-seq profiles are often noisy and variable across replicates, posing a challenge to the development of effective algorithms to accurately detect differential peaks. Methods have recently been designed for this purpose but sometimes yield conflicting results that are inconsistent with the underlying biology.
View Article and Find Full Text PDFGenome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization.
View Article and Find Full Text PDFClasses of retrotransposons constitute a large portion of metazoan genome. There have been cases reported that genomic abundance of retrotransposons is correlated with the severity of low environmental temperatures. However, the molecular mechanisms underlying such correlation are unknown.
View Article and Find Full Text PDFBackground: MicroRNAs (miRNAs) play important roles in regulating the expression of protein-coding genes by directing the degradation and/or repression of the translation of gene transcripts. Growing evidence shows that miRNAs are indispensable player in organismal development with its regulatory role in the growth and differentiation of cell lineages. However, the roles of miRNA-mediated regulation in environmental adaptation of organisms are largely unknown.
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