Different mutations at position 562 of the hepatitis E virus capsid proteins exhibit differential effects on viral neutralizing activity.

The hepatitis E virus (HEV) capsid protein pORF2 comprises three potential N-linked glycosylation sites. One site, N562, is located at the cell attachment and neutralizing antigenic regions. The present study performed detailed analyses of the effects of specific amino acid substitutions at position 562 in the homodimerization, glycosylation, antigenicity, immunogenicity and neutralization activities of HEV pORF2.

The efficacy and safety of entecavir in patients with chronic hepatitis B- associated liver failure: a meta-analysis.

Background: The effectiveness of nucleoside analogue (NA) treatment in patients with chronic hepatitis B (CHB) -associated liver failure is still controversial. Severe lactic acidosis has been reported during entecavir (ETV) treatment in patients with impaired liver function.

Aim: To investigate the rescuing efficacy and safety of ETV in patients with CHB-associated liver failure.

Intracellular levels of hepatitis B virus DNA and mutational patterns of the polymerase gene of hepatitis B virus in peripheral blood mononuclear cells of patients with lamivudine or telbivudine resistance.

Studies are limited regarding the association between the quantity of hepatitis virus B (HBV) DNA loads in serum and peripheral blood mononuclear cells (PBMCs) in patients with drug resistance and few studies focus on the mutational pattern of the polymerase gene of HBV in PBMCs of patients with drug resistance. The aim of the present study was to investigate the association between the quantity of HBV DNA loads in serum and PBMCs in patients with lamivudine (LAM) or telbivudine (LdT) resistance and to explore the mutational pattern of the polymerase gene of HBV in PBMCs of these patients. A total of 51 patients underwent antiviral therapy with LAM or LdT for at least half a year.

[Study of blood compatibility on TiO2 coated biomedical Ni-Ti shape memory alloy].

We coated a thin TiO2 film on the surface of Ni-Ti shape memory alloy by activated sputter method in the present work. The blood platelet adherence and antithrombogenicity of the TiO2-coated Ni-Ti alloy were evaluated. The results showed that the platelets on the TiO2-coated Ni-Ti alloy were fewer than those on 316L stainless steel, and no agglomeration or distortion for the platelets on the coated alloy was found, which means less probability of blood coagulation for the alloy.

[Preparation of anodic oxidation layer on the surface of pure titanium and its biological activity study].

This paper introduces how TiO2 film was prepared on pure titanium by anodic oxidation. Surface morphology and composition of the oxide film were analyzed by SEM coupled with EDAX. The deposition ability of hydroxyapatite of the anodized titanium in simulated body fluid (SBF) at 37 degrees C was evaluated.