The GP2a 91/97/98 amino acid substitutions play critical roles in determining PRRSV tropism and infectivity but do not affect immune responses.

Unlabelled: Porcine reproductive and respiratory syndrome virus (PRRSV) isolates share a restricted cellular tropism. Marc-145 cells derived from African green monkey are one of the few cell lines supporting PRRSV propagation and are commonly used for PRRS vaccine development. However, currently prevalent PRRSV isolates display different Marc-145 cell tropism while the exact determinant is not clarified yet.

High-Throughput Programmable Tumor Spheroid Generation Using the Magneto-Archimedes Effect.

Three-dimensional (3D) tumor spheroids in vitro have great potential in drug discovery and tissue engineering due to their similarity to real tissues. Herein, we develop a simple strategy, with time and cost-effective, noninvasive characters to produce high-throughput 3D tissue spheroids using the magneto-Archimedes effect. This method is versatile in producing various morphologies of tumor spheroids by using different templates, as well as spatially coded tumor spheroids by adding different cells in the defined orders.

Emergence, prevalence and evolution of porcine reproductive and respiratory syndrome virus 1 in China from 1994 to 2024.

Porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) was first detected in Chinese swine herds during an epidemiological investigation since 1994. Even though PRRSV-1 has been existed in China for 30 years, much less attention was paid on PRRSV-1 than PRRSV-2. This review systematically evaluated the emergence, prevalence and evolution of Chinese PRRSV-1 from 1994 to 2024.

Elucidating Local Confinement in Crowded Polymer Solutions Within Giant Unilamellar Vesicles (GUVs) Through Single Particle Tracking Toward Deeper Understanding of Cells.

In a crowded environment, macromolecules occupy a significant proportion volume of cells to repulse other molecules in HO-rich phase domains. These HO-rich phase domains have been found to significantly influence material transportation and biochemical reactions. However, the accurate quantification of the size of these domains remains a challenge.

Light-Driven Carbon Fixation Using Photosynthetic Organelles in Artificial Photosynthetic Cells.

Building an artificial photosynthetic cell from scratch helps to understand the working mechanisms of chloroplasts. It is a challenge to achieve carbon fixation triggered by photosynthetic organelles in an artificial cell. ATP synthase and photosystem II (PSII) are purified and reconstituted onto the phospholipid membrane to fabricate photosynthetic organelles.

Neural correlates of olfactory working memory in the human brain.

Previous research has revealed that the insula, pallidum, thalamus, hippocampus, middle frontal gyrus, and supplementary motor area are activated during odor memory and that the performance of olfactory working memory is affected by the verbalization of odors. However, the neural mechanisms underlying olfactory working memory and the role of verbalization in olfactory working memory are not fully understood. Twenty-nine participants were enrolled in a study to complete olfactory and visual n-back tasks using high- and low-verbalizability stimuli while undergoing fMRI imaging.

A novel olfactory sorting task.

Background: This study aimed to develop a simple self-administered screening tool for odor memory, which allowed users to self-test their olfactory function repeatedly even at home.

Methods: One hundred and ten participants were recruited (30 men, age = 50.1 ± 9.

Protective Effects of Lactoferrin Treatment Against Sodium Arsenite Exposure-Induced Nephrotoxicity.

It is said that a wide range of renal functions are at risk from arsenic exposure. We examined how lactoferrin administration may mitigate inflammation, apoptosis, redox imbalance, and fibrosis in order to counteract arsenic-induced nephrotoxicity. Accordingly, male C57BL/6 mice (6 weeks) were divided into six experimental groups with six mice in each group.

Effect of Gentianella acuta (Michx.) Hulten against the arsenic-induced development hindrance of mouse oocytes.

The current study was designed to investigate the alleviative effect of Gentianella acuta (Michx.) Hulten (G. acuta) against the sodium arsenite (NaAsO)-induced development hindrance of mouse oocytes.

A chimeric porcine reproductive and respiratory syndrome virus 1 strain containing synthetic ORF2-6 genes can trigger T follicular helper cell and heterologous neutralizing antibody responses and confer enhanced cross-protection.

The prevalence of porcine reproductive and respiratory syndrome virus 1 (PRRSV1) isolates has continued to increase in Chinese swine herds in recent years. However, no effective control strategy is available for PRRSV1 infection in China. In this study, we generated the first infectious cDNA clone (rHLJB1) of a Chinese PRRSV1 isolate and subsequently used it as a backbone to construct an ORF2-6 chimeric virus (ORF2-6-CON).

Xylem plasticity of root, stem, and branch in under drought stress: implications for whole-plant hydraulic integrity.

Introduction: A better understanding of xylem hydraulic characteristics in trees is critical to elucidate the mechanisms of forest decline and tree mortality from water deficit. As well as temperate forests and forests growing in arid regions, subtropical and tropical forests are also predicted to experience an increased frequency and intensity of climate change-induced drought in the near future.

Methods: In this study, 1-year-old seedlings (a typical subtropical species in southern China) were selected for a continuous controlled drought pot experiment of 45 days duration.

Oscillation of type IV pili regulated by the circadian clock in cyanobacterium PCC7942.

Type IV pili (TFP) are known to be functionally related to cell motilities and natural transformation in many bacteria. However, the molecular and ecological functions of the TFP have rarely been reported for photosynthetic cyanobacteria. Here, by labeling pili in model cyanobacterium PCC 7942 (Syn7942), we have quantitatively characterized the TFP and its driven twitching motility in situ at the single-cell level.

Regulation of species metabolism in synthetic community systems by environmental pH oscillations.

Constructing a synthetic community system helps scientist understand the complex interactions among species in a community and its environment. Herein, a two-species community is constructed with species A (artificial cells encapsulating pH-responsive molecules and sucrose) and species B (Saccharomyces cerevisiae), which causes the environment to exhibit pH oscillation behaviour due to the generation and dissipation of CO. In addition, a three-species community is constructed with species A' (artificial cells containing sucrose and G6P), species B, and species C (artificial cells containing NAD and G6PDH).

Genetically encoded gelation redox-responsive collagen-like protein hydrogel for accelerating diabetic wound healing.

Genetically encoded collagen-like protein-based hydrogels have demonstrated remarkable efficacy in promoting the healing process in diabetic patients. However, the current methods for preparing these hydrogels pose significant challenges due to harsh reaction conditions and the reliance on chemical crosslinkers. In this study, we present a genetically encoded approach that allows for the creation of protein hydrogels without the need for chemical additives.

The Dunning-Kruger effect: subjective health perceptions on smoking behavior among older Chinese adults.

Background: The intrinsic damage and external hazards of smoking are major risk factors for poorer health and are recognized as a global health issue of concern in geriatric health. This study aims to assess the Dunning-Kruger effect through the influence of subjective health perceptions on smoking behavior in older adults.

Methods: This study used data from the 2018 Chinese Longitudinal Healthy Longevity Survey (N = 9,683) provided by the Center for Healthy Aging and Development Studies at Peking University.

Efficacy of a porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) natural recombinant against a heterologous PRRSV-1 isolate both clustered within the subgroup of BJEU06-1-like isolates.

Porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) has been prevalent in more than 20 provinces of China. However, no PRRSV-1-specific vaccine is commercially available in China. To evaluate the feasibility of using a low virulent PRRSV-1 isolate against potential outbreaks caused by virulent Chinese PRRSV-1 isolates, here we evaluated the efficacy of a low virulent PRRSV-1 HLJB1 strain isolated in 2014 as live vaccine against a virulent PRRSV-1 SD1291 strain isolated in 2022.

Effects of Drought Stress on Non-Structural Carbohydrates in Different Organs of .

The Chinese fir (Lamb.) Hook. is an important timber conifer species in China.

Important Roles and Formation of Atmospheric Organosulfates in Marine Organic Aerosols: Influence of Phytoplankton Emissions and Anthropogenic Pollutants.

Organosulfates (OSs) could be potentially important compounds in marine organic aerosols, while their formation in marine atmospheres is far from clear due to a lack of cruise observations. In this work, shipboard atmospheric observations were conducted over the Yellow Sea and Bohai Sea to investigate the abundance and formation of biogenic isoprene/monoterpene-OSs in marine aerosols. The quantified OSs and NOSs accounted for 0.