[AntI-inflammatory, antipyretic and antimicrobial activity of flavonoid-containing extract of Gratiola officinalis L].

The extract of Gratiola officinalis L. has been obtained by an original method ensuring the maximum yield of flavonoids. The extract simultaneously exhibits high anti-inflammatory activity, selective antimicrobial properties (with respect to Staphylococcus aureus and Pseudomonas aeruginosa, but not to conditionally pathogenic E.

[Influence of electromagnetic emission at the frequencies of molecular absorption and emission spectra of oxygen and nitrogen oxide on the adhesion and formation of Pseudomonas aeruginosa biofilm].

Aim: Evaluate the influence of electromagnetic emission (EME) at the frequencies of molecular absorption and emission spectra of atmospheric oxygen and nitrogen oxide (MAES 02 and MAES NO respectively) on the adhesion, population progress and biofilm formation of Pseudomonas aeruginosa.

Materials And Methods: Adhesive activity was evaluated by mean adhesion index (MAI) of bacteria on human erythrocytes. Population growth dynamic was assessed by optical density index of broth cultures; biofilm formation--by values of optical density of the cells attached to the surface of polystyrol wells.

[Circulation of methicillin-resistant staphylococci in hospitals with different profiles].

Analysis of prevalence of methicillin-resistant staphylococci in hospitals with different specializations was performed. 2584 strains were isolated. Methicillin-resistant (MR) strains were present in different profile hospitals and their total prevalence between isolated strains was 12.

[Chemotherapeutic effect of 3-(5-nitrofuryl)-7-(5-nitrofurfurylidene)-3,3a,4,5,6,7-hexahydro-2H-indazole in experimental staphylococcal infection].

Chemotherapeutic activity of 3-(5-nitrofuryl)-7-(5-nitrofurfuryliden)-3, 3a, 4, 5, 6, 7-hexahydro-2H-indazol (compound 26) was studied on albino mice with experimental staphylococcal infection. The animals were contaminated intraperitoneally. The results of culture of the specimens of the organs of the mice killed within various terms and the values of the spleen bacterial index served as the criteria of the effectiveness.

[Donor activity of methicillin resistant staphylococci in transduction experiments].

Antibiotic resistance gene transmission from methicillin resistant strains of Staphylococcus aureus (MRSA), isolated in a burn care unit, was studied in transduction experiments with type phages 29, 52, 52A and in experiments with prophage induction. The results of the experiments demonstrated high donor activity of MRSA. Recombinants with different antibiotic resistance phenotypes were revealed, but there were no methicillin resistant staphylococci among them.

[Antimicrobial activity of new original 2-aryliden-6-furfuryliden cyclohexanones and hexahydroindazoles on their basis].

Antimicrobial activity of 25 new original 2-aryliden-6-furfuryliden cyclohexanones and hexahydroindazoles based on them was studied. The majority of the compounds had low or moderate activity against the test cultures of Staphylococcus spp., Pseudomonas aeruginosa, Proteus spp.

[Storage of industrial microorganisms entrapped into polymer matrices].

Our study of the techniques of long-term storage of the biomass of various strains of microorganisms, which cause breakdown or transformation of synthetic organic compounds, demonstrates that desiccated agar beads with immobilized microbial cells can be used for this purpose. In addition, the cells can be stored in desiccated matrices of agar or polyvinyl alcohol, coating synthetic cords. Such dry biocatalysts may be used for quick starting of bioreactors and in other biotechnological processes.

[Protective effects of agar during immobilization of strains capable to degrade aromatic compounds].

A study of utilization of toxic monoaromatic compounds by microbial cells incorporated into agar matrix revealed positive effects of immobilization on the rate and completeness of biodegradation. This increase in the degrading activity is probably due to a strong protective effect of the polysaccharide carrier. The protective properties of agar are not due to sorptional and diffusional processes in the matrix.

[The use of the television microscopicanalysis for the serodiagnosis of salmonellosis and brucellosis].

The possibility of using TV microscopic analysis for the laboratory diagnosis of salmonellosis and brucellosis is considered. The comparative analysis of this method and standard serodiagnostic methods was made in the study of sera taken from 134 salmonellosis patients 69 chronic brucellosis patients. The study demonstrated the advantages of the method of MIA (rapidity, high sensitivity, specificity), making it possible to recommend its wide use for the laboratory diagnosis of salmonellosis and brucellosis.

[A microbiological validation and criteria for the efficacy of the use of CO2 and YAG lasers in the surgical treatment of cholecystitis].

A considerable frequency of infected bed of the gallbladder in different forms of cholecystitis was established, the anaerobic microflora being inoculated more often. A direct dependence of the frequency of infecting the bile and gallbladder bed on the patient's age and duration of having chronic cholecystitis was shown. The sterilization and prevention of reinfection of the gallbladder bed after the removal of it can be achieved by the laser irradiation of the total bed surface inspite of the absence of clear symptoms of acute inflammation of the bladder and the surrounding tissues, and the cleansing should be performed after the manipulations on extrahepatic bile ducts are completed.

[Effects of a new derivative of 5-alkyl-3N-furanones on the colonization resistance of the intestine in albino mice].

By its antagonistic function normal microflora provides the intestine with resistance to colonization with exogenic opportunistic and pathogenic microorganisms. The drug was effective in inducing a decrease in the intestine colonization resistance which in its turn leads to filling of free ecological niches with exogenic microflora. In this connection the suggestion that specification of a new chemical agent should include along with other criteria its effect on colonization resistance is valid.

[Chemotherapeutic effectiveness of a new derivative of 5-alkyl-3N-furanones in experimental staphylococcal infection].

High chemotherapeutic efficacy of the compound 1929, a new derivative of 5-alkyl-3H-furanones was shown in albino mice with experimental staphylococcal infection caused by intraperitoneal administration to the animals. The efficacy was found to be higher than that of furagin used for comparison. The average therapeutic dose (AD50) of the compound for intraperitoneal administration amounted to 40 mg/kg while the average lethal dose (LD50) was 3000 mg/kg.

[The possible use of PKZh-type devices (liquid purity control devices) in the analysis of cell suspensions].

PKZh means "device for liquid purity control". A possibility is considered to use the native PKZh type device for carrying out quantitative analyses of cellular suspension components, for routine bacterial suspension, agglutinated bacterial suspension and erythrocyte suspension. The flowing photometric principle of particle recording, used in the device, allows to analyse biological suspensions with small amounts of components.

[Acinetobacter calcoaceticus strain with a wide spectrum of utilizing aromatic compounds and carrying a plasmid for resorcin degradation].

A bacterial strain was isolated from soil and identified as Acinetobacter calcoaceticus var. lwoffii. The strain can utilize a wide spectrum of aromatic compounds.

[Antibiotic sensitivity of opportunistic bacteria isolated from patients].

Sensitivity of 147 Enterobacteria strains isolated from feces of various patients was determined with the method of serial dilutions on solid nutrient media. 8 antibiotics were tested. By genera (species) the microorganisms were arranged in the following order: E.

Effect of antibiotics on fatty acid composition of strains of E. coli K12 carrying R-plasmids.

A dynamic approach was employed for a study of the reaction of the fatty acid pool of sensitive and resistant strains of E. coli K12 in response to the introduction of antibiotics. Bacteria whose resistance is controlled by plasmids exhibited a specific reaction - a reversible increase in the concentration of cis-9,10-methyl-enehexadecanoic acid.