Long non-coding RNA MEG3 regulates the progress of osteoarthritis by regulating the miR-34a/Klotho axis.

Background: Osteoarthritis (OA) is one of the most common chronic diseases today, and its prevalence and incidence are expected to increase as life expectancy increases. By investigating the inhibition of long non-coding RNA maternally expressed gene 3 (lncRNA MEG3) in OA, we hope to provide some new insights into the treatment of osteoarthritis.

Methods: By constructing an osteoarthritis model, the knee joint tissue of the model was observed using hematoxylin and eosin staining (HE) and computed tomography (CT).

LncRNA MEG3 promotes osteogenesis of hBMSCs by regulating miR-21-5p / SOD3 axis.

Background: This study aimed to investigate the role of long non-coding (Lnc) RNA MEG3 on the osteogenesis of human bone marrow mesenchymal stem cells (hBMSCs).

Materials And Methods: The binding of miR-21-5p to LncRNA MEG3 and SOD3 was determined using luciferase reporter assay; fluorescence quantitative PCR was used to detect the expression of LncRNA MEG3 at different induction times. hBMSCs were transfected with LncRNA MEG3 overexpression vector and induced for osteoblasts for 14 days.

Bandwidth-tunable THz absorber based on diagonally distributed double-sized VO disks.

Terahertz absorbers combined with phase-changing are a class of stealth materials with adjustable absorptance. However, such absorbers still suffer from insufficient absorption bandwidth. We propose a three-layer terahertz (THz) absorber, consisting of an array of diagonally distributed double-sized disks on a silica-coated gold film.

Potential In Vitro Tissue-Engineered Anterior Cruciate Ligament by Copolymerization of Polyvinyl Alcohol and Collagen.

Background And Purpose: Suitable tissue-engineered scaffolds to replace human anterior cruciate ligament (ACL) are well developed clinically as the development of tissue engineering. As water-soluble polymer compound, polyvinyl alcohol (PVA) has been wildly used as the materials to replace ACL. The aim of this study was to explore the feasibility of constructing tissue-engineered ACL by the copolymerization of PVA and collagen (PVA/COL).

Bcl-xL expression following articular cartilage injury and its effects on the biological function of chondrocytes.

This study aimed to investigate the expression of B-cell lymphoma-extra large (Bcl-xL) in cartilage tissues following articular cartilage injury and to determine its effects on the biological function of chondrocytes. A total of 25 necrotic cartilage tissue samples and 25 normal tissue samples were collected from patients diagnosed with osteoarthritis at our hospital from December 2015 to December 2018. The mRNA expression levels of Bcl-xL, caspase-3, and matrix metalloproteinase-3 (MMP-3) in the normal and necrotic tissues were examined via quantitative polymerase chain reaction, and their protein expression levels were detected via western blotting.

The reassuring CT scan with a lethal diagnosis.

We discuss an interesting case of a patient who presented with symptoms of abdominal and worsening chronic back pain with a known history of abdominal aortic aneurysm.

DNA-functionalized gold nanoparticle-based fluorescence polarization for the sensitive detection of silver ions.

Despite their practical applications, Ag ions are environmental pollutants and affect human health. So the effective detection methods of Ag ions are imperative. Herein, we developed a simple, sensitive, selective, and cost-effective fluorescence polarization sensor for Ag detection in aqueous solution using thiol-DNA-functionalized gold nanoparticles (AuNPs).

Bcl-xL expression improves the therapeutic effect of human umbilical cord stem cell transplantation on articular cartilage injury in rabbit.

Background: To investigate the therapeutic effect of transplantation of B-cell lymphoma-extra large (Bcl-xL) gene modified human umbilical cord blood stem cells (HUCSCs) on rabbit articular cartilage injury.

Materials And Methods: HUCSCs were isolated and identified. Lentiviral encoding Bcl-xL was applied to modify HUCSCs.

Exploring the interaction of silver nanoparticles with lysozyme: Binding behaviors and kinetics.

The role of nanoparticle interaction with biomolecules to form a biocorona is the key to nanoparticle behavior and its consequences in the physiological environment. Since the adsorbed biocorona decides the fate of a nanomaterials in vivo, and thus a comprehensive understanding of the dynamic interactions of the proteins with the nanoparticle is imperative. Herein we investigate the interaction of a model protein, lysozyme with silver nanoparticles (AgNPs) using fluorescence, synchronous fluorescence, UV-vis absorption spectrum and circular dichroism (CD) techniques under the physiological conditions.

Inhibition of microRNA-21 decreases the invasiveness of fibroblast-like synoviocytes in rheumatoid arthritis via TGFβ/Smads signaling pathway.

Objectives: MicroRNA-21 (miR21) is aberrantly elevated in rheumatoid arthritis (RA) patients, the significance of this microRNA in RA pathogenesis and treatment, however, has not been investigated. In this study, by using RA-derived fibroblast-like synoviocyte (FLS) cells as a model, we investigated the effect and corresponding mechanism of miR21 inhibition on FLSs invasion.

Materials And Methods: miR21 expression in synovial tissue and FLSs in RA patients and non-RA controls were determined by stem-loop RT-PCR.

Modified Blair ankle fusion for ankle arthritis.

Objective: To investigate the clinical outcome of modified Blair ankle fusion for ankle arthritis.

Methods: Between November 2009 and June 2012, 28 patients with ankle arthritis were treated, among whom 11 had obvious foot varus deformity, and 17 were almost normal in appearance. There were 13 males and 15 females with an average age of 49.

[The methods of shorting proximal femoral and total hip arthroplasty for Crowe IV dysplastic hip of adults].

Objectives: To discuss the methods and outcome of shorting proximal femoral and total hip arthroplasty for Crowe IV dysplastic hip of adults.

Methods: From July 2000 to February 2006, 13 cases of osteoarthritis secondary to severe development dysplastic hip were treated by total hip replacement and the shorting proximal femoral.

Results: The duration of follow-up ranged from 4 months to 55 months.