Evaluation of penicillin-resistance and probiotic traits in Lactobacillus plantarum during laboratory evolution.

The aim of this study was to obtain the Lactobacillus plantarum ATCC14917 with high-level resistance to penicillin and evaluate their probiotic traits using laboratory evolution assay and whole-genome sequencing. In penicillin environment, the minimum inhibitory concentration (MIC) of L. plantarum to penicillin increased from 1 μg/mL to 16 μg/mL and remained stable after the removal of antibiotic pressure, suggesting that the resistance acquisition to penicillin was an irreversible process.

Attention-based cross domain graph neural network for prediction of drug-drug interactions.

Drug-drug interactions (DDI) may lead to adverse reactions in human body and accurate prediction of DDI can mitigate the medical risk. Currently, most of computer-aided DDI prediction methods construct models based on drug-associated features or DDI network, ignoring the potential information contained in drug-related biological entities such as targets and genes. Besides, existing DDI network-based models could not make effective predictions for drugs without any known DDI records.

Total Flavonoids of Drynariae Rhizoma Prevent Bone Loss Induced by Hindlimb Unloading in Rats.

Drynariae Rhizoma is a kidney-tonifying herb that has a long history in clinical practice for the treatment of bone fractures and joint diseases in China. Flavonoids are considered to be its major active ingredients and are reported to ease bone loss in ovariectomized rats. However, the beneficial effects of the total flavonoids of Drynariae Rhizoma on osteoporosis caused by microgravity or mechanical inactivity remain unknown.

Effects of total flavonoids from Drynariae Rhizoma prevent bone loss in vivo and in vitro.

Estrogen deficiency is one of the major causes of osteoporosis in postmenopausal women. Drynariae Rhizoma is a widely used traditional Chinese medicine for the treatment of bone diseases. In this study, we investigated the therapeutic effects of the total Drynariae Rhizoma flavonoids (DRTF) on estrogen deficiency-induced bone loss using an ovariectomized rat model and osteoblast-like MC3T3-E1 cells.

Combination of transcriptomic and metabolomic analyses reveals a JAZ repressor in the jasmonate signaling pathway of Salvia miltiorrhiza.

Jasmonates (JAs) are plant-specific key signaling molecules that respond to various stimuli and are involved in the synthesis of secondary metabolites. However, little is known about the JA signal pathway, especially in economically significant medicinal plants. To determine the functions of novel genes that participate in the JA-mediated accumulation of secondary metabolites, we examined the metabolomic and transcriptomic signatures from Salvia miltiorrhiza.

[SPME-GC-MS combined with Kovat's retention index analysis for volatile components in Pistacia chinensis].

Objective: To analyze and compare the volatile components in fruits and leaves of Pistacia chinesis.

Methods: The volatile components were extracted from the fruits and leaves of Pistacia chinesis by solid-phrase microextration, and were analyzed and identified by gas chromatography-mass spectrometry(GC-MS) combined with Kovat's retention index. The relative content of each component was calculated by normalization method.

[Antiosteoporotic effects of naringenin on ovariectomy-induced osteoporosis in rat].

To investigate the effect of naringenin on ovariectomy-induced postmenopausal osteoporosis comprehensively and systemically, thirty-two virgin Sprague-Dawley rats about 3-month-old were used and randomly divided into 4 groups: sham control group (Sham), OVX control group (OVX), naringenin treatment group and 17β-estradiol (E2) treatment group. After 12 weeks treatment with different drugs, 24 h urine were collected, organs were weighed and the organ indies were computed. Uterine pathological changes were observed by making paraffin section.

Ergosterol reverses multidrug resistance in SGC7901/Adr cells.

Multidrug resistance (MDR) is a major obstacle in the chemotherapeutic treatment of tumors. Elevated expression of the P-glycoprotein (P-gp) transporter is associated with MDR and responsible for the resistance of tumor cells against a variety of anticancer drugs. In this study, the reversal effect of ergosterol (Erg) on SGC7901/Adr cells was investigated.

Rapid analysis of fatty acid profiles in raw nuts and seeds by microwave-ultrasonic synergistic in situ extraction-derivatisation and gas chromatography-mass spectrometry.

Based on microwave-ultrasonic synergistic in situ extraction-derivatisation (MUED), gas chromatography-mass spectrometry was proposed for rapid analysis of fatty acid profiles in raw nut and seed materials. Several critical experimental parameters for MUED, including reaction temperature, microwave power, amounts of catalyst and derivatisation reagent, have been optimised using response surface methodology. The results showed that the chromatographic peak areas of total fatty acids and the content of total unsaturated fatty acids obtained with MUED were markedly higher than those obtained by the conventional method (P<0.

Analysis of essential oils from different organs of Scutellaria baicalensis.

Objective: To evaluate the differences among essential oils from the fresh flowers, stem leaves, roots and seeds of Scutellaria baicalensis.

Methods: The oils was obtained by hydrodistillation and identified by gas chromatography-mass spectrometry (GC-MS) and the Kovats retention index. The relative contents of the components were determined by the peak-area normalization method adopted in gas chromatography.

[Research on different processings of Scutellaria baicalensis Georgi].

Objective: Comparing the different processings of S. baicalensis Georgi with fresh herb.

Methods: Watering, cooking and steaming method were adopted and the contents of flavonoids was determined by HPLC.