Publications by authors named "Shuang-Luo Xia"
Aim: To reveal new tumor markers and target genes from differentially expressed genes of primary tumor samples using cDNA microarray.
Methods: The (33 )P labeled cDNAs were synthesized by reverse transcription of message RNA from the liver cancerous tissue and adjacent non-cancerous liver tissue from the same patient and used to hybridize to LifeGrid 1.0 cDNA microarray blot containing 8400 known and unique human cDNA gene targets, and an expression profile of genes was produced in one paired human liver tumor tissue.
Detection of Lis1 Gene Frame Shift Mutation in Human Hepatocarcinoma.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
January 2000
Article Synopsis
- The GST fusion protein expression system, along with the protein truncation test (PTT) protocol, was employed to identify gene frame shift mutations in the Lis1 gene from hepatocarcinoma samples.
- Cloning the RT-PCR products into a GST expression vector and expressing them in E. coli led to the unexpected production of a truncated 33 kD protein instead of the expected 71 kD full-length protein.
- Sequencing revealed an insertion mutation that caused premature termination of translation, demonstrating that the enhanced PTT assay is a quick and effective method for detecting frame shift mutations in genes.
Cloning and Characterization of fup1, A Gene Highly Expressed in Hepatocellular Carcinoma.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
January 2001
Primary hepatocellular carcinoma(HCC) is one of the common malignant tumors in China. In our previous work, a gene named fup1(function-unknown protein 1) was isolated that was expressed differently in HCC and in normal liver. We assumed that it might be a candidate oncogene for the HCC.
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