[Research advances on RB1 gene].

The retinoblastoma1 (RB1) gene is the first cloned tumor suppressor gene. As a negative regulator of the cell cycle, RB1 gene could maintain a balance between cell growth and development through binding to transcription factors and regulating the expression of genes involved in cell proliferation and differentiation. Thus, it is involved in cell cycle, cell senescence, growth arrest, apoptosis and differentiation.

[Purification of human MMP-1 fusion protein and clinical detection of MMP-1 by its polyclonal antibody].

Objectives: To obtain the fusion protein of MMP-1 and the rabbit-anti-human matrix metalloproteinase-1(MMP-1) polyclonal antibody, and to detect the serum MMP-1 in patients by this polyclonal antibody.

Methods: The fusion protein was purified by affinity chromatography. Two rabbits were immunized with the purified fusion protein, and the immune sera of rabbits were collected.

[Gene expression profile of peripheral blood monocyte in patients with fulminant hepatitis B by cDNA microarray].

Objective: To investigate peripheral blood monocyte (PBMC) gene expression profile in patients with fulminant hepatic failure (FHF) by cDNA microarray.

Methods: Microarrays consisting of 8,192 human cDNAs and labelled cDNAs prepared from PBMC in both 10 FHF patients and 10 asymtomatic surface antigen carriers (ASC) were applied to analyze gene expression. Relative ratios of gene expression in individuals were obtained by comparing the hybridization results, by GenePix 4000B scanning and by ImaGene3.