[Biological and genome characteristics of a novel broad host-range phage ΦEP1 infecting enteroinvasive ].

We analyzed the biological and genome characteristics of a phage infecting enteroinvasive (EIEC), aiming to provide resources and a reference for the prevention and treatment of EIEC. With the EIEC preserved in our laboratory as the host bacterium, one strain of phage was isolated from the effluent sample from a chicken farm in Huzhou, Zhejiang and named ΦEP1. The titer, optimal multiplicity of infection, one-step growth curve, temperature, pH value, chloroform and bile salt sensitivity of ΦEP1 were determined by the double-layer agar plate method.

Characterization and comparative genomics analysis of RepA_N multi-resistance plasmids carrying from .

Introduction: This research aimed to investigate the antibiotic resistance of from swine farms in Zhejiang Province and the prevalence and transmission mechanism of oxazolidone resistance gene .

Method: A total of 226 were isolated and their resistance to 14 antibiotics was detected by broth microdilution. The resistance genes were detected by PCR.

The diversity in antimicrobial resistance of MDR among Chinese broiler and laying farms and two positive plasmids revealed their resistance-transmission risk.

This research aimed to investigate the microbial composition and diversity of antimicrobial resistance genes (ARGs) found in Chinese broiler and layer family poultry farms. We focused on the differences in resistance phenotypes and genotypes of multidrug-resistant (MDRE) isolated from the two farming environments and the existence and transmissibility of colistin resistance gene . Metagenomic analysis showed that and were the dominant bacteria in broiler and layer farms.

The Clustered Regularly Interspaced Short Palindromic Repeats-Associated System and Its Relationship With Mobile Genetic Elements in .

Microorganisms have developed many strategies in the process of long-term defense against external attacks, one of which is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) bacterial immunological system. In this study, the whole genome of 300 strains of was collected, the CRISPR-Cas system in the strains was statistically analyzed, and the types and structures of CRISPR system in were explored, as well as the correlation between CRISPR and mobile genetic elements (MGEs). Through principal component analysis (PCA), we found that Cas gene, plasmids, integron, IS, IS, and enzymes of DNA metabolism were closely related to CRISPR-Cas.