Pickering emulsion systems are an advanced platform for efficient fine chemical production in biphasic enzymatic catalysis, while their applications are currently limited to a few commercial enzyme classes. Herein, we designed an anchor peptide-based immobilization strategy for Pickering emulsions to achieve efficient glycosylation of natural products by glycosyltransferases (GTs). Firstly, through enzyme mining, natural GTs were utilized to synthesize pharmaceutically important acacetin glucoside and galactoside.
View Article and Find Full Text PDFThe improvement of enzyme thermostability often accompanies the decreased activity due to the loss of the key regions' flexibility. As a representative structure, unlocking the potential of loop dynamics will not only provide new ideas for stabilization strategies, but also help to deepen the understanding of the relationship between enzyme structural dynamics and function. In this study, a creative "hook loop dynamics engineering" (HLoD) strategy was successfully proposed for simultaneously improving the thermostability and maintaining activity of the model enzyme, Candida Antarctica lipase B.
View Article and Find Full Text PDFCytochrome P450s are heme-thiolate enzymes that have been broadly applied in pharmaceutical and biosynthesis because of their efficient oxidation at inert carbons. Extensive engineering campaigns are applied to P450s to explore new non-natural substrates and reactions; however, achieving high coupling efficiency is one of the main challenges. The undesirable uncoupling reactions result in the extra consumption of expensive cofactor NAD(P)H, and lead to the accumulation of reactive oxygen species and the inactivation of enzymes and organisms.
View Article and Find Full Text PDFCytochromes P450 have gained much interest for their broad substrate scope in the catalysis of oxidation reactions for pharmaceuticals, plastics, and hormones. However, achieving high coupling efficiency by the engineering of P450s is still a big challenge. The presence of extra water around the active site is deemed to be related to uncoupling.
View Article and Find Full Text PDFBioresour Bioprocess
April 2021
An active site is normally located inside enzymes, hence substrates should go through a tunnel to access the active site. Tunnel engineering is a powerful strategy for refining the catalytic properties of enzymes. Here, P450HI (Q85H/V170I) derived from hydroxylase P450 from Bacillus subtilis was chosen as the study model, which is reported as a potential decarboxylase.
View Article and Find Full Text PDFEnzyme Microb Technol
December 2019
Musky macrolactones are an important group of compounds used in high-valued perfumery. An enzymatic cascade reaction including cytochrome P450 hydroxylase and lipase was explored to biosynthesize musky macrolactones. Firstly, fatty acids were hydroxylated by P450 hydroxylase to produce the corresponding ω-hydroxy fatty acids.
View Article and Find Full Text PDFFatty acids are versatile precursors for fuels, fine chemicals, polymers, perfumes, etc. The properties and applications of fatty acid derivatives depend on chain length and on functional groups and their positions. To tailor fatty acids for desired properties, an engineered P450 monooxygenase has been employed here for enhanced selective hydroxylation of fatty acids.
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