The larva and adult of use differential gustatory receptors to sense sucrose.

Almost all herbivorous insects feed on plants and use sucrose as a feeding stimulant, but the molecular basis of their sucrose reception remains unclear. as a notorious crop pest worldwide mainly feeds on reproductive organs of many plant species in the larval stage, and its adult draws nectar. In this study, we determined that the sucrose sensory neurons located in the contact chemosensilla on larval maxillary galea were 100-1000 times more sensitive to sucrose than those on adult antennae, tarsi, and proboscis.

Periodontitis and osteoporosis: a two-sample Mendelian randomization analysis.

The incidences of periodontitis and osteoporosis are rising worldwide. Observational studies have shown that periodontitis is associated with increased risk of osteoporosis. We performed a Mendelian randomization (MR) study to genetically investigate the causality of periodontitis on osteoporosis.

and HPV16 coinfection may contribute to the development of cervical cancer.

Persistent human papillomavirus HPV infection is a necessary but insufficient condition for cervical cancer. Microorganisms are crucial environmental factors in cancers susceptibility and progression, recently attracting considerable attention. This study aimed to determine the infection status and relationship between high-risk HPV (HR-HPV) and lower genital tract infectious pathogens in cervical cancer and its precursors.

Phakellisins A-E, cyclopeptides from a marine sponge Phakellia sp. guided by LC-MS.

A chemical investigation of the marine sponge Phakellia sp. from the South China Sea yielded five new cyclopeptides, phakellisins A-E (1-5). Structures of these compounds were determined by comprehensive analysis of 1D/2D NMR, HRESIMS/MS spectroscopic data and the advanced Marfey's method.

LC-MS-Guided Isolation of Cyanogripeptides A-C, Cyclolipopeptides with β-Methyl-Leucine Residues, from an LHW52806.

Cyanogripeptides A-C (-), three new cyclolipopeptides with unusual β-methyl-leucine residues, were identified from an LHW52806 using an LC-MS-guided strategy. The structures of compounds - were elucidated by 1D/2D NMR, HR-MS/MS, and the advanced Marfey's method. The absolute configuration of the β-methyl-leucine residue was determined by a combination of stereoselective biosynthesis of (2,3)-β-methyl-leucine, racemization to its epimer (2,3)-β-methyl-leucine, and the advanced Marfey's method.

[Effects of electroacupuncture on ethology, microglia activation and P2X7 receptor expression in periaqueductal gray in rats with migraine].

Objective: To observe the effects of electroacupuncture(EA) at "Fengchi"(GB20) on the ethology, microglia activation and P2X7 receptor(P2X7R) expression in the periaqueductal gray(PAG) in recurrent migraine rat model, so as to explore the underlying mechanism of EA reducing central sensitization of migraine.

Methods: Thirty-six male SD rats were randomly divided into control, model and EA groups, with 12 rats in each group. Recurrent migraine model was induced using repea-ted dural electrical stimulation once another day(the 1, 3, 5, 7 and 9 days), for a total of 5 times; rats in the EA group received EA treatment(2 Hz/15 Hz, 0.

A Design Method of Two-Dimensional Subwavelength Grating Filter Based on Deep Learning Series Feedback Neural Network.

Subwavelength grating structure has excellent filtering characteristics, and its traditional design method needs a lot of computational costs. This work proposed a design method of two-dimensional subwavelength grating filter based on a series feedback neural network, which can realize forward simulation and backward design. It was programed in Python to study the filtering characteristics of two-dimensional subwavelength grating in the range of 0.

Functional analysis of a bitter gustatory receptor highly expressed in the larval maxillary galea of Helicoverpa armigera.

Many plant secondary substances are feeding deterrents for insects and play a key role in the selection of host plants. The taste sensilla of phytophagous insects contain gustatory sensory neurons sensitive to deterrents but the molecular basis of deterrent chemoreception remains unknown. We investigated the function of Gr180, the most highly expressed bitter gustatory receptor in the maxillary galea of Helicoverpa armigera larvae.

[Identification of key enzyme genes involved in biosynthesis pathways of lignan and lignin in Eucommia ulmoides based on transcriptome assembly].

Lignan is the main medicinal component of Eucommia ulmoides, and lignin is involved in the defense of plants against diseases and insect pests.They are synthesized from coniferyl alcohol with the help of dirigent(DIR) and peroxidase(POD), respectively.In this study, transcriptome assembly of stems and leaves of E.

Novel High-Temperature-Resistant Phosphates: Thermal Ablation Behavior of La-Al System Phosphates at 2000 °C.

The saturation-free and directionless cross-linking and interpenetration processes between La and [(HPO)2Al(HPO)]-plasma in La-Al phosphate by mixing Al(OH), CrO, and HO dissolved in HPO and LaO as a curing accelerator, as well as the thermal stability of the La-Al phosphate bulk materials and the evolutions of the phase composition and morphology at different temperatures were studied using thermogravimetric/differential scanning calorimetry under different temperatures in a muffle furnace. The La-Al phosphates showed good thermal stability, and the thermal weight loss rate of the materials decreased from 18% before heat treatment to ∼2% after heat treatment. In addition, the La-Al phosphate bulk material showed excellent resistance to ablation when subjected to ablation by an oxyacetylene flame at 2000 °C for 30 s.

Axinellasins A-D, Immunosuppressive Cycloheptapeptide Diastereomers, Discovered via a Precursor Ion Scanning-Supercritical Fluid Chromatography Strategy from the Marine Sponge species.

The precursor ion scanning-supercritical fluid chromatography (PI-SFC) method was applied to explore new methionine sulfoxide-containing cycloheptapeptides, axinellasins A-D (-), from the marine sponge sp. Their structures, including absolute configurations, were elucidated by detailed spectroscopic analyses and X-ray crystallography. The total synthesis of was completed via an Fmoc solid/solution-phase synthesis.

Correction: A facile strategy to realize a single/double photon excitation-dependent photosensitizer for imaging-guided phototherapy against HeLa cancer cells at separate irradiation channels.

Correction for 'A facile strategy to realize a single/double photon excitation-dependent photosensitizer for imaging-guided phototherapy against HeLa cancer cells at separate irradiation channels' by Lin Kong et al., Chem. Commun.

The effect of fluid shear stress on fibroblasts and stem cells on plane and groove topographies.

In this study, we aimed to study the effect of fluid shear stress on fibroblasts and BMSCs on plane and groove topographies. The results showed that 0.6-Hz stress had the greatest influence on the alignment, polarity, migration and adhesion of fibroblasts on plane by increasing the expression of reoriented actin and vinculin; whereas 1.

Oxidation of Sodium Deoxycholate Catalyzed by Gold Nanoparticles and Chiral Recognition Performances of Bile Salt Micelles.

Au nanoparticles (NPs) were prepared by UV light irradiation of a mixed solution of HAuCl and sodium deoxycholate (NaDC) under alkaline condition, in which NaDC served as both reducing agent and capping agent. The reaction was monitored by circular dichroism (CD) spectra, and it was found that the formed gold NPs could catalyze the oxidation of NaDC. A CD signal at ~283 nm in the UV region was observed for the oxidation product of NaDC.

A facile strategy to realize a single/double photon excitation-dependent photosensitizer for imaging-guided phototherapy against HeLa cancer cells at separate irradiation channels.

A novel difluoroboron fluorophore with an electron donor-acceptor conjugated structure was synthesized with 26.5% fluorescence quantum yield, 18 035 GM two-photon absorbing cross-section, and undetectable two-photon fluorescence, resulting in 25% O quantum yield. The unique optical behavior of CNFBBN enabled one-photon fluorescence imaging and two-photon phototherapy against HeLa cancer cells, irradiated at separate wavelengths.

Effects of cyclic fluid stress at different frequencies on behaviors of cells incubated on titanium alloy.

The orthopedic external fixation is always in dynamic mechanical environment with the somatic movement. We used a self-designed mini oscillator to simulate this condition by providing the reciprocating cyclic fluid stress, and observed the behavioral responses of fibroblasts implanted on titanium alloy plane to the stress at different frequencies, including 0.2 Hz, 0.

A determining factor for insect feeding preference in the silkworm, Bombyx mori.

Feeding preference is critical for insect adaptation and survival. However, little is known regarding the determination of insect feeding preference, and the genetic basis is poorly understood. As a model lepidopteran insect with economic importance, the domesticated silkworm, Bombyx mori, is a well-known monophagous insect that predominantly feeds on fresh mulberry leaves.

A Surface Mediated Supramolecular Chiral Phenomenon for Recognition of l- and d-Cysteine.

Chiral recognition is of fundamental importance in chemistry and life sciences and the principle of chiral recognition is instructive in chiral separation and enantioselective catalysis. Non-chiral Ag nanoparticles (NPs) conjugated with chiral cysteine (Cys) molecules demonstrate strong circular dichroism (CD) responses in the UV range. The optical activities of the l-/d-Cys capped Ag NPs are associated with the formation of order arrangements of chiral molecules on the surface of Ag NPs, which are promoted by the electrostatic attraction and hydrogen bonding interaction.

Synthesis and Plasmonic Chiroptical Studies of Sodium Deoxycholate Modified Silver Nanoparticles.

Sodium deoxycholate modified silver nanoparticles prepared in the presence of sodium deoxycholate as a chiral inducer exhibit plasmonic circular dichroism (CD) signals. The plasmon-induced chirality arises from the presence of chiral molecules (sodium deoxycholate) on the surface of Ag nanoparticles, which transfer their chiral properties to the visible wavelength range due to the Coulomb interactions between the chiral molecules and plasmonic nanoparticles. The prepared Ag nanoparticles (NPs) exhibit distinct line shapes of plasmonic CD, which can be tailored by varying the pH values of the solutions.

[Role of miR137-MITF in Prognostic Analysis of Multiple Myeloma].

Unlabelled: Objectiive：To explore the effect of miR137 target gene MITF on the prognosis of multiple myeloma (MM).

Methods: The target genes of miR137 were predicted by software, the GFP analysis was carried out for detecting MITF as the prognosis of multiple myeloma. The cell line overexpressing miR137 in MM cell line was constructed.

Quantitative detection of circulating nucleophosmin mutations DNA in the plasma of patients with acute myeloid leukemia.

Objective: The aim of this study was to quantify the copies of circulating nucleophosmin (NPM) mutations DNA in the plasma of patients with acute myeloid leukemia (AML) and to explore the association of circulating NPM mutation levels with clinical characteristics.

Design And Methods: The presence of NPM mutations in 100 Chinese patients newly diagnosed with AML were identified by RT-PCR and sequencing analysis. Copies of circulating NPM mutation A (NPM mut.

Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway.

The RNA-binding protein Musashi2 (Msi2) has been identified as a master regulator within a variety of stem cell populations via the regulation of translational gene expression. A recent study has suggested that Msi2 is strongly expressed in leukemic cells of acute myeloid leukemia patients, and elevated Msi2 is associated with poor prognosis. However, the potential role of Msi2 in leukemogenesis is still not well understood.

[Detecting the cytotoxicities of five bisphenol A analogues to the MCF-7 human breast carcinoma cell line through different endpoints].

As the main synthetic raw materials of polycarbonate, bisphenol A (BPA) and its analogues have been important issues in environmental pollution. The current studies focus mainly on BPA's estrogen effects and little on their cytotoxic effects. To assess the cytotoxicities of the five BPA analogues, we employed the MTS assay to determine the inhibition toxicity to MCF-7 (ER-), 2,4-dinitrophenylhydrazine assay to determine the release rate of lactate dehydrogenase (LDH) escaping into cell culture medium, and single cell gel electrophoresis assay (SCGE) to detect DNA damage.

Evaluation of anti-quorum-sensing activity of fermentation metabolites from different strains of a medicinal mushroom, Phellinus igniarius.

Aims: This study aimed to evaluate the different abilities of various Phellinus igniarius strains inhibiting quorum sensing (QS), to search for novel QS inhibitors from them and to analyze their inhibitory activity, with a view to their possible use in controlling infections.

Methods: The bioactive metabolites produced by P. igniarius cultures were tested for their abilities to inhibit QS-regulated behavior.