6-methyladnosine of vRNA facilitates influenza A virus replication by promoting the interaction of vRNA with polymerase proteins.

6-methyladnosine (mA) modification is present in both positive- and negative-strand RNA of influenza A virus (IAV) and affects the replication and pathogenicity of IAV. However, little is known about the regulatory mechanism of mA in IAV RNA. In the present study, we identified the mA methylation of the viral RNA of different IAV subtypes and confirmed that mA modification promotes the polymerase activity and replication of IAV.

A Novel Disulfidptosis-Related Diagnostic Gene Signature and Differential Expression Validation in Ischaemic Cardiomyopathy.

Ischaemic cardiomyopathy (IC) predominantly arises from prolonged deprivation of oxygen in the coronary arteries, resulting in compromised cardiac contractility or relaxation. This study investigates the role of disulfidptosis-associated genes (DiGs) in IC. Through the analysis of datasets GSE5406 and GSE57338, we explored the association between DiGs and immune characteristics to identify crucial genes contributing to IC development.

Patterns and outcomes of early and late recurrence after hepatectomy for hepatocellular carcinoma with microvascular invasion: a multicenter study in China.

Background: A few studies focus on the long-term outcomes and surveillance strategies for patients with hepatocellular carcinoma (HCC) and microvascular invasion (MVI) who experience postoperative recurrence. The aim of this study was to explore the patterns and prognosis of early and late recurrence (ER and LR) after hepatectomy of such patients.

Methods: Consecutive patients with HCC and MVI after hepatectomy from 26 centers in China from 2009 to 2020 were included.

Rationally Fabricated Ce-Mn@ZrO-SO Catalyst Boosts the Efficient Destruction of Chlorobenzene with SO Impurity: Synergy of Surface SO and Acidic Sites.

The catalytic deactivation caused by SO impurity remains a great challenge in the efficient destruction of industrial chlorinated volatile organic compounds (CVOCs). Herein, a Ce-Mn@ZrO-SO catalyst with a Ce-O-Mn active system and ZrO-SO protective layer was rationally engineered, which exhibits superior activity for chlorobenzene (CB) and SO cotreatment at 228 °C, achieving 90% CB mineralization─over 80% higher than that of the CeO catalyst. In situ characterization and theoretical calculation results reveal that the SO groups not only inhibit the adsorption of SO molecules through steric hindrance and electrostatic repulsion but also act as the Brønsted acid sites (BAS) to promote C-Cl cleavage of chlorobenzene (CB) and accelerate the desorption of Cl radicals as inorganic chlorine (HCl and Cl).

Multimodal magnetic resonance imaging analysis of early mild cognitive impairment.

Background: Early mild cognitive impairment (EMCI) represents a prodromal stage of dementia, and early detection is crucial for delaying dementia progression. However, accurately identifying its neuroimaging features remains challenging.

Objective: To comprehensively evaluate structural and functional neuroimaging changes in EMCI using multimodal magnetic resonance imaging (MRI) techniques.

Identification of Cardiometabolic Protein Biomarkers for Acute Myocardial Infarction Using Olink Proteomics.

Background: Acute myocardial infarction (AMI) is a critical cardiovascular event characterized by sudden coronary blood flow interruption, leading to myocardial ischemia and necrosis. Despite advances in acute therapeutic measures, understanding the metabolic damage related to AMI, particularly through specific protein expressions, remains limited. This study utilized Olink cardiovascular metabolomics technology to explore cardiovascular metabolism-related protein biomarkers associated with AMI, aiming to address the clinical need for early diagnosis and targeted therapy.

The study on effects of acute exposure to high altitude hypoxia on cognitive function in lowlander.

The investigation of cognitive functions in response to high-altitude exposure has garnered increasing scientific interest. However, it remains unclear whether cognitive abilities are selectively impaired or what are the trends of the function. In this study, we examined the effects of acute exposure to 3800 m on cognition among 20 lowlanders (27.

Modeling diabetic cardiomyopathy using human cardiac organoids: Effects of high glucose and lipid conditions.

Diabetic cardiomyopathy (DCM) is a complex metabolic disorder resulting from chronic hyperglycemia and lipid toxicity, which leads to cardiac dysfunction, fibrosis, inflammation, and mitochondrial impairment. Traditional two-dimensional (2D) cell cultures and animal models have limitations in replicating human cardiac physiology and pathophysiology. In this study, we successfully developed a three-dimensional (3D) model of DCM using cardiac organoids generated from human induced pluripotent stem cells (hiPSCs).

Distinct maturity and spatial distribution of tertiary lymphoid structures in head and neck squamous cell carcinoma: implications for tumor immunity and clinical outcomes.

The influence of tertiary lymphoid structures (TLSs) on disease progression and the response to immunotherapy in head and neck squamous cell carcinoma (HNSCC) is well established, yet the heterogeneity among these structures remains largely unexplored. We utilized digital spatial profiling technology to perform in situ transcriptomic sequencing of TLSs across varying levels of maturation and distinct tumor regions within HNSCC. We assessed the prognostic significance of TLS maturation and spatial distribution in 260 patients with HNSCC through hematoxylin and eosin staining and multiplex immunohistochemistry.

Sensitive detection of fluid biomarkers using adamantylidene 1,2-dioxetane based chemiluminescent probes.

Body fluid analysis is a crucial non-invasive diagnostic tool that offers valuable insights into the body's physiological state and aids in early disease detection. Traditional methods, however, can be costly, time-consuming, and lack sensitivity. To address these issues, fluorescence imaging technology, employing various fluorescent probes, has emerged as a promising alternative.

Artificial Intelligence-Enabled Wearable Devices and Nocturnal Scratching in Mild Atopic Dermatitis.

Importance: Although more than 1 in 10 people experience pruritus, there are limited medical technologies that can accurately and continuously quantify and simultaneously reduce scratching behaviors through nonpharmacological methods.

Objective: To evaluate the accuracy and efficacy of an artificial intelligence-enabled wearable sensor with closed-loop haptic feedback to decrease nocturnal scratch in patients with mild atopic dermatitis who report a moderate to severe degree of scratching.

Design, Setting, And Participants: This single-arm 2-stage cohort study with a within-participants design was conducted at a single center and carried out in an at-home environment.

Comparison of laparoscopic and open pancreaticoduodenectomy for distal cholangiocarcinoma and impact factors on textbook outcome.

Background: Distal cholangiocarcinoma (dCCA) is an aggressive malignancy with poor prognosis. This study aimed to compare the short-term and long-term outcomes and "textbook outcome (TO)" between laparoscopic pancreaticoduodenectomy (LPD) and open pancreaticoduodenectomy (OPD) for dCCA after the learning curve of surgeons.

Methods: Clinical and follow-up data were included for dCCA patients treated with LPD or OPD at our center between January 2017 and December 2022.

Decision-making behavior of blood glucose management and its influencing factors in pregnant women with gestational diabetes mellitus.

Background: Currently, most studies only focus on the glucose management level and self- management behavior of pregnant women with gestational diabetes mellitus, but lack analysis and discussion on their decision-making behavior and influencing factors during glucose management.

Aim: This study aimed to investigate the decision-making behavior of blood glucose management and its influencing factors among pregnant women with gestational diabetes mellitus in China.

Methods: This was a prospective study.

Near-Native Imaging of Metal Ion-Initiated Cell State Transition.

Metal ions are indispensable to life, as they can serve as essential enzyme cofactors to drive fundamental biochemical reactions, yet paradoxically, excess is highly toxic. Higher-order cells have evolved functionally distinct organelles that separate and coordinate sophisticated biochemical processes to maintain cellular homeostasis upon metal ion stimuli. Here, we uncover the remodeling of subcellular architecture and organellar interactome in yeast initiated by several metal ion stimulations, relying on near-native three-dimensional imaging, cryo-soft X-ray tomography.

Clinical Characteristics of Ear Chondroid Syringoma: A Report of 2 Cases and a Literature Review.

Chondroid syringoma, a mixed tumor of the skin, is an acquired hamartoma that differentiates into hair follicles, sebaceous glands, and apocrine sweat glands. Chondroid syringoma in the ear region is exceptionally rare. However, we present 2 cases of ear chondroid syringoma: 1 involving multiple lesions in the external auditory canal and the other including a single lesion behind the auricle.

Resolving the Nanostructure of Carbon Nitride-Supported Single-Atom Catalysts.

Single-atom catalysts (SACs) are gathering significant attention in chemistry due to their unique properties, offering uniform active site distribution and enhanced selectivity. However, their precise structure often remains unclear, with multiple models proposed in the literature. Understanding the coordination environment of the active site at the atomic level is crucial for explaining catalytic activity.

The perception of artificial intelligence and infertility care among patients undergoing fertility treatment.

Purpose: To characterize the opinions of patients undergoing infertility treatment on the use of artificial intelligence (AI) in their care.

Methods: Patients planning or undergoing in vitro fertilization (IVF) or frozen embryo transfers were invited to complete an anonymous electronic survey from April to June 2024. The survey collected demographics, technological affinity, general perception of AI, and its applications to fertility care.

Melanoma toolkit for early detection for primary care clinicians: a 1-year follow-up on outcomes.

Introduction: Primary care providers or clinicians (PCPs) have the potential to assist dermatologists in screening patients at risk for skin cancer, but require training to appropriately identify higher-risk patients, perform skin checks, recognize and biopsy concerning lesions, interpret pathology results, document the exam, and bill for the service. Very few validated dermatology training programs exist for PCPs and those that are available focus primarily on one emphasis area, which results in variable efficacy and single-topic limited scope.

Methods: We have created a free, online, continuing education program (Melanoma Toolkit for Early Detection, MTED) that allows learners to choose from a variety of multimedia tools (image recognition, videos, written material, in-person seminars, self-tests, etc.

Mitochondria-Nucleus Migration Probe for Ultrasensitive Monitoring of mtDNA Damage in Living Cells.

Mitochondrial DNA (mtDNA) damage is a prevalent phenomenon that has been proven to be implicated in a wide spectrum of diseases. However, the progressive attenuation of probe signals in response to mtDNA damage within living cells inherently limits the sensitivity and precision of current probes for detecting mtDNA damage. Herein, we employ an innovative organelle signal ratio imaging approach, utilizing the mitochondria-nucleus migration probe MCQ, to achieve unparalleled sensitivity in detecting mtDNA damage in living cells.

Glycated hemoglobin A1c and cognitive impairment in complex chronic patients: A cross-sectional study.

Objective: This study examines the relationship between Glycated hemoglobin A1c (HbA1c) levels and cognitive impairment in elderly patients with complex chronic conditions, a link previously unclear.

Design: This is a cross-sectional study.

Material And Methods: The data from 2,366 patients in Catalonia (2013-2017) from the Dryad database.

Differential Expression Spectrum of circRNA in Plasma Exosomes in Dilated Cardiomyopathy With Heart Failure.

Dilated cardiomyopathy (DCM), a form of non-ischaemic myocardial disease, is characterised by structural and functional cardiac abnormalities. As defined by the World Health Organisation, DCM constitutes a significant cardiac pathology, leading to increased morbidity and mortality due to complications such as heart failure and arrhythmias. The diagnostic process for DCM predominantly employs echocardiography and MRI, with biomarkers like NT-pro BNP and troponin providing supportive, yet non-specific, evidence.

Multisystem clinicopathologic and genetic analysis of MELAS.

Background And Objectives: Mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS) syndrome is a maternally inherited mitochondrial disorder that mostly affects the central nervous system and skeletal muscle. This study provides a comprehensive summary of the clinical symptoms, multisystemic pathogenesis, and genetic characteristics of MELAS syndrome. The aim was to improve comprehension of clinical practice and gain a deeper understanding of the latest pathophysiological theories.

Identification and validation of differentially expressed disulfidptosis-related genes in hypertrophic cardiomyopathy.

Hypertrophic cardiomyopathy (HCM) is one of the most common cardiovascular diseases with no effective treatment due to its complex pathogenesis. A novel cell death, disulfidptosis, has been extensively studied in the cancer field but rarely in cardiovascular diseases. This study revealed the potential relationship between disulfidptosis and hypertrophic cardiomyopathy and put forward a predictive model containing disulfidptosis-associated genes (DRGs) of GYS1, MYH10, PDMIL1, SLC3A2, CAPZB, showing excellent performance by SVM machine learning model.

Preparation and Mechanical Properties of (TiCrZrNb)C-SiC Multiphase High-Entropy Ceramics.

Five carbide powders, TiC, CrC, ZrC, NbC and SiC, were selected as raw materials and mixed by dry or wet milling. Then (TiCrZrNb)C-SiC multiphase ceramics were successfully prepared by spark plasma sintering (SPS) at 1900 °C, using D-HECs-1900 (dry milling method) and W-HECs-1900 (wet milling method), respectively. In this study, the effects of the ball milling method on the microstructure and mechanical properties of the multiphase high-entropy ceramics were systematically investigated.