A Novel SERS Label-Free Sensing Strategy for DON and NIV: A DFT Study on the Interaction between DON/NIV and Ag/Au.

In this study, we propose a novel label-free detection strategy based on surface-enhanced Raman spectroscopy (SERS) for detecting deoxynivalenol (DON) and nivalenol (NIV), analyzing the interactions between these fungal toxins and Ag/Au substrates using density functional theory (DFT). The DFT calculation results indicate that the oxygen atoms in DON and NIV molecules exhibit high electronegativity, suggesting that they can serve as active sites in interaction with the metal surfaces in the SERS effect. By constructing DON/NIV-Ag/Au complex models and evaluating their binding energies, we demonstrate the formation of stable complexes, showing characteristic frequency shifts, broadening, and selective enhancement in theoretical Raman spectra.

The BLV-miRNAs pathway of BLV inhibits the expression of Lactoferrin, Lactoperoxidase, Alpha-lactalbumin and Beta-lactoglobulin proteins.

Bovine leukemia virus (BLV) is a widespread virus that decreases milk production and quality in dairy cows. As crucial components of BLV, BLV-encoded microRNAs (BLV-miRNAs) affect BLV replication and may impact the synthesis of Lactoferrin (LTF), Lactoperoxidase (LPO), Alpha-lactalbumin (alpha-LA), and Beta-lactoglobulin (beta-LG). In this study, we investigated the targeting relationship between BLV-miRNAs and LTF, LPO, alpha-LA, and beta-LG in cow's milk.

The effect of bta-miR-1296 on the proliferation and extracellular matrix synthesis of bovine mammary fibroblasts.

Mammary fibrosis in dairy cows is a chronic condition caused by mastitis, and can lead to serious culling of dairy cows resulting in huge economic losses in the dairy industry. MicroRNAs (miRNAs) exert an important role in regulating mammary gland health in dairy cows. This study investigated whether exosomal miRNAs in mammary epithelial cells can regulate the proliferation of bovine mammary fibroblasts (BMFBs) in mastitis.

The Global Epidemiology of Bovine Leukemia Virus: Current Trends and Future Implications.

Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leucosis (EBL), which is the most significant neoplastic disease in cattle. Although EBL has been successfully eradicated in most European countries, infections continue to rise in Argentina, Brazil, Canada, Japan, and the United States. BLV imposes a substantial economic burden on the cattle industry, particularly in dairy farming, as it leads to a decline in animal production performance and increases the risk of disease.

Density Functional Theory Study on the Interaction between Aflatoxin B1/M1 and Gold Substrate.

Aflatoxin M1 (AFM1) and its precursor, Aflatoxin B1 (AFB1), are highly pathogenic and mutagenic substances, making the detection and sensing of AFB1/M1 a long-standing focus of researchers. Among various detection techniques, surface-enhanced Raman spectroscopy (SERS) is considered an ideal method for AFB1/M1 detection due to its ability not only to enhance characteristic frequencies but also to detect shifts in these frequencies with high repeatability. Therefore, we employed density functional theory in conjunction with surface-enhanced Raman spectroscopy to investigate the interaction between AFB1/M1 and a Au substrate in the context of the SERS effect for the first time.

BLV-miR-B1-5p Promotes Adhesion to Mammary Epithelial Cells by Targeting MUC1.

Bovine leukemia virus (BLV) is widely prevalent worldwide and can persistently infect mammary epithelial cells in dairy cows, leading to reduced cellular antimicrobial capacity. BLV-encoded microRNAs (BLV-miRNAs) can modify host genes and promote BLV replication. We previously showed that BLV-miR-B1-5p significantly promoted () adhesion to bovine mammary epithelial (MAC-T) cells; however, the pathway responsible for this effect remained unclear.

The outer membrane protein of Fusobacterium necrophorum, 43K OMP, stimulates inflammatory cytokine production through nuclear factor kappa B activation.

Objective: Fusobacterium necrophorum causes bovine hepatic abscess, foot rot, mastitis, and endometritis. The 43 kDa outer membrane protein (43 K OMP) of F. necrophorum is a porin protein that plays an important role in infections by this bacterium, but the biological function and the pathogenesis of this protein are largely unknown.

Effect of Mineral Element Imbalance on Neutrophil Respiratory Burst Function and Inflammatory and Antioxidant Responses in Sheep.

This study established a model of mineral element homeostatic imbalance and examined the respiratory burst function of peripheral blood neutrophils and inflammatory and antioxidant indicators before and after the imbalance in sheep. The results showed that after an EDTA injection, the number of activated neutrophils in the peripheral blood was significantly higher than that in the control group ( < 0.01).

Effects of imbalance of mineral elements on peripheral neutrophil metabolism in sheep.

The imbalance of mineral element homeostasis in animals is common, causing animal immune dysfunction. Ten female sheep were randomly selected and injected with 4% (w/v) Na2EDTA through a central venous catheter to establish the mineral element imbalance model, then divided into control group (before injection) and Ethylene Diamine Tetraacetic Acid (EDTA) group (after injection). Isolation of peripheral blood neutrophils for mineral elements content determination was done using Inductively coupled plasma-mass spectrometry (ICP-MS) and nontargeted metabolomics analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Consumption of Dietary Fiber with Different Physicochemical Properties during Late Pregnancy Alters the Gut Microbiota and Relieves Constipation in Sow Model.

Constipation is a common problem in sows and women during late pregnancy. Dietary fiber has potential in the regulation of intestinal microbiota, thereby promoting intestinal motility and reducing constipation. However, the effects of fibers with different physicochemical properties on intestinal microbe and constipation during late pregnancy have not been fully explored.

Collaborative elicitation process for sustainable manufacturing: A novel evolution model of green technology innovation path selection of manufacturing enterprises under environmental regulation.

Due to promote manufacturing enterprises to carry out green technology innovation practice smoothly, achieve the goal of energy conservation and emission reduction, and win green competitive advantage, this paper first divides the green technology innovation path into two types, namely internal independent R&D of green technology (IIGT) and external green technology introduction (EGTI), and analyzes the operation mechanism of these two types of paths. Secondly, a two-agent game model of different types of environmental regulation tools on the choice of green technology innovation path of manufacturing enterprises is constructed. To be sure, the manufacturing enterprises include the leader enterprise A and the follower enterprise B.

The chemical adsorption effect of surface enhanced Raman spectroscopy of nitrobenzene and aniline using the density functional theory.

Nitrobenzene and Aniline are representatives of the nitro or amino compounds of benzene, mainly used in the manufacture of dyes, spices, medicines, and so on. Extensive use of Nitrobenzene and Aniline may cause pesticide residue pollution and have carcinogenic effects on organisms. In this paper, the Nitrobenzene and Aniline single molecules and their complexes with gold nanoparticles are studied theoretically by Raman spectroscopy, the surface-enhanced Raman spectroscopy (SERS) and the density functional theory (DFT) simulations.

Estrogen-related Receptor α (ERRα) Functions in The Hypoxic Injury of Microglial Cells.

Introduction: Hypoxia is a common pathological condition after spinal cord injury. Oestrogen-related receptor alpha (ERRα), as a key regulator of energy metabolism and mitochondrial functions, plays an important role in maintaining cell homeostasis. However, its role in hypoxic spinal microglia has not been fully elaborated.

Effects of Hormone, NEFA and SCFA on the Migration of Neutrophils and the Formation of Neutrophil Extracellular Traps in Dairy Cows.

Polymorphonuclear neutrophils (PMN) are the first line of defense against the invasion of foreign pathogenic microorganisms and play an essential role in the immune system of dairy cows. The changes in hormone secretion and metabolites of dairy cows during the perinatal period are the key factors that cause immunosuppression and increased risk of diseases. However, the effects of the hormone, nonesterified fatty acid (NEFA), and short-chain fatty acid (SCFA) on the transmammary epithelial migration of dairy cows and the formation of neutrophil extracellular traps (NETs) have rarely been studied.

Interaction of 43K OMP of Fusobacterium necrophorum with fibronectin mediates adhesion to bovine epithelial cells.

Fusobacterium necrophorum, a Gram-negative anaerobe, is an important bovine pathogen that causes hepatic abscesses, foot rot, mastitis and endometritis. We have previously shown that the 43 kDa outer membrane protein (43 K OMP) of F. necrophorum is a porin protein that plays an important role in bacterial infections; however, the molecular mechanisms by which this protein mediates adhesion remain unclear.

The characteristics of Raman spectroscopy of isomer CBD- and THC-Au nanoparticles using the density functional theory.

The isomers cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) can both be extracted from cannabis. We use density functional theory to study the Raman activity spectra, frontier molecular orbitals, and molecular electrostatic potentials of CBD, THC, and their respective gold complexes. A "selectivity enhancement" phenomenon for the spectral peaks at frequencies of 1144 cm and 1553 cm in the Raman spectrum of the CBD-Au complex, and at frequencies of 865 cm, 1335 cm, and 1553 cm in the Raman spectrum of the THC-Au complex, was observed and explained.

Effect of Copper, Zinc, and Selenium on the Migration of Bovine Neutrophils.

Neutrophils represent the first line of mammary gland defense against invading pathogens by transmigration across the mammary epithelial cell barrier. The effect of trace elements on the migration of bovine neutrophils is not clear. In this study, we investigated the effect of copper (Cu; 0.

Chemical Enhancement Effect of Icotinib-Au Complex Studied by Combined Density Functional Theory and Surface-Enhanced Raman Scattering.

Icotinib is an epidermal growth factor receptor tyrosine kinase inhibitor used in the treatment of non-small cell lung cancer. The charge transfer effect between gold nanoparticles (AuNPs) and icotinib molecules can be used as a model to study the adsorption mechanism between molecules and metal. The adsorption of icotinib on the AuNP surface was confirmed by UV-vis and transmission electron microscopy (TEM) experiments.

OGT upregulates myogenic IL-6 by mediating O-GlcNAcylation of p65 in mouse skeletal muscle under cold exposure.

Cold exposure is an unavoidable and severe challenge for people and animals residing in cold regions of the world, and may lead to hypothermia, drastic changes in systemic metabolism, and inhibition of protein synthesis. O-linked-N-acetylglucoseaminylation (O-GlcNAcylation) directly regulates the activity and function of target proteins involved in multiple biological processes by acting as a stress receptor and nutrient sensor. Therefore, our study aimed to examine whether O-GlcNAcylation affected myogenic IL-6 expression, regulation of energy metabolism, and promotion of survival in mouse skeletal muscle under acute cold exposure conditions.

Effect of BLV Infection on the Immune Function of Polymorphonuclear Neutrophil in Dairy Cows.

Enzootic bovine leukemia is a late-onset, neoplastic infection caused by the bovine leukemia virus (BLV). BLV infection hinders the function of the immune system and induces other diseases, which negatively affects the performance and health of the infected cows. As the first line of defense against invading foreign pathogenic microorganisms, polymorphonuclear neutrophil (PMN) plays a vital role in the immune system of dairy cows.

Ingestion of xylooligosaccharides during the suckling period improve the feed efficiency and hindgut fermentation capacity of piglets after weaning.

Fiber ingestion during the suckling period is helpful for gut development and probiotic colonization. Xylooligosaccharides (Xos) and xylan (Xyl) were selected to investigate the effects of different polymerization degree fiber ingestion on the growth performance and microbiota fermentation capacity of pre- and post-weanling piglets. An fermentation trial was also conducted to verify the microbial fermentation capacity of weanling piglet fecal microbiota.

Transcriptomic Analysis of Circulating Neutrophils in Sheep with Mineral Element Imbalance.

This experiment was designed to investigate the effects of mineral element imbalance on gene transcription in peripheral circulating neutrophils of sheep. Ten female sheep weighing 30 kg of similar age and physiological condition were randomly selected and injected via central venous catheterization with EDTA at a concentration of 4% (W/V) to construct a model of mineral element imbalance. As self-control, the sheep were divided into control [Con, before EDTA injection, time point 0 (T0)], EDTA [12 h after EDTA injection, time point 12 h (T12h)] and recovery [HF, 7 days after injection, time point 7 days (T7d)] groups.

Cold-Induced RNA-Binding Protein Promotes Glucose Metabolism and Reduces Apoptosis by Increasing AKT Phosphorylation in Mouse Skeletal Muscle Under Acute Cold Exposure.

The main danger of cold stress to animals in cold regions is systemic metabolic changes and protein synthesis inhibition. Cold-induced RNA-binding protein is a cold shock protein that is rapidly up-regulated under cold stimulation in contrast to the inhibition of most proteins and participates in multiple cellular physiological activities by regulating targets. Therefore, this study was carried out to investigate the possible mechanism of CIRP-mediated glucose metabolism regulation and survival promotion in skeletal muscle after acute cold exposure.

Exosomal miR-193b-5p as a regulator of LPS-induced inflammation in dairy cow mammary epithelial cells.

Exosomes are a type of extracellular vesicle that act as shuttles, transporting certain genetic information to other cells. MiRNA cargo within exosomes can regulate gene expression at the transcriptional level. The objective of this study was to investigate the exosomal miRNAs that regulate lipopolysaccharide (LPS)-induced inflammation in dairy cow mammary alveolar (Mac-T) cells.