Identification and Functional Analysis of Adipokinetic Hormone Receptor in Ostrinia furnacalis Guenée Larvae Parasitized by Macrocentrus cingulum.

As a typical G protein-coupled receptor, the adipokinetic hormone receptor (AKHR) has seven transmembrane domains (TMDs), and its structure and function are similar to the gonadotropin-releasing hormone receptor (GnRHR) in vertebrates. However, there is a dearth of information on other components of the AKHR signaling pathway and how it functions in the interaction between insect hosts and parasitoids. In this study, we cloned and analyzed the multifunctional Ostrinia furnacalis AKHR (OfAKHR) cDNA (GenBank accession number MF797868).

Pleiotropic immunoregulation by growth-blocking peptide in Ostrinia furnacalis.

Insects rely on their innate immune system to eliminate pathogenic microbes. As a system component, cytokines transmit intercellular signals to control immune responses. Growth-blocking peptide (GBP) is a member of the stress-responsive peptide family of cytokines found in several orders of insects, including Drosophila.

Identification and characteristic analysis of an extracellular signal-regulated kinase from Ostrinia furnacalis Guenée.

The extracellular signal-regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. In the current study, a full-length cDNA (1592bp) encoding the ERK gene (OfERK) was cloned from Ostrinia furnacalis Guenée (GenBank accession number: MF797866). The open reading frame of the OfERK gene encoded 364 amino acids and shared 96.

MiR-2b-3p Downregulated Expression in the Larval Midgut to Decrease Cry1Ac Susceptibility of the Diamondback Moth, (L.).

Crystal (Cry) toxins, produced by , are widely used as effective biological pesticides in agricultural production. However, insects always quickly evolve adaptations against Cry toxins within a few generations. In this study, we focused on the Cry1Ac protoxin activated by protease.

Compressed Imaging Reconstruction Based on Block Compressed Sensing with Conjugate Gradient Smoothed Norm.

Compressed imaging reconstruction technology can reconstruct high-resolution images with a small number of observations by applying the theory of block compressed sensing to traditional optical imaging systems, and the reconstruction algorithm mainly determines its reconstruction accuracy. In this work, we design a reconstruction algorithm based on block compressed sensing with a conjugate gradient smoothed l0 norm termed BCS-CGSL0. The algorithm is divided into two parts.

Characterization and functional analysis of a myeloid differentiation factor 88 in Ostrinia furnacalis Guenée larvae infected by Bacillus thuringiensis.

Myeloid differentiation factor 88 (MyD88) is a pivotal adapter protein involved in activating nuclear factor NF-κB of the Toll pathway in insect innate immunity. MyD88 has been extensively studied in vertebrates and Drosophila. However, the information ascribed to MyD88 in Lepidoptera is scarce.

Application of open-door laminoplasty with ARCH plate fixation in cervical intraspinal tumors.

Background: The open-door laminoplasty is an effective procedure for the treatment of cervical spondylotic myelopathy. However, little information is available about the surgical results of open-door laminoplasty in the treatment of intraspinal tumors. In the present study, we aimed to investigate the clinical effect of open-door laminoplasty with ARCH plate fixation in the treatment of cervical intraspinal tumors.

Culture-negative versus culture-positive in pyogenic spondylitis and analysis of risk factors for relapse.

Objectives: This study aims to compare and analyze the clinical features, diagnosis, treatment and prognosis of culture-negative and culture-positive primary pyogenic spondylitis.

Methods: In a retrospective analysis, 202 cases of adult primary pyogenic spondylitis with complete clinical data in our hospital from January 2013 to January 2020 were divided into two groups according to bacterial culture results: culture negative ( = 126) and culture positive ( = 76). We compare the clinical characteristics, diagnosis, treatment and prognosis of patients with different culture results.

Gut microbiota mediate Plutella xylostella susceptibility to Bt Cry1Ac protoxin is associated with host immune response.

Insect gut microbiotas have a variety of physiological functions for host growth, development, and immunity. Bacillus thuringiensis (Bt) is known to kill insect pests by releasing insecticidal protoxins, which are activated in the insect midgut. However, the interplay among Bt infection, host immunity, and gut microbiota are still unclear.

Studies on 11 Cases of Spinal Epidural Abscess and Literature Review.

Objective: In the present study, we aimed to describe the clinical features, diagnosis, treatment, and prognosis of spinal epidural abscess (SEA).

Methods: The complete clinical data of 11 SEA patients who were treated in our hospital system from January 2015 to June 2018 were retrospectively analyzed. Moreover, the clinical features, diagnosis, treatment, and prognosis of 642 SEA cases collected from the foreign literature from 2010 to 2019 were also investigated.

Transdural circumferential decompression for thoracic spinal stenosis caused by beak-type ossification of the posterior longitudinal ligament: a technical note.

Purpose: Thoracic myelopathy caused by ossification of the posterior longitudinal ligament (OPLL) in the thoracic spine is usually progressive and responds poorly to conservative therapy, making surgery the only effective treatment option. A variety of surgical procedures have been developed to treat thoracic OPLL. However, the optimal surgical approach for removal of thoracic OPLL remains unclear.

Aspergillus spondylitis: case series and literature review.

Background: Spinal fungal infections, especially spinal Aspergillus infections, are rare in the clinic. Here, we introduce the clinical features, diagnosis, treatment, and prognoses of 6 cases of Aspergillus spondylitis.

Methods: We retrospectively analysed the complete clinical data of patients with Aspergillus spondylitis treated in our hospital from January 2013 to January 2020.

The Tripartite Interaction of Host Immunity- Infection-Gut Microbiota.

(Bt) is an important cosmopolitan bacterial entomopathogen, which produces various protein toxins that have been expressed in transgenic crops. The evolved molecular interaction between the insect immune system and gut microbiota is changed during the Bt infection process. The host immune response, such as the expression of induced antimicrobial peptides (AMPs), the melanization response, and the production of reactive oxygen species (ROS), varies with different doses of Bt infection.

Application of Laminoplasty Combined with ARCH Plate in the Treatment of Lumbar Intraspinal Tumors.

Objective: To investigate the bone fusion and clinical effect of laminoplasty combined with ARCH plate fixation in the treatment of lumbar intraspinal tumors.

Methods: This was a clinical study. From June 2017 to January 2019, 24 patients (seven males and 17 females, average age 40 ± 16 years) with lumbar intraspinal tumors underwent laminoplasty combined with ARCH plate fixation in our hospital.

Pathogenicity and Transgenerational Effects of Metarhizium anisopliae on the Demographic Parameters of Aedes albopictus (Culicidae: Diptera).

Dengue fever is a vector-borne infectious disease that spreads swiftly and threatens human lives in several tropical countries. Most of the strategies employed for the control of Aedes albopictus (Skuse) involve synthetic chemicals. The indiscriminate use of synthetic chemicals has led to the development of resistance and is unsafe for human and environmental health.

An immune-responsive PGRP-S1 regulates the expression of antibacterial peptide genes in diamondback moth, Plutella xylostella (L.).

Peptidoglycan recognition proteins (PGRPs) are family of pattern recognition receptors (PRRs) and triggers the innate immune system (IIS) against the microbial infection. Although PGRPs have been intensively studied in model insects, they remain uncharacterized in most of the non-model insects. Here, we cloned and characterized a full-length cDNA of PGRP, from P.

Thoracic psammomatous meningioma with osseous metaplasia: a controversial diagnosis of a case report and literature review.

Background: Spinal psammomatous meningioma with calcification is commonly observed, but distinctive osseous differentiation rarely occurs.

Case Presentation: Here, we described a 52-year-old female complaining of chronic back pain for 5 years. CT and MRI examinations revealed an intradural extramedullary mass at the T4 level.

Dura sac compression due to spinal epidural gas pseudocyst after lumbar decompression surgery: a case report.

Background: Intraspinal gas pseudocyst is rare, especially following spinal surgery. Here we present a case of spinal epidural gas pseudocyst following lumbar decompression surgery, which caused dura sac compression.

Case Presentation: A 52-years-old woman with chronic lumbar pain and radiating numbness of left leg was admitted to our hospital and underwent a posterior lumbar decompression surgery.

MicroRNA expression profiling of Plutella xylostella after challenge with B. thuringiensis.

The diamondback moth, Plutella xylostella, the main pest of brassica crops, has developed resistance to almost all major classes of insecticides as the farmers rely on insecticides to control this pest. An extensive use of broad-spectrum insecticides against P. xylostella promotes the selection of insecticide resistance, destroy natural enemies, and pollute the environment.

Suppresses the Humoral Immune System to Overcome Defense Mechanism of .

has become a notorious pest of cruciferous crops all over the world. Delta-endotoxins of are widely used insecticidal proteins for controlling . However, the interaction mechanism of with the immune system of , at the genomic level, is still unclear.

Genome-Wide Identification of Destruxin A-Responsive Immunity-Related MicroRNAs in Diamondback Moth, .

, a global key pest, is one of the major lepidopteran pests of cruciferous vegetables owing to its strong ability of resistance development to a wide range of insecticides. Destruxin A, a mycotoxin of the entomopathogenic fungus, , has broad-spectrum insecticidal effects and has been used as an alternative control strategy to reduce harmful effects of insecticides. However, microRNA (miRNA)-regulated reactions against destruxin A have not been elucidated yet.

Genome-Wide Profiling of Immunity-Related miRNAs after Infection.

The development of resistance by to almost all insecticides is of significant concern all over the world. Entomopathogenic fungi such as have been used as an alternative to insecticides. However, the knowledge of miRNA-regulated reactions against entomopathogenic fungi is still in its infant stage.

Identification of immunity-related genes in Plutella xylostella in response to fungal peptide destruxin A: RNA-Seq and DGE analysis.

Plutella xylostella has become the major lepidopteran pest of Brassica owing to its strong ability of resistance development to a wide range of insecticides. Destruxin A, a mycotoxin of entomopathogenic fungus, Metarhizium anisopliae, has broad-spectrum insecticidal effects. However, the interaction mechanism of destruxin A with the immune system of P.

Serum miR-34a is a potential diagnostic and prognostic marker for osteosarcoma.

Background And Aims: MicroRNA-34a (miR-34a) has been shown to be a potential diagnostic and prognostic marker for several cancers. In addition, miR-34a has been reported to suppress osteosarcoma cell proliferation in vitro. However, the clinical value of miR-34a is still unknown.

The Entomopathogenic Fungi Plays a Vital Role in Suppressing the Immune System of : RNA-Seq and DGE Analysis of Immunity-Related Genes.

Most, if not all, entomopathogenic fungi have been used as alternative control agents to decrease the insect resistance and harmful effects of the insecticides on the environment. Among them, has also shown great potential to control different insect pests. In the present study, we explored the immune response of to the infection of at different time points by using RNA-Sequencing and differential gene expression technology at the genomic level.