Myeloid dendritic cells and periodontal disease association: integrated study of single-cell sequencing and Mendelian randomization analysis.

Background: Periodontal disease is a widespread inflammatory condition that compromises the supporting structures of the teeth, potentially resulting in tooth loss if left untreated. Despite advancements in therapeutic interventions and an enhanced understanding of its pathophysiology, emerging techniques such as single-cell RNA sequencing (scRNA-seq) and Mendelian randomization (MR) present new opportunities for precision medicine in the management of periodontal disease.

Methods: Data derived from the GSE152042 dataset underwent rigorous quality control, normalization, and dimensionality reduction using Seurat and the MonacoImmuneData framework.

Efficient hairy root induction system of Astragalus membranaceus and significant enhancement of astragalosides via overexpressing AmUGT15.

Astragalus membranaceus hairy roots induced by direct injection of Rhizobium rhizogenes with AmUGT15 overexpressing genes into the stem explants demonstrate enhanced astragaloside biosynthesis Astragalus membranaceus is a widely used medicinal plant, which has important economic, ecological, medicinal, and ornamental values for accumulating various triterpene saponins named astragalosides in roots. Although the hairy root culture technique has been established in A. membranaceus, the molecular regulation of metabolic pathways for improving astragaloside contents was not reported.

Negative pressure assisted rubbing suction technique for the treatment of axillary osmidrosis based on minimally invasive principle.

Background: Axillary Osmidrosis is highly prevalent and occurr in adolescents, bilateral onset, its pathogenesis is related to abnormal secretion function of apocrine glands, with a significant genetic predisposition, the patient's bilateral armpit area can smell a spicy pungent odor, although it does not affect the health, but it is inconvenient to life, socialize. Patients are often accompanied by low self-esteem and urgently require treatment, and most of them have the requirement of minimally invasive treatment and minimal scarring.

Method: In this study, we aimed to evaluate the efficacy of minimally invasive treatment of axillary osmidrosis with refined negative pressure-assisted rubbing suction technique.

Clinical study of matrix vascular component gel combined with vacuum sealing drainage technique in chronic wounds.

Objective: This study investigates the efficacy of the combination of extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel) and vacuum sealing drainage (VSD) on chronic wounds.

Methods: From February 2021 to February 2022, 20 patients with chronic wounds were recruited and were divided into experimental and control groups, with 10 patients in each group. Following debridement, we applied various treatments to all cases for 2 weeks.

Protocol to process crosslinking and immunoprecipitation data into annotated binding sites.

Here, we present a protocol for using Skipper, a pipeline designed to process crosslinking and immunoprecipitation (CLIP) data into annotated binding sites. We describe steps for partitioning annotated transcript regions and fitting data to a beta-binomial model to call windows of enriched binding. From raw CLIP data, we detail how users can map reproducible RNA-binding sites to call enriched windows and perform downstream analysis.

Management of deep sacral and ischial pressure injuries with free-style local perforator flaps: A D+P+DPD model.

Background: Previous reports on the treatment of sacral and ischial pressure injuries have not provided clear algorithms for surgical therapies. The objective of this study was to establish a reconstruction algorithm to guide the selection of an ideal free-style perforator flap that can be tailored to the defect in question.

Methods: We used 23 perforator flaps to reconstruct 14 sacral and 8 ischial defects in 22 patients over 5 years.

Identification of novel loci associated with starch content in maize kernels by a genome-wide association study using an enlarged SNP panel.

Unlabelled: Starch is a major component of cereals, comprising over 70% of dry weight. It serves as a primary carbon source for humans and animals. In addition, starch is an indispensable industrial raw material.

act redundantly with to control spike development and their elite haplotypes may improve wheat grain yield.

Wheat is a staple crop for the world's population, and there is constant pressure to improve grain yield, which is largely determined by plant architecture. SQUAMOSA promotor-binding protein-like (SPL) genes have been widely studied in rice, including their effects on plant architecture, grain development, and grain yield. However, the function of homologous genes in wheat has not been well investigated.

The genetic architecture of prolificacy in maize revealed by association mapping and bulk segregant analysis.

Here, we revealed maize prolificacy highly correlated with domestication and identified a causal gene ZmEN1 located in one novel QTL qGEN261 that regulating maize prolificacy by using multiple-mapping methods. The development of maize prolificacy (EN) is crucial for enhancing yield and breeding specialty varieties. To achieve this goal, we employed a genome-wide association study (GWAS) to analyze the genetic architecture of EN in maize.

Genetic dissection of maize (Zea mays L.) chlorophyll content using multi-locus genome-wide association studies.

Background: The chlorophyll content (CC) is a key factor affecting maize photosynthetic efficiency and the final yield. However, its genetic basis remains unclear. The development of statistical methods has enabled researchers to design and apply various GWAS models, including MLM, MLMM, SUPER, FarmCPU, BLINK and 3VmrMLM.

In vitro exposure to environmentally relevant concentrations of norgestrel affects sperm physiology and reproductive success of the Pacific oyster Crassostrea gigas.

Progestins in aquatic environments are of increasing concern, as shown by the results of toxicological studies on adult invertebrates with external fertilization. However, their potential effects on the gametes and reproductive success of such animals remain largely unknown. Thus, the current study assessed the effect of in vitro exposure of environmentally relevant concentrations (10 ng/L and 1000 ng/L) of norgestrel (NGT) on the sperm of Pacific oyster Crassostrea gigas, analyzing sperm motility, ultrastructure, mitochondrial function, ATP status, characteristic enzyme activities, and DNA integrity underlying fertilization and hatching success.

QTL Mapping for Wheat Seed Dormancy in a Yangmai16/Zhongmai895 Double Haploid Population.

Pre-harvest sprouting (PHS) of wheat reduces grain yield and quality, and it is strongly affected by seed dormancy. Therefore, identification of quantitative trait loci (QTL) for seed dormancy is essential for PHS resistance breeding. A doubled haploid (DH) population, consisting of 174 lines from the cross between Yangmai16 (YM16) and Zhongmai895 (ZM895) was used to detect QTLs for seed dormancy and grain color.

The risk of immediate pneumothorax after CT-guided lung needle biopsy: pleural tail sign as a novel factor.

Background: Pneumothorax is the most frequent complication in computed tomography-guided lung needle biopsy (CT-LNB) and generally appears immediately or within an hour after CT-LNB. Preventing pneumothorax after CT-LNB requires a preoperative evaluation of risk factors. This study investigated risk factors for the occurrence of immediate pneumothorax after CT-LNB.

Norgestrel causes digestive gland injury in the clam Mactra veneriformis: An integrated histological, transcriptomics, and metabolomics study.

The potential adverse effects of progestins on aquatic organisms, especially non-target species, are of increasing concern worldwide. However, the effect and mechanism of progestin toxicity on aquatic invertebrates remain largely unexplored. In the present study, clams Mactra veneriformis were exposed to norgestrel (NGT, 0, 10, and 1000 ng/L), the dominant progestin detected in the aquatic environment, for 21 days.

A modified perforator-based stepladder V-Y advancement flap in the Achilles tendon area for coverage of larger posterior heel defects.

Background: Posterior heel defect coverage is challenging because of the paucity of suitable flaps. The traditional local stepladder V-Y advancement flap is recommended only for small defects because of the lack of an axial pedicle. This study reports our experience of using the perforator-based stepladder V-Y advancement flaps in a larger posterior heel defect repair.

Entropy-stabilized single-atom Pd catalysts via high-entropy fluorite oxide supports.

Single-atom catalysts (SACs) have attracted considerable attention in the catalysis community. However, fabricating intrinsically stable SACs on traditional supports (N-doped carbon, metal oxides, etc.) remains a formidable challenge, especially under high-temperature conditions.

Effects of equibiaxial mechanical stretch on extracellular matrix-related gene expression in human calvarial osteoblasts.

Mechanical stretch commonly promotes craniofacial suture remodeling during interceptive orthodontics. The mechanical responses of osteoblasts in craniofacial sutures play a role in suture remodeling. Moreover, the extracellular matrix (ECM) produced by osteoblasts is crucial for the transduction of mechanical signals that promote cell differentiation.

Barium-promoted hydrothermal stability of monolithic Cu/BEA catalyst for NH-SCR.

As a promising candidate for NOx elimination from the emission of diesel engines, the enhancement effect of Ba on the hydrothermal stability of cordierite supported CuBa/BEA at 600 °C for 48 h was investigated by XRD, NH3-TPD, H2-TPR, XPS, EPR, TEM and in situ DRIFTS. Different properties and amounts of active species are significant factors contributing to the enhanced hydrothermal stability of CuBa/BEA-HT. CuBa/BEA-HT has more Cu2+/Cu+ redox-couples and stronger interactions than Cu/BEA-HT, indicating an excellent redox property of the active species in CuBa/BEA-HT.

Zinc finger transcription factor Sp7/Osterix acts on bone formation and regulates col10a1a expression in zebrafish.

Sp7/Osterix as a zinc finger transcription factor is expressed specifically in osteoblasts. Embryonic lethality of Sp7 knockout mice, however, has prevented from examining the functions of Sp7 in osteoblast and bone formation in live animals. Here we used TALEN, a versatile genome-editing tool, to generate one zebrafish sp7 mutant line.

Symptoms and signs of temporomandibular disorders in patients with knee osteoarthritis.

Objectives: Both temporomandibular disorder (TMD) and knee osteoarthritis (KOA) are prevalent joint diseases; however, an association between them has not been reported. Therefore, this study investigated the prevalence of the specific symptoms and signs of TMDs (SSTs) in patients with KOA.

Methods: In total, 200 patients with KOA and 150 healthy individuals were recruited.

[Research progress on the cellular and molecular mechanisms of tooth eruption].

Tooth eruption is a series of complicated physiological processes occurring once the crown is formed completely, as well as when the tooth moves toward the occasion plane. As such, the tooth moves through the alveolar bone and the oral mucosa until it finally reaches its functional position. Most studies indicate that the process of tooth eruption involves the alveolar bone, dental follicles, osteoclasts, osteoblasts, and multiple cytokines.

Orthodontists' and laypeople's perception of smile height aesthetics in relation to varying degrees of transverse cant of anterior teeth.

Aims: To determine the effect of varying the transverse cant of the anterior teeth on orthodontists' and laypeople's perceptions of smile aesthetics, and the influence that smile height has on this perception.

Materials And Methods: A 20-year-old Chinese female with an aesthetic smile and normal occlusion was chosen and agreed to participate. Digital pictures of her posed smile were taken and manipulated to create three smile height variations: low, medium, or high.

[Skeletal Class Ⅲ patients treated with Fränkel function regulator type Ⅲ in the early and late mixed dentition].

Objective: To investigate the outcome of skeletal Class Ⅲ patients treated with Fränkel function regulator type Ⅲ (FR Ⅲ)in the early mixed and late mixed dentition.

Methods: The samples consisted of 45 mild and moderate skeletal Class Ⅲ patients(26 males, 19 females; meanage, [7.9±1.