Design of hepadnavirus core protein-based chimeric virus-like particles carrying epitopes from respiratory syncytial virus.

Respiratory syncytial virus (RSV) is one of the most important pathogens causing respiratory tract infection in humans, especially in infants and the elderly. The identification and structural resolution of the potent neutralizing epitopes on RSV fusion (F) protein enable an "epitope-focused" vaccine design. However, the display of RSV F epitope II on the surface of the widely-used human hepatitis B virus core antigen (HBcAg) has failed to induce neutralizing antibody response in mice.

Impact of bariatric surgery on glucose and lipid metabolism and liver and kidney function in food-induced obese diabetic rats.

Background: Obesity usually causes diabetes mellitus (DM) and is a serious danger to human health. Type 2 DM (T2DM) mostly occurs along with obesity. Foodborne obesity-induced DM is caused by an excessive long-term diet and surplus energy.

Concomitant gallstone disease was not associated with long-term outcomes in ursodeoxycholic acid-treated patients with primary biliary cholangitis.

Objectives: Primary biliary cholangitis (PBC) is a rare disease characterized by intrahepatic cholestasis, whereas gallstone disease (GD) is common. In this study, we aimed to investigate the prevalence and impact of GD on the prognosis of PBC in China.

Methods: Medical records of the PBC patients were retrospectively reviewed and their follow-up data were obtained via regular structured, standardized telephone interviews.

[Realization and Clinical Study of Robotic Technology in Cardiovascular Interventional Surgery].

The high incidence of cardiovascular diseases is a serious threat to human health, and endovascular surgery has become the standard treatment for most interventional cardiovascular diseases. The robotassisted endovascular surgery system further enhances surgeons' ability to perform minimally invasive endovascular procedures in interventional cardiology. This study presents a new robotic technique for coronary intervention from the perspective of clinical application.

Concomitant extrahepatic autoimmune diseases do not compromise the long-term outcomes of primary biliary cholangitis.

Background: Primary biliary cholangitis (PBC) patients often have concomitant extrahepatic autoimmune (EHA) diseases including Sjögren's syndrome (SS), systemic sclerosis (SSc), rheumatoid arthritis (RA), and autoimmune thyroid disease. The present study aimed to describe the prevalence of EHA diseases in PBC and explore the impact of EHA diseases on the long-term outcomes of PBC in Chinese patients.

Methods: Medical records of PBC patients diagnosed in our institute were retrospectively reviewed.

Optical Density Value and Ratio as Novel Indexes for Nanoemulsion Sterile Filtration Process Control or Characterization.

Sterile filtration is an effective method to remove any microorganisms present during nanoemulsion preparation. However, it lacks effective control parameters. Here, we established a simple and rapid approach for the process control of nanoemulsion sterile filtration by utilizing optical density detection as a process control parameter.

Complete plastome sequence of (L.) P.Royen (Sapotaceae).

iThe plastome of is found to be 158,386 bp long with the typical quadripartite structure of angiosperms, contains two inverted repeats (IRs) of 26,099 bp each, a large single-copy (LSC) region of 87,745 bp, and a small single-copy (SSC) region of 18,443 bp. The plastome contains 114 genes, consisting of 80 unique protein-coding genes, 30 unique tRNA genes, and 4 unique rRNA genes. The overall A/T content in the plastome of M.

[Risk factors of oral Helicobacter pylori infection among children in two kindergartens in Suzhou and the effects of oral cleaning on reducing oral Helicobacter pylori infection].

Objective: This work aimed to identify the risk factors of Helicobacter pylori (H. pylori) infection in preschool children and provide effective measures for the prevention and reduction of the incidence of H. pylori infections.

The biological functions of LGR5 in promoting non-small cell lung cancer progression.

Background: The Leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), which is used as a marker of adult stem cells and colorectal cancer stem cells (CSCs), is closely associated with the progression of non-small cell lung cancer (NSCLC). This study aimed to identify the clinical significance and biological function of LGR5 in NSCLC.

Methods: Quantitative reverse transcription polymerase chain reaction (RT-PCR) was applied to detect the expression of LGR5 and stemness-related genes in 22 NSCLC patients, and the clinical significance of LGR5 in NSCLC progression was estimated by statistical analysis.

A Clinical Trial on Oral Infection of Preschool Children.

Goals: Over the past twenty years, the existence of oral () infection has been controversial and is still disputed. It proposes that living H. pylori do not exist in the oral cavity.

Clinical Significance of B7-H3 Expression During the Progression of Hepatitis B Virus Infection.

B7-H3, one of the costimulatory members participating in checkpoint pathway, has been shown to be upregulated after hepatitis B virus (HBV) infection. To further explore the clinical significance of dynamic B7-H3 expression during the progression of HBV infection, we systematically investigated the expression pattern of B7-H3 and the correlation of B7-H3 expression with the ratio of T lymphocyte subsets and clinical parameters at different stages in the course of the disease. Flow cytometry and enzyme-linked immunosorbent assay data showed that soluble form of B7-H3 (sB7-H3) was positively correlated with the frequency of T cells in acute hepatitis B (AHB), chronic hepatitis B (CHB), and hepatocellular carcinoma patients with HBV infection (HBV-HCC).

The role of LGR5 and ALDH1A1 in non-small cell lung cancer: Cancer progression and prognosis.

The Leucine rich repeat containing G protein coupled receptor 5 (LGR5), may be a candidate marker of non-small cell lung cancer (NSCLC) cells with stem cell-like properties. Aldehyde dehydrogenase 1A1 (ALDH1A1) is one of NSCLC stem cell markers. To identify the relationship of LGR5 and ALDH1A1 in NSCLC, we analyzed the expression of LGR5 and ALDH1A1 in NSCLC samples, and determined their clinical significance.

[Establishment of a sandwich ELISA for testing serum sB7-H3 and detection of sB7-H3 levels in liver disease].

Aim: To establish quantitative ELISA for soluble sB7-H3 and evaluate its clinical application.

Methods: Two mAbs of mouse anti-human sB7-H3(4H7 and 2E6)established by our lab were used. The mAb 4H7 was used as coating antibody and the 2E6 as a sandwich antibody, which recognized a different epitope and was labeled by biotin.

[Development a method of quantitative assay for enterovirus 71].

Objective: To establish a quantitative assay for enterovirus 71, this can be used in detecting the virus content during vaccine development and production.

Methods: We established the method of quantitative assay for EV71 by using double antibody sandwich ELISA. The sensitivity, accuracy,precision and specificity of the method were evaluated.

[Preparation of a 96-microwell plate DNA diagnostic chip for detection of foodborne bacteria and its application in an incident of food poisoning].

Objective: To develop a 96-microwell plate DNA diagnostic chip for simultaneous detection of 9 major foodborne bacteria.

Methods: Type-specific PCR primers labeled with biotin and oligonucleotide probes were designed according to the conservative genes of 9 major foodborne bacteria Staphylococcus aureus, Salmonella spp., Escherichia coli O157:H7 (Stx1 and Stx2), Shigella spp.

Study of 1-3-2 type piezoelectric composite transducer array.

Based on a new structure of precise location and decoupling between the transducer elements, high frequency underwater transmission transducer arrays with 4 elements and 8 elements serried uniform linear array were studied, using novel 1-3-2 type piezoelectric composite as the sensing material. There are ceramic framework supports in the transverse and longitudinal directions in 1-3-2 type piezoelectric composite structure; the transducer is free from the influence of outside mechanical impact and the environment temperature change. Transducer and array samples have been designed, fabricated and measured.

A study of a 1-3-2 type piezoelectric composite.

A novel 1-3-2 type piezoelectric composite is developed. There are piezoelectric ceramic framework supports at transverse and longitudinal directions in its structure, so it is free from the influence of any outside mechanic impact and environmental temperature change. The sample characteristic is good coherence.

[The assessing tools of the treatment planning in X-knife].

The treatment planning can directly decide the treatment effect of X-knife. This paper presents some qualitative and quantitative tools assessing the treatment planning, mainly they are the isodose curve, dose-wash, volume dose and dose volume histogram.

[The automatic optimization arithmetic of selecting beam arcs in X-knife radiotherapy planning system].

In this paper, two new concepts of DR (Dose Repulsion) and DG (Dose Gravitation) are presented with their calculation formulas. For the problem of selecting beam arcs in x-knife radiotherapy Planning system, a mathematics model of constrained optimization has been built. Furthermore, we have produced a feasible project of automatic selecting optimized beam arcs plan using SA (Simulated Annealing) arithmetic based on the distribution of the fields of DR and DG in the reduced phase space.

[Three-dimensional reconstruction of human organs based on magnetic resonance imaging data].

Objective: To build proper three-dimensional models of human organs for radiation dose calculation.

Method: Human organ models were built by contour reconstruction algorithm conforming to 4 criteria. The contours of the organs were extracted based on the contours of the reconstructed organ surfaces, and the three-dimensional models with smooth surface, exact structure and good visibility were completed by computer graphic techniques such as lighting, smoothing, and normal average.

Registration of serial tissue section images.

OBJECTIVE: This study aims to tackle the problem of image registration during computer-assisted three-dimensional (3D) reconstruction of serial tissue sections. METHODS: We proposed segmentation-counting algorithm for computerized image registration on the basis of joint histogram. This approach utilizes thresholding of the 2 images to be registered, and the criterion function is defined as the counting in a specific region of the joint histogram.

A method for three-dimensional surface reconstruction of volume data field.

Objective: To explore a novel method of three-dimensional (3D) reconstruction based on vector field smoothing, for the purpose of 3D surface reconstruction of DICOM format volume data sets.

Methods: 3D external surface of three sets of volume data, namely craniocerebral volume data, pelvis volume data, and rat embryo volume data, were respectively extracted by Marching Cubes algorithm using small triangle flakes to approach the original 3D structure surfaces. Vector field smoothing was performed on the extracted 3D surfaces.

Elastic registration of medical images through multiquadric method.

Objective: To improve the precision and reliability of elastic registration of the medical images and to simplify the registration process.

Methods: Previous study concerning elastic registration mostly focused on manual selection of the landmarks and then use of adequate interpolating for elastic transformation. The landmarks extraction, however, was prone to error that often showed impact on the registration results, besides the difficulty and time consumption of manual identification of the landmarks.