Publications by authors named "Shu-xia Lv"
[Expression, purification and activity assay of Streptococcus suis serotype 2 cell wall protein SSU1664].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
July 2011
Article Synopsis
- The study aimed to amplify and express the SSU1664 gene from Streptococcus suis in E.coli, and to evaluate the resulting protein's activities.
- The gene was amplified using PCR, cloned into an expression vector, and the protein was purified; its immunogenic properties were assessed using Western blot and ELISA.
- Results indicated that the recombinant protein was immunogenic, with higher levels of anti-SSU1664 antibodies in infected patients, suggesting it could serve as a vaccine candidate and diagnostic marker for early Streptococcus suis infection.
Cloning, expression and preliminary crystallographic analysis of the equine infectious anaemia virus (EIAV) gp45 ectodomain.
Acta Crystallogr Sect F Struct Biol Cryst Commun
April 2011
Like human immunodeficiency virus (HIV), equine infectious anaemia virus (EIAV) belongs to the lentivirus genus. The first successful lentiviral vaccine was developed for EIAV. Thus, EIAV may serve as a valuable model for HIV vaccine research.
View Article and Find Full Text PDF[Purification and biological activities analysis of streptococcus suis Serotype 2 suilysin].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
April 2011
Aim: To explore the purification methods of wild-type and recombinant suilysin and to evaluate their biological activities.
Methods: Wild-type suilysin was purified by ammonium sulfate precipitation, anion-exchange chromatography and hydrophobic chromatography in turn, while recombinant suilysin was first refolded and purified by immobilized metal ion affinity chromatography, and further purified by Thiopropyl Sepharose 6B. The biological activities were evaluated by hemolysis test, cytotoxicity assay.
Constructing eukaryotic expressing vector of pMT/Bip/V5-His A-HIV gp120 and transfecting S2 cells to establish stable gp120-expressing S2 cell line. The gp120 gene of HIV CRF07-BC epidemic in China was amplified by polymerase chain reaction from the recombinant vector PRSV-gp120 and confirmed by DNA sequence analysis. The DNA fragment of HIV gp120 was digested with the restriction endonucleases NcoI and XhoI, then inserted into eukaryotic expressing vector pMT/BiP/V5-His A.
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