Regulatory effect of isovitexin on MAPK/NF-B signal in mice with acute ulcerative colitis.

The aim of this study was to investigate the anti-inflammatory effects and mechanism of isovitexin on ulcerative colitis mice and RAW264.7 cells. The results showed that isovitexin had strong antioxidant and anti-inflammatory effects, and could restore intestinal barrier integrity ( < 0.

New Bisabosquals from sp. PH30583 Elicited on Solid Media.

sp. PH30583 cultured in liquid medium only led to one structure type of novel isochroman dimers. Using the one strain-many compounds strategy, the reinvestigation of the metabolites from sp.

Benzopyran derivatives from endophytic JC-15 in and their bioactivities.

Five new benzopyran derivatives (-) and a new natural product () were isolated from endophytic in and determined as (R)-2,3-dihydro-2,5-dihydroxy-2-methylchromen-4-one (), (2R, 4S)-2,3-dihydro-2-methyl-benzopyran-4,5-diol (), (R)-3-methoxyl-1-(2,6-dihydroxy phenyl)-butan-1-one (), 7-O-α-d-ribosyl-5-hydroxy-2-methyl-4H-chromen-4-one (), 7-O-α-d-ribosyl-2,3-dihydro-5-hydroxy-2-methyl-chromen-4-one (), daldinium A (). These compounds were evaluated for their antimicrobial activity, anti-acetylcholinesterase, nitric oxide inhibition, anticoagulant, photodynamic antimicrobial activities and glucose uptake of adipocytes. Some compounds showed photoactive antimicrobial activities and glucose uptake stimulating activities.

[Study on the incidence of beta-Thalassemia and genotypes among children under 7 year-olds in Nanning, Liuzhou and Baise areas, Guangxi province].

Objective: To conduct research of beta-Thalassemia incidence and genotypes on children below 7 years of age in Nanning, Liuzhou and Baise areas, Guangxi province.

Methods: A total of 2261 children aged below 7 in Nanning, Liuzhou and Baise areas were studied. Venous blood was detected by routine blood test, hemoglobin analysis and beta-Thalassemia genotyping.

[Prevalence of hypertension in the Guangxi Bai Ku Yao populations].

Objective: To observe the prevalence of hypertension and associated risk factors in the Guangxi Bai Ku Yao populations.

Methods: A total of 1170 subjects of Bai Ku Yao aged 15 and over were surveyed by a stratified randomized cluster sampling. Blood pressure, body height, weight, waist circumference, serum lipid and apolipoprotein levels were measured, and body mass index (BMI) were calculated, matched 1173 subjects of Han Chinese from the same region served as control.

[Non-invasive prenatal genetic diagnosis of beta-thalassaemia using single fetal nucleated erythrocyte from maternal blood].

Objective: To investigate non-invasive prenatal genetic diagnosis of beta-thalassaemia using a single fetal nucleated erythrocyte (NRBC) from maternal blood by comparing with the genotype obtained from chorionic villus or amniocytes, and to evaluate the diagnostic results in reliability and feasibility of this method.

Methods: Maternal blood samples were obtained from 28 pregnant women at risk of beta thalassaemia during 9 - 34 weeks of gestation. NRBCs in maternal blood were enriched by single density gradient separation, stained with benzidine, and then collected by micromanipulation individually.

[Prenatal gene diagnosis of paternally inherited alpha-thalassemia by detecting fetal DNA in maternal plasma].

Objective: To evaluate the value of diagnosis of alpha-thalassemia by analyzing fetal DNA in maternal plasma.

Methods: Ten families were screened, the husbands being alpha-thalassemia Southeast Asia deletion (SEA alpha-thalassemia-1) heterozygotes and the pregnant women being alpha-thalassemia-2 heterozygotes. Fluorescent polymerase chain reaction (PCR) and gene scanning were used to detect the paternally inherited genotypes of SEA alpha-thalassemia-1 gene mutation and short tandem repeats (STRs) in the maternal plasma fetal DNA.

[Prenatal diagnosis of Thailand deletion of alpha-thalassemia 1].

Objective: To conduct prenatal diagnosis on the couples carrying Thailand deletion (--THAI) alpha-thalassemia 1 and at high risk of having fetus with alpha-thalassemia.

Methods: Genotypes of couples and fetuses were analyzed by PCR and DNA sequencing.

Results: Four pregnant women were patients with Hb H diseases of --THAI compounding with alpha-thalassemia 2, while their husbands were heterozygote of the Southeast Asian type alpha-thalassemia 1 (--SEA).

[Identification of fetal nucleated erythrocytes in maternal blood using short tandem repeat typing after primer extension preamplification].

Objective: To develop a method for identifying fetal nucleated erythrocytes (NRBCs) in maternal blood.

Methods: NRBCs in maternal blood were detected by benzidine staining and collected by micromanipulation. After primer extension preamplification (PEP) of the entire genome from a single NRBC, short tandem repeat (STR) genotype was analysed after further amplification of this gene.

[Alpha 2 codon 30 deletion (deltaGAG) causing non-deletional hemoglobin H disease in Guangxi province].

Objective: To analyze genotypic profiles and understand the relationship between the genotype and phenotype of Hb H disease in Guangxi province.

Methods: Hematologic and Hb analyses on the cases were performed to detect their alpha thalassemia genotypes using PCR method and DNA sequencing.

Results: An unusual case was identified in one of the 298 patients with Hb H disease diagnosed in the First Affiliated Hospital of Guangxi Medical University from October 2002 to November 2003.

[Homozygous hemoglobin constant spring in Guangxi province].

Objective: To analyze the relationship between genotype and phenotype of homozygous hemoglobin Constant Spring (Hb CS) in Guangxi province, and to explore the reasons of missed diagnosis and the methods for screening and diagnosing.

Methods: Screening Hb CS by acetate fibrous membrane electrophoresis with benzidine staining. Gene mutation of homozygous Hb CS by polymerase chain reaction (PCR) and DNA sequencing.

[Eosinophils apoptosis in asthmatic children].

Unlabelled: Prominent eosinophil airway inflammation is important in the pathogenesis of asthma. There is increasing evidence that the disorder of eosinophil apoptosis contributes to the mechanism. But most of the studies have been done in vitro or on animal models, very few were done among the adult asthmatics in vivo.