[Effects of different altitudes on cardiac hemodynamics and electrocardiogram of healthy male adults].

Objective: To detect the changes of cardiovascular system at different altitudes, so as to prevent and predict the susceptibility to acute mountain sickness.

Methods: The test was performed with noninvasive cardiovascular monitor and electrocardiogram in healthy male Chinese members of the 25th and 26th expeditions to the antarctic kunlun station at different altitudes (40 m, 3 650 m and 4 300 m).

Results: Compared with 40 m, from 3 650 m to 4 300 m, heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), systemic vascular resistance (SVR), systemic vascular resistance index (SVRI) significantly increased (P < 0.

[The relationship between body fat percentage, waist-hip ratio and pulmonary ventilatory function among the adult females in Heilongjiang, in 2008].

Objective: To examine the effect of body fat mass and fat distribution on pulmonary ventilatory function among the adult females.

Methods: Based on the multistage cluster sampling principal, we selected 935 healthy adult females with ages of 19-81 years old in Heilongjiang province to conduct the study. Every 10-years old as a age group.

[Relation between fat mass, fat free mass and ventilatory function in children and adolescents].

The aim of the present study was to evaluate the relation between fat mass (FM), fat free mass (FFM) and ventilatory function in children and adolescents. 1 174 healthy children and adolescents (583 males and 591 females) aged 10-18 years were selected from Heilongjiang Province through random sampling by means of questionnaire and physical examination, and measured for height, weight, waist to hip ratio (WHR), FM, FFM and ventilatory function. The data were analyzed by means of independent-samples t test, Pearson correlation analysis and multi-factors regression analysis.

[Fat mass and fat free mass on ventilatory function in adults].

This study is designed to probe for the effects of fat mass (FM) and fat free mass (FFM) on ventilatory function in adults. 1 307 healthy adults (372 males and 935 females) were selected from some localities of Heilongjiang province through random sampling by means of questionnaire and physical examination and measured for height, weight, waist to hip ratio (WHR), FM, FFM and ventilatory function. The data were analyzed by means of Pearson correlation analysis, independent-samples t test and multi-factors regression analysis.

[The relationship between percentage body fat, waist-hip ratio and cardiovascular function in the adult females of Heilongjiang province].

Objective: To analyze the relationship between body fat mass and distribution and cardiovascular function in the adult females of Heilongjiang province.

Methods: Based on the statistic variable random sampling principal, we selected 1903 healthy adult females with ages of 18 - 70 years old in Heilongjiang province to conduct the study. The height, body weight, waist, chest measurement and waist-hip ratio (WHR) were measured.

Reference data for quantitative ultrasound values of calcaneus in 2927 healthy Chinese men.

Quantitative ultrasound (QUS) is a bone densitometry technique that is rapidly gaining popularity for the assessment of bone status. However, few QUS data are available for men, especially in China. In the present study, a random stratified sample of 2927 Chinese men (10-90 years) was recruited, and bone status was established using measurements by Achilles ultrasonometer.

Crucial roles of GATA-2 and SP1 in adrenomedullin-affected expression of tissue factor pathway inhibitor in human umbilical vein endothelial cells exposed to lipopolysaccharide.

Adrenomedullin (ADM) is a vasodilator peptide that has a variety of effects, including antithrombotic activities and resistant roles to lipopolysaccharide (LPS)-induced septic shock. During sepsis, LPS triggers the development of disseminated intravascular coagulation (DIC) via the tissue factor-dependent pathway of coagulation. It is unknown whether the antithrombotic activities of ADM contribute to its resistance to sepsis.

[Ultrasonic bone mineral density of calcaneus in 1 816 healthy subjects in Guangxi autonomous region].

Objective: To study the relationship between ultrasonic bone mineral density (BMD) of calcaneus and age, height, and body weight, and to establish the normal reference value for stiffness index (SI) of healthy subjects in Guangxi autonomous region and provide scientific foundation for the diagnosis and prevention of osteoporosis.

Methods: SI of calcaneus in 1 816 healthy adults aged 11-90 years (775 men, 1 041 women) were measured with quantitative ultrasound. According to their ages, all the men and women were divided into 13 groups by 5-year age group.

[Bone density of calcaneus of 2769 healthy persons in Zhejiang province].

Objective: To provide a scientific basis for the diagnosis and prevention of osteoporosis in Chinese populations.

Methods: The stiffness index (STI) of the calcaneus was measured with the Achilles Express ultrasound apparatus made in USA among 2769 healthy persons, 1262 males and 1507 females, aged 10 to over 60, in Zhejiang province. The relationships between the STI and sex and age were analyzed.

Effects of 17beta-estradiol and phytoestrogen alpha-zearalanol on tissue factor in plasma of ovariectomized rats and HUVECs.

In the present study, the effects of 17beta-estradiol (E2) and phytoestrogen alpha-zearalanol (ZAL) on tissue factor (TF) in plasma of ovariectomized (OVX) rats and human umbilical vein endothelial cells (HUVECs) were investigated. OVX rats were injected intramuscularly twice a week with E2 (1mg/kg) or ZAL (1mg/kg) for five weeks. The concentrations of plasma E2 were measured by radioimmunoassay.

[Analysis of the levels of serum total calcium and inorganic phosphate of 2,342 children in Beijing].

Objective: To study the changes of serum total calcium (Ca) and inorganic phosphate (P) levels during children growth and related factors.

Methods: Using a stratified-multi-steps-cluster sampling method, we selected 2,342 healthy children aged 10-18 years from urban and suburban areas of Beijing from September 2001 to December 2001 to test the levels of serum Ca and P. Their meal status was also surveyed to analyse the correlation of the leves of serum Ca and P with sex, age, height, weight, and diet on statistic basis.

[Effects of TNF-alpha or mmLDL on PAI-1 expression in human umbilical vein endothelial cells].

Objective: To demonstrate the changes of PAI-1 activity and mRNA expressions induced by tumor necrosis factor alpha (TNF-alpha) or minimally modified low-density lipoprotein (mmLDL) in human umbilical vein endothelial cells (HUVECs).

Methods: The PAI-1 activity in HUVECs culture medium was measured by chromogenic assay. The PAI-1 mRNA expressions were determined by Northern blot.

[Effect of AP-1 on expression of plasminogen activator inhibitor-1 in human vascular endothelial cells].

Objective: To study the effect of nuclear transcription factor AP-1 on tumor necrosis factor alpha (TNF-alpha) or minimal modified low density lipoprotein (mmLDL)-induced expression of plasminogen activator inhibitor-1 (PAI-1) in human vascular endothelial cells.

Methods: Using gene recombination techniques, four luciferase reporter gene plasmids containing different length of human PAI-1 gene promoter were constructed. Through the transient transfection analysis, the roles of AP-1 element (from -823 bp to -553 bp) in PAI-1 promoter have been determined.