[Gene editing for the treatment of primary immunodeficiency disease].

Gene editing is an advanced technique based on artificial nucleases and can precisely modify genome sequences. It has shown great application prospects in the field of medicine and has provided a new precision therapy for diseases. Primary immunodeficiency disease is a group of diseases caused by single gene mutation and characterized by recurrent and refractory infections, with an extremely high mortality rate.

Indocyanine Green Uptake and Periodic Acid-Schiff Staining Method for Function Detection of Liver Cells are Affected by Different Cell Confluence.

Unlabelled: Hepatic stem cell transplantation has been demonstrated as an effective alternative therapy for the end-stage liver failure patients. Therefore, the functional detection of hepatic stem cell is essentially required. The present study confirmed that adenovirus BMP9 (Ad-BMP9) could increase the ALB-Gluc activity of HP14-19 hepatic progenitor cells, the expression of specific hepatic markers ALB, TAT, UGT1A were up-regulated while the hepatic stem cell markers DLK, AFP were down-regulated, and the number of positive Periodic acid-Schiff (PAS) stained cells were significantly higher than those in control group.

[Twist regulates proliferation, migration and invasion of osteosarcoma cells in vitro].

Objective: To investigate the role of Twist in regulating the proliferation, migration, and invasion of osteosarcoma cells with different levels of malignancy.

Methods: The baseline expressions of Twist in 3 different osteosarcoma cell lines (143B, MG63 and TE85) were detected using real-time PCR and Western blotting. The cells were infected with the recombinant adenoviruses Ad-Twist or Ad-siTwist for Twist overexpression or knockdown, respectively, and the cell growth curves were drawn to assess the cell proliferation.

[Changes in autophagy during maturation and differentiation of Hepa1-6 cells induced by all-trans retinoic acid].

Objective: To investigate the effects of different concentrations of all-trans retinoic acid (ATRA) on the maturation, differentiation and autophagy of Hepa1-6 cells.

Monthod: Hepa1-6 cells were treated with 0.1, 1, and 10 µmol/L ATRA, and the changes in the expressions of hepatic specific markers were detected using real-time PCR and Western blotting.

Trapping the lead ion in multi-components aqueous solution by natural clinoptilolite.

To study whether natural clinoptilolite could selectively capture Pb(2+) ion in the aqueous solution containing salt and glycine as well as nitrosamines, the natural zeolite was utilized in the complex solution with pH value of 1.2 in comparison with other porous materials such as zeolite NaZSM-5 and activated carbon. Clinoptilolite exhibited the highest capability in adsorbing Pb(2+) ion in the solution at 37 degrees C, achieving the capacity of 7 mg/g, two times more than that by other zeolites and six times over that by activated carbon, and the adsorption equilibrium could be achieved within 2h.