Protein expression profile of human renal mesangial cells under high glucose.

Background: To understand the spectrum of proteins affected by diabetic nephropathy and to characterize the molecular functions and biological processes they control, the protein expression profile of human renal mesangial cells (HMCs) under high glucose was analyzed.

Methods: HMCs were divided into a high glucose-cultured group (30 mmol/l) and a normal glucose-cultured group (5 mmol/l). The total proteins of the two groups were separated and analyzed by two-dimensional difference gel electrophoresis (DIGE).

Association of cyclooxygenase-2 expression with Hp-cagA infection in gastric cancer.

Aim: To observe the expression of cyclooxygenase-2 (COX-2) and to investigate the association between COX-2 expression and infection with cytotoxic-associated gene A (cagA) positive strain Helicobacter pylori (Hp) in human gastric cancer, and subsequently to provide fresh ideas for the early prevention of gastric cancer.

Methods: 32 Specimens of gastric cancer and corresponding adjacent normal gastric mucosa were obtained from patients who had undergone surgical operations of gastric cancer. All the samples including 1 case of stomach malignant lymphoma and 31 cases of gastric adenocarcinoma were confirmed by pathology diagnosis.