A drug delivery system of siRNA nanoparticles loaded by mesenchymal stem cells on choroidal neovascularization.

To assess mesenchymal stem cells (MSCs) as carriers for  siRNA-loaded nanoparticles (NPs) for targeted therapy of experimental choroidal neovascularization (CNV). A poly (lactic-co-glycolic acid) (PLGA)-core/lipid-shell hybrid NP was designed. The transfection efficacy of MSCs with the hybrid NPs was assessed.

Enlarged perivascular spaces, neuroinflammation and neurological dysfunction in NMOSD patients.

Background And Objectives: Cerebrospinal fluid (CSF) and interstitial fluid exchange along a brain-wide network of perivascular spaces (PVS) termed the 'glymphatic system'. The aquaporin-4 (AQP4) water channels abundantly expressed on astrocytic endfeet play a key role in the CSF circulation in the glymphatic system. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory demyelinating autoimmune disease of the central nervous system (CNS) featured with a specific autoantibody directed against AQP4 in most of patients.

Pax6 regulates proliferation and apoptosis of human retinoblastoma cells.

Purpose: To assess whether the Pax6 gene is involved in the development of retinoblastoma.

Methods: Three human retinoblastoma cell cultures were transfected with human Pax6 specific double-stranded, small interfering siRNA molecules RH-1 and RH-2. In addition, untreated control groups and negative control groups (CT groups) transfected with siRNA without homology to the human genome were formed for all three cell culture lines.

Constitutively active Akt contributes to vincristine resistance in human retinoblastoma cells.

Akt mediates survival signals which preserve various cancer cells from apoptosis. Its correlation with chemoresistance of cancer cells has attracted much attention. But how Akt affects vincristine-induced cell death in retinoblastoma is unknown.