[Effect of Shh and BM-MSC Synergism on the Proliferation of Hematopoietic Stem Cells].

Objective: To study the effect and mechanism of shh and mesenchymal stem cell（MSC）synergism on the proliferation of hematopoietic stem cells in noninvasive co-culture system in vitro.

Methods: The mesenchymal stem cells were cultured in vitro，CD34 cells were sorted by mini MACS magnetic bead separator，flow cytometry was used to identify the purity of 2 cells. CD34 cells and MSCs were seeded to upper and low of transwell respecibely for non-contact coculture，and add exogenous shh protein for intervenece.

[Influencing Factors of Nosocomial Infection after Chemotherapy in Adult Patients with Acute Myeloid Leukemia and Its Control Strategy].

Objective: To investigate the influencing factors of nosocomial infection in adult patients with acute myeloid leukemia (AML) and its control strategies.

Methods: The clinical data of 109 patients with AML treated in our hospital from June 2014 to June 2017 were retrospectively analyzed. The clinical factors were analyzed retrospectively, and the influencing factors of infection after chemotherapy were explored.