Enhanced Signal and Quantitative Detection of Anti-Interferon-Gamma Antibody by Using a Nanometer Biolinker.

For rapid screening and quantification of an antisera antibody, a nanometer bithiophene-based conductive biolinker can enhanced signal performance and can be used to verify the interaction of an anti-IFN-γ antibody with an IFN-γ protein. The experimental measurements take a generic approach which takes advantage of the functionality of thiophene-based linkers for biosensors. Effects associated with using bithiophene as a biolinker for surface plasmon resonance (SPR) spectroscopy are examined in this paper.

Ellipsometric surface plasmon resonance.

We develop a new multifunctional optical biochip system that integrates an ellipsometer with a surface plasmon resonance (SPR) feature. This newly developed biochip biosensor, which we call ESPR for an ellipsometric SPR, provides us with a system to retrieve detailed information such as the optical properties of immobilized biomolecular monolayers, surface concentration variations of biomedical reactions, and kinetic affinity between biomolecules required for further biotech analysis. Our ESPR can also serve as both a research and development tool and a manufacturing tool for various biomedical applications.

Phase-shifting algorithms for electronic speckle pattern interferometry.

A set of innovative phase-shifting algorithms developed to facilitate metrology based on electronic speckle pattern interferometry (ESPI) are presented. The theory of a phase-shifting algorithm, called a (5,1) algorithm, that takes five phase-shifted intensity maps before a specimen is deformed and one intensity map after a specimen is deformed is presented first. Because a high-speed camera can be used to record the dynamic image of the specimen, this newly developed algorithm has the potential to retain the phase-shifting capability for ESPI in dynamic measurements.