Changes in Nitric Oxide Releases of the Contralateral Acupoint during and after Laser Acupuncture at Bilateral Same-Name Acupoints in Human.

Objective: The purpose of the study was to examine the effects of laser acupuncture (LA) at right Neiguan (RPC6)/left Neiguan (LPC6) acupoints on the releases of nitric oxide (NO) in the treated and contralateral/nontreated PC6, compared to the nonacupoint control area.

Methods: 24 mW LA at RPC6, LPC6, and nonacupoint in 22 healthy subjects for 40 min: sterilized dialysis tube was taped to the nontreated PC6/nonacupoint during the treatment and immediately taped to the treated and nontreated PC6/nonacupoint after LA removal. NO-scavenging compound was injected into the tube for 40 min to absorb the molecular which was tested by spectrophotometry in a blinded fashion.

Effects of different-intensity laser acupuncture at two adjacent same-meridian acupoints on nitric oxide and soluble guanylate cyclase releases in human.

Objectives: The aim of this study was to detect the influences of LA at nonacupoint and two adjacent acupoints of pericardium meridian on the releases of NO and sGC in 20 healthy subjects.

Methods: Different intensities (12, 24, 48 mW) of infrared laser were used for irradiating Jianshi (PC5), Ximen (PC4) acupoints and nonacupoint for 20, 40 minutes, respectively. Semi-circular tubes were taped to the skin surface and filled with NO-scavenging compound for 20 minutes to capture NO and sGC, which were measured using spectrophotometry in a blinded fashion.

Mechanism study of laser cochleostomy-induced early hearing loss in a rat model.

Hearing loss following laser-assisted ear surgery has been reported. However, the mechanism responsible for the hearing loss remains largely speculative. The aim of this study was to investigate the correlation between laser-induced hearing loss and changes in the number of hair cell ribbon synapses and ultrastructure in the cochlea.

[Effects of laser-assisted cochleostomy on inner hair cell ribbon synapse in rats].

Objective: To investigate the effects on ribbon synapse of inner hair cells after superpulsed CO2 laser-assisted cochleostomy in SD rats.

Methods: Eighteen SD rats were randomly divided into laser-assisted surgery groups (2 W group and 5 W group), sham-operated group and control group. Ten of those were performed a cochleostomy using superpulsed CO2 laser with a corresponding power.

[Comparative study of time-correlated temperature and back-scattered light intensity for human Hegu acupoint and non-acupoint tissue irradiated by near-infrared laser].

Characteristics and differences of temperature and back-scattered light intensity in different depths of 0.2, 0.4, 0.

[Low level laser irradiation in the visible spectra induces HeLa cells proliferation].

The aim of this in vitro study was to evaluate the effects of low level laser irradiation on the proliferation of HeLa cells using 405 nm diode laser, 514 nm argon laser, 633 nm He-Ne laser, or 785 nm diode laser, The cells were seeded on 96-well microplates for 24 h in 5% fetal bovine serum containing medium, then irradiated with the laser at dose of 100 and 1 000 J x m(-2), respectively. At the time point of 24, 48, 72 h after irradiation, cell viability was assessed by MTT assay. The results show that 405, 633 and 785 nm laser irradiation induces wavelength-dependent and time-dependent proliferation.

[In vivo reflectance spectroscopy study of different clearing agents on human skin optical clearing].

The changes of skin tissue reflectance spectroscopy before and after being treated with the optical clearing agents of three different types of optical clearing within the wavelength rang of 400-1 000 nm, and the degree of changes in reflectance spectroscopy of each group skin during 0-60 min at 580 nm in vivo were real-time dynamically researched. The reflectance spectroscopy of skin tissue before and after being dealt by the optical clearing agents of glycerol, glucose and propylene glycol was measured using a USB-4000 fiber spectrophotometer at 0, 10, 20, 30, 40, 50 and 60 min. The results showed that the reflectance spectral intensity was distinctly decreased, but the reflectance was significantly increased gradually with the time prolonged.

[Reflectance spectroscopy study of low-frequency ultrasound and glycerol on skin optical clearing].

In order to find a non-invasive way to improve the efficacy of skin optical clearing with topically applied optical clearing agents, the authors researched the changes of reflectance spectroscopy of skin tissues before and after being dealt by low-frequency ultrasound and osmotic active chemical agents within the wavelength range of 400-860 nm, and the degree of changes in reflectance spectroscopy of each group skin during 0-15 min and 15-30 min at 580 nm. The measurements were performed using a AvaSpec-2048 optical fiber spectrometer with integrating-sphere setup. The results of measurements showed that there were a few changes of the reflectance spectroscopy of skin tissues in the control group (Group 1) (The skin tissue was dealt with nothing.

[Comparative study of reflectance spectroscopy of human laogong acupoint and non-acupoint tissues irradiated by near-infrared laser].

Characteristics and differences of reflectance spectroscopy of human Laogong acupoint and non-acupoint tissues before and after irradiation by different power of laser were studied in the spectral range from 400 to 1 000 nm. A wavelength 808 nm semiconductor laser was used for irradiation at the power of 20, 50 and 100 mW for ten minutes. Reflectance spectra of human Laogong acupoint and non-acupoint tissues were measured by using an AvaSpec-2048 optical fiber spectroscopy with an integrating sphere attachment.

[Spectral characteristics of normal breast samples in the 350-850 nm wavelength range].

Spectral characteristics of normal female breast samples in the 350-850 nm wavelength range were measured using a UV/Vis/NIR spectrophotometer system with integrating sphere attachment for measuring the diffuse reflectance and transmittance. The optical properties of normal breast tissue in vitro were obtained by the inverse adding doubling method. And then the optical penetration depths in this spectral range were analyzed based on the principle of tissue optics.

[Gastric cancer detection using kubelka-Munk spectral function of DNA and protein absorption bands].

Differential diagnosis for epithelial tissues of normal human gastric, undifferentiation gastric adenocarcinoma, gastric squamous cell carcinomas, and poorly differentiated gastric adenocarcinoma were studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 250 to 650 nm, pathological changes of gastric epithelial tissues induced that there were significant differences in the averaged value of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log[f(r infinity)] of the DNA absorption bands at 260 nm between epithelial tissues of normal human stomach and human undifferentiation gastric cancer, between epithelial tissues of normal human stomach and human gastric squamous cell carcinomas, and between epithelial tissues of normal human stomach and human poorly differentiated cancer.

[Colon adenoma detection using Kubelka-Munk spectral function of DNA and protein bands].

Differential diagnosis of human colon adenoma was studied using the Kubelka-Munk spectral function of the DNA and protein absorption bands at 260 and 280 nm in vitro. Diffuse reflectance spectra of tissue were measured using a spectrophotometer with an integrating sphere attachment. The results of measurement showed that for the spectral range from 590 to 1 064 nm pathological changes of colon epithelial tissues were induced so that there were significant differences in the averaged values of the Kubelka-Munk function f(r infinity) and logarithmic Kubelka-Munk function log [f(r infinity)] of the DNA absorption bands at 260 nm between normal and adenomatous colon epithelial tissues, and the differences were 218% (p < 0.

[Measurement of nasopharyngeal carcinoma tissue ex vivo by Raman spectroscopy].

Raman spectroscopy has shown its potential and advantages in detecting molecular changes associated with tissue pathology, which makes it possible to diagnose with optical methods non-invasively and real-time. A compact and rapid near-infrared (NIR)Raman system was developed using 785 nm diode laser, volume phase technology (VPT)holographic grating system and NIR intensified charge-coupled device (CCD)with a specially designed Raman fibre probe which can effectively reduce the interference of fluorescence and Rayleigh scattering, maximize the ability of Raman collection as well as correct the image aberration of a planar grating diffraction. Adopting this method, signal-to-noise ratio has been greatly improved and human tissue signals can be acquired in a short time.

Differences in optical transport properties between human meridian and non-meridian.

This study is to present a new scheme for the detection of human meridian system non-invasively. The optical transport properties along the pericardium meridian and a non-meridian path about 1 cm away from the meridian were measured non- invasively on 20 healthy people in vivo. 633 nm, 658 nm and 785 nm red lasers were used for irradiation, and the diffuse light emittances at different points on meridian and non-meridian directions were collected respectively and compared.

Appearance of human meridian-like structure and acupoints and its time correlation by infrared thermal imaging.

The meridians and acupoints of human bodies at natural condition are investigated among 30 healthy volunteers by infrared thermal imaging technique. The results give clear evidence of the existence of infrared radiant tracks along human meridian courses. The time dependent evolution of the infrared radiant track is observed for the first time.

[The impact of blood content in skin tissue on skin spectra].

Based on the analysis of skin structure, and the production mechanism of fluorescence and reflectance spectra, six-layer optical models for skin of different blood content in both upper blood plexus and deep blood plexus were developed, and Monte Carlo simulation was made. The result shows that (1) Both fluorescence and reflectance spectra could reflect the change of blood content in skin tissue; (2) The impact of blood content in upper blood plexus on skin spectra intensity is large, while the impact of blood content in deep blood plexus on skin spectra intensity is small; (3) Fluorescence and reflectance spectra could be used to detect or analyze change of blood content in skin tissue, especially to test the treatment and tune of dermatosis with plexus or blood pathological changes in upper dermis.

[Comparison of light attenuation characteristics and optical penetration depths between native and coagulated human liver tissues].

A double-integrating-spheres and IAD method were used to study the differences in the optical penetration depths (OPDs) and light attenuation (LA) native and coagulated human liver tumors and liver tissues at the wavelengths of 680, 720, 780, 810, 850 and 890 nm of Ti: Sapphire laser. The results of measurement showed that the OPDs for native and coagulated human liver tumors and liver tissues at six different wavelengths obviously increase with increasing laser wavelength, the OPDs of coagulated human liver tumors and liver tissues at six different wavelengths were significantly smaller than that of native human liver tumors and liver tissues at the same wavelength respectively (P<0.05), and the OPDs of native and coagulated human liver tumors at six different wavelengths were significantly bigger than that of native and coagulated human liver tissues at the same wavelength respectively (P<0.

[Fluorescence spectral characteristics of human blood and its endogenous fluorophores].

The three-dimensional fluorescence spectra and excitation-emission matrix of human blood were measured, and an attempt was made to exploit the endogenous fluorophores of major peaks in the UV and visible. The result indicates that the absorption peaks of human blood appear at 274, 345, 415, 541 and 576 nm. Based on the analysis of fluorescence excitation-emission matrix, the major emission peaks of human blood occur at excitation-emission wavelength pairs of 260-630, 280-340, 340-460 and 450-520 nm, which are attributed to endogenous porphyrins, tryptophan, reduced nicotinamide adenine dinucleotide (NADH) or reduced nicotinamide adenine dinucleotide phosphate (NADPH), and flavin adenine dinucleotide (FAD), respectively.

[Fourier transform infrared and near-infrared Raman spectra of human red blood cell].

The human red blood cell was studied by Fourier transform infrared and near-infrared Raman spectroscopy.The assignment of the characteristic groups of human red blood cell structure was basically confirmed. At the same time, the authors observed that the intensity of different Raman shift signal has different varying law when the power of laser is changing.

Autofluorescence excitation-emission matrices for diagnosis of colonic cancer.

Aim: To investigate the autofluorescence spectroscopic differences in normal and adenomatous colonic tissues and to determine the optimal excitation wavelengths for subsequent study and clinical application.

Methods: Normal and adenomatous colonic tissues were obtained from patients during surgery. A FL/FS920 combined TCSPC spectrofluorimeter and a lifetime spectrometer system were used for fluorescence measurement.

[Time-resolved fluorescence studies of hematoporphyrin monomethyl ether for photodynamic diagnosis].

Time-resolved fluorescence spectroscopic measurements of second-generation new photosensitizer of hematoporphyrin monomethyl ether (HMME) were carried out in the physiological saline and different human serums by picosecond fluorescence lifetime spectrometer, respectively. The objective was to assess the fluorescence lifetime of HMME as a new enhanced contrast parameter between normal and malignant tissues for the photodynamic diagnosis of various cancers. The influence of emission wavelength and photosensitizer concentration, on the lifetime of HMME was also presented.

[Spectral properties of new photosensitizers for photodynamic diagnosis and therapy].

The spectral properties of new photosensitizer ZnPcS2P2, PsD-007 and HMME, as well as traditional photosensitizer HpD have been studied by comparing their spectra in physiological saline and in physiological saline with 10 percent serum. Experimental results show that the maximum absorption peaks for PsD-007, HMME and HpD in the physiological saline with 10 percent serum appear at 400 nm in the soret region, while at 670 nm for ZnPcS2P2. The fluorescence excitation spectra closely resemble the absorption spectra.