[Occurrence Characteristics of Microplastics and Metal Elements in the Surface Water of Huangpu River and Their Associations with Metal Resistance Genes].

Urban rivers have been regarded as the "hotspots" for microplastic (MPs) and metal contamination as they play important roles in pollution migration. However, as important sinks and sources of resistance genes, there has been little to no research investigating the associations between MPs, metal contaminations, and metal resistance genes (MRGs). Ten water samples were collected from the Huangpu River ; along with metal elements, MPs characteristics analyzed.

Reparative effects of chronic intermittent hypobaric hypoxia pre‑treatment on intervertebral disc degeneration in rats.

Previous studies have indicated that chronic intermittent hypobaric hypoxia (CIHH) preconditioning can inhibit TNF‑α and other related inflammatory cytokines and exerts protective effect on intervertebral disc degeneration disease (IDD) in rats; however, the mechanism is still unclear. The present study aimed to explore the repair mechanisms of CIHH on IDD in rats. In the experiment, 48 adult Sprague‑Dawley rats were selected and randomly divided into an experimental group (CIHH‑IDD), a degenerative group (IDD) and a control group (CON).

[Salt stress tolerance of cucumber-grafted rootstocks].

Taking 4 different Cucurbita maxima x C. moschata rootstocks for cucumber (Cucumis sativus) as test materials, a solution culture experiment was conducted to study their growth and antioxidative enzyme activities under the stresses of Ca(NO3)2 and NaCl, with the salt stress tolerance of the rootstocks evaluated by subordinate function. At 30 mmol x L(-1) of Ca (NO3)2 or 45 mmol x L(-1) of NaCl, the growth of the rootstock seedlings was improved; but at 60 and 120 mmol x L(-1) of Ca(NO3)2 or 90 and 180 mmol x L(-1) of NaCl, the growth and the antioxidative systems of the seedlings were inhibited, and the salt injury index of 'Qingzhen No.

[Study on the immune response of the therapeutic multi-epitope gene vaccine of hepatitis B virus in mice].

Aim: To study the immunogenicity of the therapeutic multi-epitope gene vaccine of Hepatitis B virus.

Methods: Multi-epitope gene of Hepatitis B virus was cloned into prokaryon expression vector pBAD/gIIIA to express a non-fusional antigen B-BPT and BALB/c mice were immunized with the antigen. Cytotoxic T-lymphocyte(CTL) was determined with CytoTox96 kit which quantitatively measures the lactate dehydrogenase(LDH) that is released on cell lysis.

[Effect of adjusting the immunosuppressants on the prognosis of the infection in the patients with liver transplantation].

Objective: To explore the method of adjusting the immunosuppressants in serious infection after liver transplantation.

Methods: With reference to sepsis-related organ failure assessment (SOFA), 2005.1-2007.

[Construction and expression of non-fusional expression vector of the multi-epitope gene of hepatitis B virus].

Aim: To study the cloning, expression and antigen of therapeutic multi-epitope gene of hepatitis B virus.

Methods: The multi-epitope gene of hepatitis B virus(BPT) was designed, synthesized and cloned into the prokaryotic expression vector pBAD/gIIIA. Then it was transformed into E.

[Impact of adaptive positive end expiratory pressure and mechanical ventilation on hemodynamics and oxygen kinetics in post-liver transplantation patients].

Objective: To determine the impact of adaptive positive end expiratory pressure (PEEP) and mechanical ventilation on hemodynamics and oxygen kinetics in post-liver transplantation patients.

Methods: The study included 11 patients who accepted mechanical ventilation after piggyback liver transplantation. Swan-Ganz catheter and radial artery catheter were used to monitor the cardiac output (CO), mean pulmonary arterial pressure (MPAP), mean arterial blood pressure (MABP) and central venous pressure (CVP) and airway pressure.

[Effect of HBsAg pulsed dendritic cells on the functions of cytokine-induced killer cells].

Aim: To explore the effects of HBsAg-pulsed dendritic cells (DCs) on the proliferation and killing functions of cytokine-induced killer (CIK) cells.

Methods: The peripheral blood mononuclear cells (PBMCs) were isolated from the patients with chronic hepatitis B by the routine method, and induced into specific DCs with HBsAg pulsed. The (3)H-TdR incorporation method was used to determine the stimulation effect of HBsAg-pulsed DCs on the proliferation of CIK cells.

[Cytoxic T lymphocyte responses to hepatitis B virus small surface antigen in BALB/c mice].

Objective: To observe cytotoxic T lymphocytes (CTL) responses induced by immunization of mice with hepatis B small surface antigen (S-HBsAg).

Methods: BALB/c(H-2d) mice were injected intraperitoneally with S-HBsAg at the doses ranging from 0-5 microg with a booster injection 2 weeks later. Four weeks after the booster immunization, the specific cytotoxic reactivity of the spleen lymphocytes isolated from the immunized mice, following stimulation by S-HBsAg specific CTL epitope peptide in vitro, with 51Cr-labeled P815 cells treated with the peptide was observed.

[Retrospective analysis on clinical features of 35 patients with severe acute respiratory syndrome].

Objective: To analyze the clinical features of severe acute respiratory syndrome (SARS).

Methods: The clinical features of 35 patients with SARS in the past five months were retrospectively studied, and compared with 35 patients with community-acquired pneumonia. Consecutive blood samples from 13 patients with SARS and 10 healthy volunteers were collected.