Persistent ER stress causes GPI anchor deficit to convert a GPI-anchored prion protein into pro-PrP via the ATF6-miR449c-5p-PIGV axis.

Endoplasmic reticulum (ER) stress and unfolded protein response are cells' survival strategies to thwart disruption of proteostasis. Tumor cells are continuously being challenged by ER stress. The prion protein, PrP, normally a glycosylphosphatidylinositol (GPI)-anchored protein exists as a pro-PrP retaining its GPI-peptide signal sequence in human pancreatic ductal cell adenocarcinoma (PDAC).

A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis.

• Seven monoclonal antibodies (mAbs) against SARS-CoV-2 nucleocapsid protein are produced, which can be applied in ELISA, WB, and IF assays. • A pair of mAbs, 2G11/bio-1C7, can detect SARS-CoV-2 nucleocapsid protein as low as 15 pg/well in the double sandwich ELISA. • The mAb, 2G11, shows 97.