[Effects of Simvastatin on Expression of Multidrug Resistance Gene and Protein of SHI-1 Cells].

Objective: To investigate the effects of simvastatin(SIM) and serum free medium(SFM) on the expression of multidrug resistance gene(MDR1) and protein of SHI-1 cells.

Methods: Trypan blue exclusion assay was used to detect the proliferation level and viability of SHI-1 cells after treatment with SIM and culture in SFM; The multi-drug resistant protein p-gp was measured by flow cytometry after culture in SFM for 1 to 3 days and treatment with various concentration of simvastatin. The effect of SFM culture and SIM treatment on the expression of MDR1 trascript was detected by qPCR; ELISA was used to measure the change of cellular cholesterol after culture in SIM and SFM.

[Comparative investigation of molecules releasing from intra-hollow calcium-alginate capsules using fluorimetry].

In this paper, the authors employ three different types of dye molecules, Nile red, Rhodamine 6G, fluorescein and a fluorescent protein-R-phycoerythrin (R-PE). The Rhodamine 6G is positively charged molecules, fluorescein is negatively charged molecules, and Nile red is neutral molecules. The R-phycoerythrins have either a net positive or negative charge which is balanced at the isoelectric point (4.