Identification of aberrantly methylated differentially expressed genes and associated pathways in endometrial cancer using integrated bioinformatic analysis.

Endometrial cancer (EC) is a fatal female reproductive tumor. Bioinformatic tools are increasingly developed to screen out molecular targets related to EC. In this study, GSE17025 and GSE40032 were obtained from Gene Expression Omnibus (GEO).

[A comparative study of phenoloxidase activity between and ].

Objective: To study the differences of the phenoloxidase (PO) relative activity among ribbed shelled , smooth shelled and .

Methods: The crude PO fluid was extracted from the soft tissue of and by homogenation and centrifugation. The PO activity was detected with catechol as the substrate in the reaction systems.

Eleven genes associated with progression and prognosis of endometrial cancer (EC) identified by comprehensive bioinformatics analysis.

Background: Endometrial cancer (EC) is one of the female malignant tumors. Endometrial cancer predominately affects post-menopausal women. Bioinformatics analysis has been widely applied to screen and analyze genes in linkage to various types of cancer progression.

identified by comprehensive bioinformatic analysis as a hub candidate biological target in human epithelial ovarian cancer.

Background: Epithelial ovarian cancer (EOC) is a female malignant tumor. Bioinformatics has been widely utilized to analyze genes related to cancer progression. Targeted therapy for specific biological factors has become more valuable.

[Preliminary study on molecular detection of polysaccharide from and its intervention to hepatic fibrosis in rats infected with ].

Objective: To detect the molecular characterization of polysaccharide purified from , so as to investigate its role of intervention to the formation of hepatic fibrosis caused by infection.

Methods: The crude polysaccharide from was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively. A total of 50 female BALB/c mice were randomly divided into five groups：A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group).

Tumor-suppressor role of miR-139-5p in endometrial cancer.

Background: Endometrial cancer (EC) is the fourth most common malignancy of the female genital tract worldwide. MicroRNAs are important gene regulators with critical roles in diverse biological processes, including tumorigenesis. Several study's show that miR-139-5p is involved in the tumorigenesis and metastasis of various cancers.

[Optimization and comparison of extraction methods of mitochondrial DNA of ].

Objective: To optimize the extraction methods of mitochondrial genome DNA (mtDNA) of .

Methods: The pyrolysis, protein K variable-temperature digestion and high-concentration potassium acetate purification were applied to optimize the high-concentration-salt precipitation method, and then the optimized method was compared with two common extraction methods, the sucrose density gradient centrifugation method and traditional high-concentration-salt precipitation method. The mtDNA samples were identified by using spectrophotometry, agarose gel electrophoresis and the amplification products of COX1.

[Composition and diversity of Acaroid mites' communities in indoor environments of kindergartens in Wuhu City].

Objective: To understand the breeding situation of Acaroid mites in indoor environments of kindergartens in Wuhu City, so as to provide the evidence for its prevention and control.

Methods: From March to June and September to December in 2014, dust samples were collected from 15 kindergartens of 3 ranks every month. Acaroid mites in the samples were isolated, identified and counted.

[Experimental study on determination of viability of Schistosoma japonicum cercariae by staining].

Objective: To determine the viability of Schistosoma japonicum cercariae by staining.

Methods: Schistosoma japonicum cercariae were stained by 0.4% trypan blue, 0.

[Seasonal changes of glucose levels in Oncomelania hupensis].

Objective: To study the seasonal changes of glucose levels per unit soft tissue of Oncomelania hupensis.

Methods: O. hupensis snails were collected from the beach of the Qingyi River in Wuhu City, Anhui Province from August 2012 to July 2013.

[Experimental study on quick determination of viability of Oncomelania hupensis by staining].

Objective: To find out a quick, simple and convenient method of determining the viability of Oncomelania hupensis.

Methods: O. hupensis snails were stained for 30 minutes by 0.

[Relationship between Yangtze River floodplain micro ecological environment and distribution of Oncomelania hupensis snails].

Objective: To explore the relationship between the Yangtze River floodplain ecological environment (vegetation, soil, water and light intensity) and the distribution of Oncomelania hupensis snails, so as to provide the evidence for ecological snail control.

Methods: Three regions (the Lu-Gang Bridge, Dragon Nest Lake in the bund, and Dragon Nest lake beach) were selected to investigate the plant characteristics (species, height, coverage, frequency and strain of clusters), soil characteristics (temperature, humidity, light intensity) and pH value. All the results were analyzed statistically with SPSS 18 software.

[Seasonal changes of glycogen level in Oncomelania hupensis].

Oncomelania hupensis snails were collected from Qingyi River of Wuhu City from August 2012 to July 2013. Livers and pedal muscles of snails were dissected. Anthrone colorimetric method was used to evaluate the glycogen concentrations of whole-body, liver and muscle.

[Extraction and morphological observation of Oncomelania hupensis haemocytes].

Haemocytes were collected from Oncomelania hupensis snails by using tissue disruption and filtration method, stained by Giemsa and methylene blue, respectively, and observed under microscope. Number of haemocytes from one snail was counted. Out of 54 haemocytes, 3 types of cells were found: big round cells with particles, small round cells with oval nuclei and spindle cells with oblong nuclei.

[Killing effect of exogenous NO on cercariae of Schistosoma japonicum in vitro].

Objective: To explore the killing effects of exogenous NO on the cercariae of Schistosoma japonicum in vitro and the blocking effects of NO inhibitors.

Methods: The cercariae of S. japonicum were collected from naturally infected snails, and then formulated into a 1000 cercariae/ml suspension with RPMI 1640 medium.

[Lysozyme activities of Oncomelania hupensis in different seasons and ages of snails].

Objective: To explore the relationship between the lysozyme activities of Oncomelania hupensis snails among different seasons, and the difference of the lysozyme activities among the different ages of the snails.

Methods: The homogenate soft tissues of O. hupensis were dissolved with Tris-HCl-TritonX-114 buffer and concentrated, and the enzyme was extracted by centrifugation.

[Isolation and purification of Oncomelania hupensis agglutinin and determination of its molecular weight].

Objective: To isolate and purify agglutinin from Oncomelania hupensis snail and determine its molecular weight.

Methods: Agglutinin was preliminarily isolated from snail tissue homogenate by 0%-40% saturated ammonium sulfate, and then successively purified with Sephadex G-75 gel filtration and Sepharose 4B affinity chromatography. Bradford assay was used to determine the protein content.

[Extraction of agglutinin from Oncomelania hupensis and its haemagglutination activity].

Objective: To explore the extraction methods of agglutinin from Oncomelania hupensis snail and study its haemagglutination activity.

Methods: Protein obtained by ammonium sulfate fractionation precipitation with 20%-100% saturation of ammonium sulfate. Its haemagglutination activity was determined by rabbit erythrocytes.

Stacking ionizable analytes in a sample matrix with high salt by a transient moving chemical reaction boundary method in capillary zone electrophoresis.

The paper presents a novel on-line transient moving chemical reaction boundary method (tMCRBM) for simply but efficiently stacking ionizable analytes in high-salt matrix in capillary zone electrophoresis (CZE). The powerful function and stability of the tMCRBM are elucidated with the ionizable test analytes of L-phenylalanine (Phe) and L-tryptophan (Trp) in the matrix with 85.6-165.

Improving separation efficiency of capillary zone electrophoresis of tryptophan and phenylalanine with the transient moving chemical reaction boundary method.

A simple and convenient mode--moving chemical reaction boundary method-capillary zone electrophoresis (MCRBM-CZE)--was designed for the enhancement of separating efficiency of CZE. In this mode, the transient MCRBM is used for the on-line pre-treatment of sample. By analyses of tryptophan (Trp) and phenylalanine (Phe) as an example, the experiments by MCRBM-CZE were carried out and further compared with those by normal CZE without the transient MCRBM.

Investigations on factors that influence the moving neutralization reaction boundary method for capillary electrophoresis and isoelectric focusing.

We investigated several factors, such as temperature, current intensity (i), time (t) and the product (mA min mm(-2), viz., C mm(-2)) of i and t, etc., that obviously affect the moving neutralization reaction boundary method (MNRBM).