[Expression of HIF-1α in the prostatic tissue of mice with high-fat diet-induced obesity].

Objective: To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) in the prostatic tissue of mice with high-fat diet-induced obesity and to explore the effect of hypoxia on BPH in obese male mice.

Methods: We randomly divided 20 C57BL/6J male mice into two groups of equal number and fed them on a high-fat diet (HFD group) or a normal diet (control group) for 17 weeks. Then, we measured the body weight, prostate weight, prostate volume and levels of serum triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and free fatty acids (FFA) by ELISA, observed the morphology of the prostate by HE staining, calculated the area of the prostatic glandular cavity, and determined the expression of HIF-1α in the prostatic tissue by immunohistochemistry.