[In vitro study of antimicrobial efficacy of different irrigations on Enterococcus faecalis biofilm formation in root canal].

Purpose: To compare the antimicrobial effect of different irrigations on Enterococcus faecalis biofilms in extracted teeth and evaluate the antimicrobial activity of irrigating solutions residual against E. faecalis biofilms formation, in order to provide a better strategy for clinician.

Methods: Extracted human premolar teeth with single root canal were clearly autoclaved.

[Effect of different stress conditions on growth and biofilm formation capability of Enterococcus faecalis].

Objective: To study the changes of growth and biofilm formation capability of Enterococcus faecalis (Ef) in different stress conditions.

Methods: The changes of growth of Ef in stress conditions were observed by measuring the A600 value with ultraviolet spectrophotometer. Ef was incubated on glass slide in stress conditions, biofilm formation capability of cells was investigated by colony-forming unit (CFU) counting of the culturable bacteria and fluorescence confocal laser scanning microscopy.