[Impact of comprehensive health education on oral care knowledge, attitude and practice in the elderly in long-term care institutions].

Purpose: To assess the impact of comprehensive health education on oral care knowledge, attitude and practice(KAP) of the elderly in the long-term care institutions and to provide references for oral health education among the elder people.

Methods: Elder people from 2 nursing centers in Wuhan were selected. The follow-up period was 6 months.

[Long-term results of dual anti-collagen membrane in guided tissue regeneration].

Objective: To evaluate the long-term clinical effect of dual anti-collagen membranes in guided tissue regeneration (GTR).

Methods: This randomized clinical trial included 26 teeth in 24 patients, presenting a total of 31 lesions consisting of intrabony defects and furcation defects. Twenty-six teeth were divided into two groups and treated by GTR with dual anti-collagen membranes and atelocollagen membranes, respectively.

[Establishment and application of an intact natural model of human dental plaque biofilm].

Purpose: Human dental plaque is implicated in a number of oral diseases. Collection of its undisturbed intact structure facilitates observing the formation and the effect of treatment. This study established a model in vivo to collect intact natural dental plaque biofilm, and observed the features and evaluated the immediate penetration and bactericidal effect of an essential oil (EO) mouthrinse on it at different periods.

[Construction and identification of recombinant human platelet-derived growth factor-B adenoviral vector and transfection into periodontal ligament stem cells].

Objective: To construct a recombinant human platelet-derived growth factor-B (PDGF-B) adenoviral vector and to transfect it into human periodontal ligament stem cells (PDLSC).

Methods: The recombinant plasmid pAd-PDGF-B was constructed by homologous recombination and confirmed by restriction endonucleases digestion. Recombinant adenovirus was packaged in HEK293 cells.

Rose bengal staining in detection of oral precancerous and malignant lesions with colorimetric evaluation: a pilot study.

Early detection of oral precancerous and malignant lesions is still a diagnostic challenge for most of clinicians, and ideal adjuncts for detection of these lesions are currently unavailable. Our preliminary study has indicated that rose bengal (RB) staining might have the potency as a diagnostic aid; however, its clinical significance and reliability in hospital-based population are still not clear. In the present study, we investigated the efficacy of RB staining in detection of oral precancerous and malignant lesions.