Publications by authors named "Shu Kuang Lee"
Background: Data on clinical outcomes of infection with variants of oncogenic human papillomavirus (HPV) types other than HPV16 and HPV18 are rare. We investigated intratypic variations in non-HPV16/18 oncogenic types and their corresponding relationships with cervical intraepithelial neoplasia grades 2-3 (CIN2/3).
Methods: Study subjects were women who were positive for one or more of 11 non-HPV16/18 oncogenic types.
Background: Little is known about the rates and determinants of oral human papillomavirus (HPV) infection, an infection that is etiologically linked with oropharyngeal cancers.
Methods: A cohort of male university students (18-24 years) was examined every 4 months (212 men, 704 visits). Oral specimens were collected via gargle/rinse and swabbing of the oropharynx.
Background: The epidemiology of high-risk (hr) human papillomavirus (HPV) infections in mid-adult women with new sex partners is undefined.
Methods: We analyzed baseline data from 518 25- to 65-year-old women online daters. Women were mailed questionnaires and kits for self-collecting vaginal specimens for polymerase chain reaction-based hrHPV testing.
Variants of human papillomavirus (HPV) type 31 have been shown to be related both to risk of cervical lesions and racial composition of a population. It is largely undetermined whether variants differ in their likelihood of persistence. Study subjects were women who participated in the ASCUS-LSIL Triage Study and who had a newly detected HPV31 infection during a two-year follow-up with six-month intervals.
View Article and Find Full Text PDFAlthough the lineages of human papillomavirus type 31 (HPV31) variants are recognized, their clinical relevance is unknown. The purpose of our study was to examine risk of cervical intraepithelial neoplasia Grades 2-3 (CIN2/3) by HPV31 variants. Study subjects were women who participated in the atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion Triage Study and who had HPV31 infections detected at one or more visits.
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- Male circumcision does not significantly affect the overall acquisition rates of clinically relevant HPV types among young men aged 18-20.
- Uncircumcised men are more likely to have the same HPV type detected in multiple sites (shaft, scrotum, urine) compared to circumcised men, indicating a higher potential for transmission.
- The findings suggest that while circumcision might not lower the risk of acquiring HPV, it could influence the extent of infection across different genital sites.
Determining the rate at which men develop genital warts after infection with alpha genus human papillomavirus (HPV) types will provide important information for the design of prevention strategies. We conducted a cohort study of 18-21-year-old men who underwent triannual genital examinations. The 24-month cumulative genital wart incidence was 57.
View Article and Find Full Text PDFHuman papillomavirus (HPV) RNA levels may be a more sensitive early indicator of predisposition to carcinogenesis than DNA levels. We evaluated whether levels of HPV-16 and HPV-18 DNA and messenger RNA (mRNA) in newly detected infections are associated with cervical lesion development. Female university students were recruited from 1990 to 2004.
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- Developed a liquid bead microarray (LBMA) assay for identifying genital human papillomaviruses (HPVs), capable of detecting as few as 50 copies per reaction.
- Compared LBMA with reverse line blot (RLB) and Hybrid Capture II (hc2) assays in two studies, showing high type-specific agreement and sensitivity for detecting HPV infections and cervical-intraepithelial neoplasia (CIN2).
- Results indicate LBMA's promising role in clinical and epidemiological research, particularly due to its high reproducibility and effectiveness.
Background: Little is known about the epidemiology of human papillomavirus (HPV) infections that persist for more than a few years.
Methods: Four to 12 years after participation in a longitudinal study of incident HPV infection, a cohort of former university students returned for a follow-up visit that included HPV genotyping of cervical and vulvovaginal swab specimens and collection of colposcopy-directed biopsy specimens.
Results: Of 147 women with HPV infection detected during their undergraduate years, 24 (16.
Background: In contrast to the wealth of data on human papillomavirus (HPV) infections in women, much less is known about HPV in men.
Methods: Between June 2003 and March 2006, a total of 240 heterosexually active male university students 18-20 years of age were recruited for participation in a cohort study of HPV infection. Genital cell samples were collected, at 4-month intervals, for HPV-DNA analysis by polymerase chain reaction.
The authors assessed young men's knowledge and perceptions of genital human papillomavirus (HPV) infection to identify factors that predict intention to make positive behavioral changes. Male university students aged 18 to 25 years completed a self-report instrument to assess knowledge and perceptions of genital HPV infection. If diagnosed with HPV, most men (95%) reported that they would use condoms with new partners.
View Article and Find Full Text PDFBackground: To determine the potential value of human papillomavirus (HPV) vaccines, information concerning the incidence and duration of clinically important lesions is needed.
Methods: A total of 603 female university students were followed for a mean duration of 38.8 months.
To evaluate methods for detection of genital human papillomavirus (HPV) DNA in men, samples were obtained from 3 consecutive groups of 10 men attending a sexually transmitted disease clinic by use of (1) a saline-wetted Dacron swab alone, (2) a saline-wetted cytobrush, or (3) emery paper (600A-grit Wetordry Tri-M-ite; 3M) abrasion followed by a saline-wetted Dacron swab. By use of a polymerase chain reaction-based assay, 45% of emery-paper samples were found to be positive for beta-globin, compared with 23% of swab-alone and 0% of cytobrush samples. Subsequently, emery paper and saline-wetted Dacron swabs were used to obtain penile shaft, glans, foreskin, and scrotum samples from 318 male university students.
View Article and Find Full Text PDFPrevious studies have characterized the prevalence and duration of serum IgG antibodies to human papillomavirus type 16 (HPV 16) in a well-studied cohort of college women, using viruslike particle- (VLP) based ELISAs. In this study IgA antibodies in cervical secretions and sera were examined using a newly developed capsomer-based ELISA and the patterns observed for serum IgG, serum IgA, and cervical IgA antibodies were compared. The median time to antibody detection from the first detection of HPV 16 DNA was 10.
View Article and Find Full Text PDFObjective: The purpose of this study was to identify clinical signs and symptoms associated with detection of human papillomavirus (HPV) DNA in the female genital tract.
Study Design: A total of 516 university students (18 to 24 years old) enrolled in a cohort study that included the collection of genital specimens for HPV DNA testing every 4 months for up to 4 years. Reported symptoms and objective clinical findings of women with and without HPV DNA were compared by multivariate analysis.
Article Synopsis
- The study tracked 603 female university students in Washington State from 1990 to 2000 to investigate HPV infection rates and transmission risks.
- At 24 months, the study found a 32.3% cumulative incidence of first-time HPV infections among participants, comparable for both virgins and nonvirgins.
- Key risk factors identified included smoking, oral contraceptive use, and having a new male partner, particularly one with a history of multiple partners; interestingly, consistent condom use did not protect against infection.
Article Synopsis
- The study explored whether using water-soluble gel lubricant on plastic vaginal specula would reduce discomfort and improve cervical cytology diagnosis rates.
- Five family planning clinics were tested, comparing gel lubricant use against water only during examinations, with pathologists unaware of which lubricant was used.
- Results showed no significant difference in unsatisfactory smear rates or overall cervical cytology outcomes between the clinics that used gel and those that did not, concluding that the lubricant does not impact cervical cytology results in young women.
Article Synopsis
- The study followed female university students who were initially free of HPV16 to track infection rates over five years.
- Among the 528 women, 62 acquired HPV16, with a higher cumulative incidence for prototype-like variants compared to non-prototype-like (NPL) variants.
- Factors such as race and recent hormonal contraceptive use were associated with a higher likelihood of detecting NPL variants, which correlated with increased risk for cervical intraepithelial neoplasia, suggesting that other biological mechanisms might influence this risk.