[Nature of factors stimulating cobalamin production by Achromobacter cobalamini].

The object of this work was to study the nature of factors contained in molasses and maize extract and stimulating cobalaminogenesis in Achromobacter cobalamini. The activity of substrate fractions was analyzed to show that the stimulating substance was precipitated on the cation exchanger and eluted from it with HCl. The factor was found to be an organic nitrogen base readily soluble in water and ethanol but insoluble in ether, chloroform and methanol.

[Effect of organic substances on the growth and cobalamin genesis of Achromobacter cobalamini].

Corn steep and molasses were shown to stimulate cobalaminogenesis in Achromobacter cobalamini. Their concentrations optimal for the culture growth and vitamin B12 synthesis were found. Corn steep at a concentration of 2% accelerated the growth.