Establishment of human iPSC line from patient of Indian ethnicity carrying homozygous CD8/9 (+G) beta thalassemia mutation.

This study shows generation of iPSCs from peripheral blood mononuclear cells (PBMNCs) of a male patient having homozygous CD 8/9 (+G) beta thalassemia (major) mutation. Cells were nucleofected with episomal vectors containing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (dominant negative p53 mutation). Cell line exhibited presence of pluripotency markers by immunofluorescence, flow-cytometry and PCR.

Development and characterization of human iPSC line NCCSi004-A from umbilical cord blood (UCB) derived CD34cells obtained from donor belonging to Indian ethnic population.

Here we report the reprogramming of CD34 cells obtained from UCB of a healthy donor female child belonging to the Indian ethnic population. These CD34cells were subjected to nucleofection for delivery of episomal vectors expressing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (negative mutation in p53). The iPSC colonies expressed pluripotency markers as detected by PCR, immunofluorescence and flow-cytometry.

Establishment of human iPSC line NCCSi003-A from CD34cells of peripheral blood collected during apheresis of healthy donor from Indian ethnicity.

We present generation of iPSCs from CD34 cells isolated from peripheral blood, collected during apheresis of a healthy female individual. We nucleofected the CD34cells by episomal vectors containing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (dominant negative mutation in p53). The resultant colonies showed cobble-stone appearance and stained positive for alkaline phosphatase.