In vitro cytotoxic and in silico activity of piperine isolated from Piper nigrum fruits Linn.

Background: Piper nigrum [Piperaceae], commonly known as black pepper is used as medicine fairly throughout the greater part of India and as a spice globally.

Purpose: To isolate piperine and evaluate in vitro cytotoxic [antiproliferative] activity and in silico method.

Methods: Piperine was isolated from the fruits of P.

Phytochemical analysis and hepatoprotective properties of Tinospora cordifolia against carbon tetrachloride-induced hepatic damage in rats.

The present study was conducted to evaluate the hepatoprotective activity of different extracts of Tinospora cordifolia against carbon tetrachloride (CCl4) induced liver damage in rats. The pet ether, ethanol and aqueous extracts of various parts of the plant such as leaf, stem and root were tested at the dose of 200mg/kg body weight orally using Wistar albino rats and Silymarin was given as reference standard. Ethanolic extract of all the parts showed significant hepatoprotective effect by reduction in serum enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin (TBL) in the selected model which is followed by aqueous and pet ether extracts.