Selenium containing tetraphenyl substituted BODIPY probe was successfully synthesized from respective selenium aldehyde and tetraphenyl pyrrole using Knoevenagel-type condensation. The product was characterized using various spectroscopic techniques ( H, C, Se, B, and F) and mass spectrometry. The probe was found to be selective and sensitive towards detection of superoxide over other ROS with a "turn-off" (quenched) fluorescence response.
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