Publications by authors named "Shouta Araki"
Article Synopsis
- Interleukin-11 (IL-11) is a cytokine important for various biological processes, including bone metabolism, and can enhance the expression of bone sialoprotein (BSP) in osteoblast-like cells.
- When IL-11 is introduced, it activates luciferase activity linked to specific regulatory elements in the BSP gene promoter, indicating its role in gene transcription.
- The stimulation of BSP transcription by IL-11 involves several transcription factors and is regulated through specific binding sites, with inhibition studies suggesting the involvement of multiple signaling pathways.
Article Synopsis
- - Bone sialoprotein (BSP) plays a key role in mineralized tissues and is involved in the formation of hydroxyapatite, while forskolin (FSK) and fibroblast growth factor 2 (FGF2) are known to enhance the proliferation and differentiation of osteoblasts.
- - In prostate cancer DU145 cells, FSK and FGF2 significantly increased the mRNA and protein levels of BSP and Runx2, demonstrating their potential to influence bone-related processes in cancer cells.
- - Experiments showed that the mechanisms by which FSK and FGF2 enhance BSP transcription involve specific regulatory elements (CRE1 and CRE2) in the BSP gene promoter, and that these processes can be
Article Synopsis
- - Bone sialoprotein (BSP) is an important glycoprotein involved in bone tissue, while fibroblast growth factor 2 (FGF2) acts as a strong growth factor for various cells, including osteoblasts.
- - Previous research showed that FGF2 influences the transcription of the BSP gene by interacting with specific binding sites in the gene's promoter region.
- - This study found that FGF2 increased the levels of BSP and Runx2 mRNA in MCF7 breast cancer cells and enhanced luciferase activity in BSP gene promoter constructs, demonstrating that FGF2 stimulates BSP transcription by targeting certain DNA elements.
Article Synopsis
- Inorganic polyphosphate (poly(P)) is found in nearly all cells and plays important biological roles, especially in osteoblasts related to bone function.
- The study showed that sodium phosphate glass type 25 (SPG25) increased bone sialoprotein (BSP) mRNA levels in osteoblast-like cells and impacted luciferase activities linked to specific gene constructs.
- The effects of SPG25 on BSP transcription were shown to be mediated by key binding sites (FGF2 response element and HOX) and could be inhibited by various kinase inhibitors, indicating complex signaling pathways in bone mineralization.
Article Synopsis
- PDGF is produced by mesenchymal cells and platelets, stimulating the growth of osteoblasts and increasing the activity of osteoclasts, which leads to bone resorption.
- In experiments with Saos2 and ROS17/2.8 osteoblast-like cells, PDGF-BB significantly increased the levels of bone sialoprotein (BSP) mRNA and protein over time.
- The study found that PDGF-BB activates specific elements in the BSP gene promoter, suggesting that it regulates BSP transcription through pathways involving cAMP response elements and AP1, with potential involvement from various protein kinases.
Parathyroid hormone (PTH) regulates serum calcium and inorganic phosphate levels through its actions on kidney and bone. Bone sialoprotein (BSP) is an early marker of osteoblast differentiation and bone metabolism. We here report that two cAMP response elements (CRE) in the human BSP gene promoter are target of PTH.
View Article and Find Full Text PDFBone sialoprotein (BSP) is an early marker of osteoblast differentiation. We previously reported that fibroblast growth factor 2 (FGF2) regulates BSP gene transcription via FGF2 response element (FRE) in the proximal promoter of rat BSP gene. We here report that activator protein 1 (AP1) binding site overlapping with glucocorticoid response element (GRE) AP1/GRE in the rat BSP gene promoter is another target of FGF2.
View Article and Find Full Text PDFBone sialoprotein (BSP) is an early marker of osteoblast differentiation. Androgens are steroid hormones that are essential for skeletal development. The androgen receptor (AR) is a transcription factor and a member of the steroid receptor superfamily that plays an important role in male sexual differentiation and prostate cell proliferation.
View Article and Find Full Text PDFQuercetin is a typical flavonol-type flavonoid and is present in a variety of vegetables, and their antioxidant effect implies their possible role in the prevention of oxidative stress related chronic diseases. Bone sialoprotein (BSP) is a noncollagenous protein of the extracellular matrix in the mineralized connective tissues that has been implicated in the nucleation of hydroxyapatite crystals. Previously, we reported that isoflavone (genistein) activated BSP gene transcription is mediated through an inverted CCAAT box in the proximal BSP gene promoter.
View Article and Find Full Text PDFInsulin-like growth factor-I (IGF-I) promotes bone formation by stimulating proliferation and differentiation of osteoblasts. Bone sialoprotein (BSP), is thought to function in the initial mineralization of bone, is selectively expressed by differentiated osteoblast. To determine the molecular mechanism of IGF-I regulation of osteogenesis, we analyzed the effects of IGF-I on the expression of BSP in osteoblast-like Saos2 and in rat stromal bone marrow (RBMC-D8) cells.
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