Functional analysis revealed the involvement of ZmABCB15 in resistance to rice black-streaked dwarf virus infection.

Background: Maize rough dwarf disease (MRDD), caused by rice black-streaked dwarf virus (RBSDV) belonging to the Fijivirus genus, seriously threatens maize production worldwide. Three susceptible varieties (Ye478, Zheng 58, and Zhengdan 958) and two resistant varieties (P138 and Chang7-2) were used in our study.

Results: A set of ATP-binding cassette subfamily B (ABCB) transporter genes were screened to evaluate their possible involvements in RBSDV resistance.

Genome wide association analysis for yield related traits in maize.

Background: Understanding the genetic basis of yield related traits contributes to the improvement of grain yield in maize.

Results: Using 291 excellent maize inbred lines as materials, six yield related traits of maize, including grain yield per plant (GYP), grain length (GL), grain width (GW), kernel number per row (KNR), 100 kernel weight (HKW) and tassel branch number (TBN) were investigated in Jinan, in 2017, 2018 and 2019. The average values of three environments were taken as the phenotypic data of yield related traits, and they were statistically analyzed.

Multi-Omics Analyses Reveal the Regulatory Network and the Function of ZmUGTs in Maize Defense Response.

Maize is one of the major crops in the world; however, diseases caused by various pathogens seriously affect its yield and quality. The maize mutant (mt) caused by the intragenic recombination between two nucleotide-binding, leucine-rich repeat (NLR) proteins, exhibits autoactive hypersensitive response (HR). In this study, we integrated transcriptomic and metabolomic analyses to identify differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) in mt compared to the wild type (WT).

Genomic selection on shelling percentage and other traits for maize.

Genomic selection (GS) is the one of the new method for molecular marker-assisted selection (MAS) that can improve selection efficiency and thereby accelerate selective breeding progress. In the present study, we used the exotic germplasm LK1 to improve the shelling percentage of Qi319 by GS. Genome-wide marker effects for each trait were estimated based on the performance of the testcross and SNP data for F progenies in the training population.

Exogenous Pi supplementation improved the salt tolerance of maize (Zea mays L.) by promoting Na exclusion.

The mechanism of phosphate (Pi)-mediated salt tolerance in maize is poorly understood. In this study, the effects of Pi (HPO) on the salt tolerance of two contrasting genotypes was investigated in a pot experiment. We discovered that the application of 3 mM Pi could alleviate salt injury caused by 200 mM NaCl.

[Survey of study on the extraction, purification and determination methods of glycyrrhizic acid in Licorice].

Extraction, purification and determination methods of glycyrrhizic acid in Licorice are surveyed in this paper. The extracting efficiency of dilute ethanol solvent for glycyrrhizic acid is higher than water extraction, and ammonia appended to ethanol solution can increase the effect of extraction. Ultrasound method, microwave-assisted method and supercritical CO2 fluid extraction are more effective than the conventional techniques, due to the short extraction time, low consumption of solution and energy.