Development of a genetic transfer system in selenate-respiring bacterium Citrobacter sp. strain JSA which was isolated from natural freshwater sediment.

Conjugative mating between the selenate-reducing bacterium Citrobacter sp. strain JSA and Escherichia coli S17-1 harboring the broad-host-range plasmid pKT230 or pKT240 (IncQ) allowed genetic transfer to strain JSA at a maximum frequency of 2.5×10(-5) (pKT230) and 5.