The expression of cytokines in aqueous humor of high myopic patients with cataracts.

Purpose: To analyze the expression of 440 human cytokines in aqueous humor of high myopic patients with cataracts.

Methods: Eighty-five patients with cataracts were recruited in this study. In the screening stage, the RayBio G-Series Human Cytokine Antibody Array 440 was used to assay the aqueous humor samples collected from nine high myopic patients with cataracts and eight non-myopic patients with cataracts right before the surgery.

Effect of Aβ protein on inhibiting proliferation and promoting apoptosis of retinal pigment epithelial cells.

Aim: To identify the effect and regulatory mechanism of amyloid β (Aβ) protein on retinal pigment epithelial (RPE) cells in cell proliferation and apoptosis, and clarify Aβ role in the pathogenesis of age-related macular degeneration (AMD).

Methods: The model of Aβ25-35 protein cytotoxicity in RPE cell was successfully established to investigate the effect of Aβ protein on RPE cells . Based on Aβ protein, the specific inhibitors (HY-50682 or BAY11-7082) or activating agent (lipopolysaccharide) was used to analyze the regulatory mechanism of Aβ protein to RPE cells on cell proliferation and apoptosis by flow cytometry, real-time polymerase chain reaction, Western blotting, enzyme-linked immunosorbent assay and dual-luciferase reporter gene assay.

Nrf2 protects human lens epithelial cells against HO-induced oxidative and ER stress: The ATF4 may be involved.

Our previous study has shown heme oxygenase-1 (HO-1) protects human lens epithelial cells (LECs) against HO-induced oxidative stress and apoptosis. Nrf2, the major regulator of HO-1, is triggered during the mutual induction of oxidative stress and ER stress. In response to ER stress, unfolded protein response (UPR) serves as a program of transcriptional and translational regulation mechanism with PERK involved.

miRNA-1273g-3p Involvement in Development of Diabetic Retinopathy by Modulating the Autophagy-Lysosome Pathway.

BACKGROUND Diabetic retinopathy (DR) is one of the most common and serious complications of diabetes mellitus (DM). The autophagy-lysosome pathway (ALP) is one of the main intracellular self-digestive degradation systems. Lysosomal impairment and autophagic dysfunction are early events in the pathogenesis of DR, suggesting autophagy might be a novel therapeutic strategy for DR treatment.

Efficacy comparison between manual small incision cataract surgery and phacoemulsification in cataract patients: a meta-analysis.

Objective: Systematic review of manual small incision cataract surgery (MSICS) and phacoemulsification (PHACO) on the postoperative visual quality and surgical complications.

Methods: Relevant literatures on clinical efficacy of PHACO and MSICS were included by retrieving in Medline, PubMed, Chinese Biomedical Literature Database (CBM) and Chinese Academic Journal (CNKI) databases. Meta-analysis was conducted by RevMan5.

[Effects of matrix metalloproteinases inhibitor GM6001 on choroidal neovascularization].

Objective: To explore the efficiency of GM6001 for the inhibition of choroidal neovascularization.

Methods: Experimental study. Twenty-four Brown Norvy (BN) rats after photocoagulation were randomly divided into 3 groups as GM6001 group, DMSO group and CONT group.

IL-33 is induced by amyloid-β stimulation and regulates inflammatory cytokine production in retinal pigment epithelium cells.

Age-related macular degeneration (AMD) is the predominant cause of irreversible blindness in the elderly population. Despite intensive basic and clinical research, its pathogenesis remains unclear. However, evidence suggests that immunological and inflammatory factors contribute to the pathogenesis of AMD.

[Alternative splicing of vascular endothelial growth factor A and ocular neovascularization].

Ocular neovascularization is the primary cause of blindness in a wide range of ocular diseases. The vascular endothelial growth factor A (VEGF-A) is the key factor involved in ocular angiogenesis, which can cause eye diseases through the development of pathological angiogenesis and increase of vascular permeability. There are two families of VEGF-A isoforms formed by alternative splicing, the angiogenic VEGF-A family (VEGF(xxx)), known to contribute to ocular neovascularization, and the anti-angiogenic VEGF-A family (VEGF(xxx)b), which is found in normal ocular tissues but downregulated in human diabetic retinopathy.

[Protective effects of puerarin on lens epithelial cells in rat diabetic cataract].

Objective: The therapeutic effects of general and local applications of puerarin in the treatment of streptozotocin (STZ)-induced rat diabetic model were compared.

Methods: Experimental research. We equally divided normal Sprague-Dawley (SD) rats into a STZ group, a peritoneal injection group, a peribulbar injection group and a control group.

[Some attentive issues in the selection of intraocular lenses].

Different kinds of new intraocular lenses (IOL) provide more choices for different clinical demands. However, there are many misunderstandings about how to select the proper IOL. These misunderstandings arise from the believes that include "the expensive IOL is good IOL", "new product is absolutely perfect", etc.

Peroxynitrite-induced expression of inducible nitric oxide synthase and activated apoptosis via nuclear factor-kappa B pathway in retinal pigment epithelial cells and antagonism of cholecystokinin octapeptide-8 in vitro.

Aim: To explore that if peroxynitrite induced the expression of inducible nitric oxide synthase (iNOS)via nuclear factor-kappa B (NF-κB) pathway in retinal pigment epithelial (RPE) cells and the antagonism of cholecystokinin octapeptide-8 (Melatonin, CCK-8) in vitro.

Methods: RPE cells were obtained from eyes of C57BL/6 mouse and divided into control, peroxynitrite and CCK-8 groups. Control group was treated with saline, peroxynitrite group was treated with peroxynitrite, and CCK-8 group was treated with CCK-8 after added with peroxynitrite.

Toxicity of endogenous peroxynitrite and effects of puerarin on transplanted retinal pigment epithelial sheets in the subretinal space in mice.

Aim: To evaluate the toxicity of endogeneous peroxynitrite on transplanted retinal pigment epithelial (RPE) sheets and the effect of puerarin on their survival in the C57BL/6 mice after RPE sheets have been transplanted into SD rats' subretinal space .

Methods: C57BL/6 mice eyes were used to culture RPE cells. Ninety-six SD rats were involved in the experiment.

Effect of puerarin on retinal pigment epithelial cells apoptosis induced partly by peroxynitrite via Fas/FasL pathway.

Aim: To evaluate the peroxynitrite (ONOO(-)) of puerarin on retinal pigment epithelial (RPE) cells apoptosis induced partly by peroxynitrite via Fas/FasL.

Methods: RPE cells from C57BL/6 mice eyes were cultured. Diabetes was induced in Sprague-Dawley (SD) rats by streptozotocin (STZ) intraperitoneal injection.

[Several notable problems in current development of cataract surgery].

The technologies about cataract surgery and intraocular lens has been developing expeditiously. However, some problems have been occurring and should be corrected. Cataract blindness can not restore the sight after surgery has been performed still existed.

[MicroRNA and progresses in the region of ophthalmology].

MicroRNAs (miRNAs) consist of a growing class of short non-coding RNAs (ncRNAs) that negatively regulate the expression of genes involved in development, differentiation, proliferation, apoptosis and other important cellular processes. They regulate gene expression at the post-transcriptional level by either degradation or translational repression of a target mRNA. Nowadays more than 400 miRNAs have been identified in the human genome.

[To recognize monovision correctly and matching different types of intraocular lenses in two eyes].

The production of different kinds of novel intraocular lens (IOLs) with various functions plays an active role in improving cataract surgery by providing more choices to meet different clinical demands. However, each type of IOL has its own advantages and disadvantages. Therefore, it is important to implant two different kinds of IOLs into two eyes of a patient, which can optimize the advantages of these two IOLs and can obtain a better mixing vision.

[The preliminary study of Phosphomannopentaose sulfate (PI-88) on the experimental choroidal neovascularization].

Objective: To probe the intervention and mechanism of the inhibitor of heparanase (PI-88) on the experimental CNV model .

Methods: Experimental CNV was induced by laser photocoagulation in 29 mail (Brown Norway) BN rats (647 nm wave length Krypton laser, 360 mW power, 50 microm spot size, 0.05 second duration), and randomly divided into vacant control group, physiologic saline control group, preventive group and treated group, another 3 rats were acted as normal group, 15 days continuous intraperitoneal injection of PI-88 (25 mg kg(-1) d(-1) was administrated in preventive group (PI-88 was administered the same day as photocoagulation) and treated group (PI-88 was administered 1 week after photocoagulation when CNV had been formed), for the physiologic saline control group, PI-88 was replaced by physiologic saline.

[Screening and identification of abnormal expression proteins from hypoxia human retinal pigment epithelium cell in vitro].

Objective: To screen and identify the abnormal proteins expressed by hypoxia human retinal pigment epithelium (RPE) in vitro.

Methods: It was a experimental study. Protein of normal/hypoxia human RPE cells in exponential phase of growth was extracted, and frozen by liquid nitrogen for proteome study.

[To guard against mistakes in selection of intraocular lenses].

Development of different kinds of new intraocular lenses (IOL) provide more selections to meet various clinical requirements, which may plays an active role in making cataract surgery more perfectible. But there are many misunderstandings about how to select the proper IOL. For example, "the expensive one is the best one", "new product is absolutely perfect", and so on.

[The effect of puerarin on prevention of oxidative damage of cultured lens capsule epithelial cells].

Objective: To investigate the peroxynitrite damage to the lens epithelial cells (LEC) and the prevention of this damage by puerarin in vitro.

Methods: This paper was experimental study. Rabbit LEC were isolated and cultured and the third or forth passage LEC were used in this experiment The experiment groups included: (1) CONTROL GROUP: Heat-pathogen free saline (NS) 200 microl was added to the medium; (2) ONOO- group: ONOO- 200 microl was added to obtain the terminal concentration at 0.

[Research advances of proteome on ophthalmology].

The investigation of proteome can not only show the rules of vital movement, but also play an important role to illuminate the mechanism of genesis and development on certain diseases and that may find a new way to treat those. Using the compare analysis of proteome of physiology and pathology of individual, we can find the specific protein molecule of those diseases, provide identification markers of the diseases for early diagnosis, and point out the molecule target of the new drugs. Here we reviewed the applications of proteomic in the field of ophthalmologic research.

[Expression of liposome-mediated PEDF-GFP gene in the retina of the BN rats through different gene delivery route].

Objective: Transducing PEDF-GFP plasmid to the retina of the BN rats with cationic liposome through different gene delivery route, then observe the expression and location of the PEDF-GFP.

Methods: PEDF-GFP plasmid with cationic liposome was delivered to the retina of the BN rat through subretinal injection and intravitreal injection. The expression of GFP was observed under fluorescence microscope, and the mRNA of PEDF gene was detected by RT-PCR.

[The dynamic study of retinoic acid and its alginate sodium microspheres in rabbit eye].

Objective: To obtain a intravitreous delay release system by injection of biodegradable polymers (alginate sodium-retinoic acid microspheres, AGS-RA microspheres) to the rabbit vitreous and study the release character of RA delivery system in vitro.

Methods: RA was mixed with 1.5% alginate sodium and microspheres was made by a special electrostatic generator after dissolved in organic solvent; The content of AGS-RA in the microspheres and its release from the microspheres were measured by spectrophotometer.

Puerarin decreases lens epithelium cell apoptosis induced partly by peroxynitrite in diabetic rats.

The present study was designed to observe if puerarin decreases lens epithelium cell (LEC) apoptosis induced partly by peroxynitrite (ONOO(-)). One hundred and eight rats were randomly divided into control group (n=36), streptozotocin (STZ) group (n=36) and STZ + puerarin group (n=36). The rats in the control group intraperitoneally (i.

The antagonism of cholecystokinin octapeptide-8 to the peroxynitrite oxidation on a diabetic cataractal rat model.

Background: Cataracts is considered be formed because of an abnormal glucose metabolic pathway or oxidative stress. We explored the damaging role of ONOO- and antagonism of cholecystokinin octapeptide-8 (CCK-8) in diabetic cataractal rat lenses.

Methods: A diabetic cataractal animal model was established by peritoneal injection of streptozotocine (STZ).