Potent antitumor efficacy of human dental pulp stem cells armed with YSCH-01 oncolytic adenovirus.

Background: Systemic administration of oncolytic adenovirus for cancer therapy is still a challenge. Mesenchymal stem cells as cell carriers have gained increasing attention in drug delivery due to their excellent tumor tropism, immunosuppressive modulatory effects, and paracrine effects. However, the potential of human dental pulp stem cells (hDPSCs) loaded with oncolytic adenovirus for cancer biotherapy has not been investigated yet.

[Effect of GLUD1 on proliferation, osteogenic differentiation and mineralization of human dental pulp stem cells].

Purpose: To investigate the effect of glutamate dehydrogenase 1 (GLUD1) on proliferation, osteogenic differentiation and mineralization of human dental pulp stem cells (hDPSCs).

Methods: hDPSCs were isolated by tissue-explant method in vitro, and shGLUD1 lentivirus was transfected to knock down the expression of GLUD1. RT-PCR and Western blot were performed to detect the expression of GLUD1.

Local Inflammation Alters MMP-2 and MMP-9 Gelatinase Expression Associated with the Severity of Nifedipine-Induced Gingival Overgrowth: a Rat Model Study.

Nifedipine-induced gingival overgrowth (NIGO) is characterized by cell proliferation and extracellular matrix (ECM) component accumulation in gingival connective tissues, with varying degrees of inflammation and fibrosis. Impaired collagen and ECM homeostasis may be among the underlying molecular mechanisms that lead to the fibrotic changes that occur in drug-induced gingival overgrowth (DIGO). Because matrix metalloproteinases (MMPs) play vital roles in regulating collagen and ECM metabolism, many studies have been performed to reveal the relationship between MMPs and DIGO.

Expression of dentine sialophosphoprotein in mouse nasal cartilage.

Objective: Dentine sialophosphoprotein (DSPP) was initially thought to be unique for dentine formation during tooth development, whilst recent reports have shown a much broader expression pattern such as in bone, periodontium and inner ear. Our goal was to explore its expression and potential impact during early nasal cartilage formation in comparison with tooth development.

Study Design: We investigated DSPP expression in the nasal cartilage by immunohistochemistry and in situ hybridisation.

[Three-dimensional finite element analysis of effect of root canal taper and post on tooth stress distribution].

Objective: To evaluate the effect of root canal taper and post on tooth stress distribution.

Methods: Three-dimensional finite element models of human mandibular first molar with root canals prepared with 35# K file, ProTaper and Profile were established. The tooth were restored with fiber-resin, stainless steel and silver amalgam posts respectively.

[Maxillary second molar with five root canals: a case report].

Root canals have a lot of variations in maxillary second molar and most molar have only three root canals. The emergence of 4 root canals in maxillary second molar with two palatal root canals is especially rare. A case of 5 root canals in maxillary second molar with two palatal root canals and two mesiobuccal root canals was successfully treated and reported in this article.

[Effect of cyclic strain on cell morphology, viability and proliferation of human dental pulp cells in vitro].

Purpose: To investigate the effect of mechanical strain on cell morphology, viability and proliferation of human dental pulp cells in vitro.

Methods: Human dental pulp cells were cultured and subjected to 2% or 8% strain with Flexcell Tension Plus System for 0.5 hour, 12 hours and 24 hours, respectively, and then the cell morphology, viability and proliferation were examined by phase contrast microscope, trypan-blue staining and MTT method.

[Root canal treatment of mandibular first premolar with 3 root canals: a case].

The occurrence of three canals in mandibular premolars is very rare. This article reported and discussed the treatment of a mandibular first premolar with 3 root canals, specially in aspect of root-detecting and root-shaping.

[Survey of root canal curvature of anterior teeth from Chuang population].

Objective: To investigate the root canal curvature of permanent anterior teeth from Chuang population.

Methods: 245 anterior teeth from Chuang population were collected and examined by X-ray radiography both from labiolingual and mesiodistal directions. For 218 type I anterior teeth, degree of root canal curvature, radius of curvature and length of the curved part of root canal were measured by a special electronic vernier caliper according to Schneider's and Schäfer's method and the data obtained were analyzed.

Root canal morphology of permanent maxillary teeth in the Han nationality in Chinese Guanzhong area: a new modified root canal staining technique.

Introduction: The purpose of this study was to investigate the canal morphology of 504 maxillary permanent teeth of subjects of Han nationality in Chinese Guanzhong area.

Methods: Maxillary permanent teeth were randomly collected in Guanzhong area. After regular preparation, the teeth were immersed into ink without preparing access cavities and then put into hyperbaric oxygen chamber (0.

[Influence of soft-start irradiation on polymerization shrinkage stress of universal hybrid composite resins].

Objective: To compare the difference between soft-start curing mode and standard curing mode in polymerization shrinkage stress of universal hybrid composite resins and to study effect of the soft-start curing mode on the decrease of shrinkage stress.

Methods: Three universal hybrid resins (A: Charisma, B: TPH Spectrum, and C: Esthet-X) were respectively filled in cavities (4 mm in diameter) of epoxide resin disks, 16 specimens of each. Off them, eight of the specimens for each composite resin were exposed using soft-start mode and the other eight using standard mode.

[Effect of composite restoration on the reinforcement of teeth].

Objective: To determine the effect of composite restoration on reinforcement of weakened tooth structure and the possible mechanism.

Methods: Sixty freshly extracted non-carious maxillary premolars were collected and divided into 6 groups with 10 specimen in each group. MOD cavities (buccolingual width: 2.

[The effect of core binding factor alpha1 on transcriptional regulation of mouse dentin sialophosphoprotein].

Purpose: To investigate the effect of core binding factor alpha1 (cbfalpha1) on transcriptional regulation of mouse dentin sialophosphoprotein (DSPP) gene.

Methods: The MDPC-23 cells and the segment of nt -2475bp to +53bp were chosen. After co-transfected, the MDPC-23 cells were measured for luciferase activity using the dual luciferase reporter assay system.

[Transcriptional regulation of dentin sialophosphoprotein by c-Jun/c-Fos].

Objective: To investigate the role of c-Jun and c-Fos as transcriptional factors in regulation of dentin sialophosphoprotein (DSPP) gene by a promoter-luciferase reporter gene construct in odontoblast cell line MDPC-23.

Methods: Endogenous c-Jun or c-Fos protein was determined by immunocytochemistry. The role of c-Jun or c-Fos in transcription of DSPP was investigated in co-transfection experiments using promoter-luciferase reporter gene construct containing the sequence between -791 bp and +54 bp of mouse DSPP gene.

[Immortalized human odontoblast-like cell line expressed dentin extracellular matrix in vitro].

Objective: To determine whether dentin matrix proteins were expressed by the human odontoblast-like cell line hTERT-hOd-1 in vitro.

Methods: Collagen type I, bone sialoprotein (BSP), dentin matrix protein 1 (DMP1) and the marker for odontoblast, dentin sialophosphoprotein (DSPP) and dentin sialoprotein (DSP) were detected in these cells by immunohistochemistry, RT-PCR and in situ hybridization. During being cultured in mineralizing medium for 5 weeks, the secretion of OC and activity of ALP were measured once a week.

Smad protein mediated transforming growth factor beta1 induction of apoptosis in the MDPC-23 odontoblast-like cell line.

Objective: The function of apoptosis and its regulation in odontoblasts remain unclear. In this study, we characterize the possible role of transforming growth factor (TGF)-beta 1 in the induction of apoptosis and the molecular mechanisms that mediate TGF-beta1-induced apoptosis in odontoblasts.

Methods: Annexin V/propidium iodide staining, cell Death Detection ELISA and DNA ladder were used to examine the effect of TGF-beta1 on apoptosis in a mouse odontoblast-like cell line, MDPC-23.

[Role of Smad signaling in transcription of Smad7 gene mediated by TGF-beta1 in odontoblast cell line MDPC-23].

Purpose: To investigate the role of Smad signaling in transcription of Smad7 gene mediated by TGF-beta1 in odontoblast cell line MDPC-23, and to explore the molecular mechanism of Smad7 gene expression mediated by TGF-beta1 at the transcriptional level.

Methods: Smad function and its role in transcription of Smad7 were investigated in cotransfection experiments using Smad7 promoter-luciferase reporter construct containing the sequence between -408 bp and +112 bp of mouse Smad7 gene. The data were analysed by one-way ANOVA.

[Cloning and sequencing of the upstream of mouse dentin sialophosphoprotein promoter].

Objective: To clone and sequence the upstream of mouse dentin sialophosphoprotein promoter.

Methods: Genomic DNA was obtained from Balb/c mouse blood. The upstream of mouse dentin sialophosphoprotein promoter segments was obtained by PCR.

[Bone morphogenetic protein-2-induced alpha 2 (I) collagen expression in odontoblastic MDPC-23 cells mediated by Smad proteins].

Objective: To characterize the role of Smads proteins in alpha 2 (I) collagen (COL1A2) gene expression induced by bone morphogenetic protein-2 (BMP-2) in odontoblast cell line MDPC-23.

Methods: Endogenous Smad protein expression was determined by immunocytochemistry. Smads function and their role in COL1A2 gene expression were investigated in cotransfection experiments using promoter-luciferase reporter gene construct.

TGF-beta activated Smad signalling leads to a Smad3-mediated down-regulation of DSPP in an odontoblast cell line.

Objective: Transforming growth factor-beta (TGF-beta) regulates odontoblast differentiation and stimulates dentine extracellular matrix synthesis. However, until recently, the molecular mechanisms of action of TGF-beta have been unknown. Smad proteins have recently been identified as intracellular signalling mediators of TGF-beta.

[Effects of dental cements on the stress distribution in amalgam-restored teeth].

Objective: To evaluate the effects of 5 kinds of base materials on the stress distribution and deflection in amalgam- restored teeth.

Methods: A computerized two-dimensional finite-element of the tooth model first molar was established. Various dental cements with a thickness of 0.