[Cloning of Rcet3, a novel gene related to family 2 cystatins].

Objective: To clone the full-length Rcet3 gene, a novel gene related to family 2 cystatins, from mouse testis or other tissues.

Methods: Rcet3 gene was cloned using digital differential display (DDD) and RT-PCR was performed for cloning the full-length Rcet3 gene from adult mouse testis cDNA library with sequence analysis.

Results: Rcet3 cDNA was 610 bp in length, consisting of 4 exons to encode a protein with 140 amino acid residues.