HbMyb1, a Myb transcription factor from Hevea brasiliensis, suppresses stress induced cell death in transgenic tobacco.

Tapping panel dryness (TPD) is a complex physiological syndrome found widely in rubber tree (Hevea brasiliensis) plantations that causes severe yield loss in natural rubber-producing countries. In an earlier study, we confirmed that there is a negative correlation between HbMyb1 expression and TPD severity. To further investigate the function of HbMyb1 in TPD, HbMyb1 was over-expressed in tobacco controlled by a CaMV 35S promoter.

[Transformation of Volvariella volvacea with a thermal hysteresis protein gene by particle bombardment].

A cDNA encoding a thermal hysteresis protein was isolated from the Swedish Arctic insect spruce budworm by RT-PCR amplification. Volvariella volvacea strain V34 was transformed with this cDNA through particle bombardment. PCR detection and Southern blotting analysis show that the thermal hysteresis protein gene is integrated into Volvariella volvacea genome.

[Expression and cloning of cDNA encoding 43 kD rubber particle membrane protein of Hevea brasiliensis].

A rubber particle protein with apparent molecular mass of 43 kD as determined by SDS-PAGE was purified. A degenerate oligonucleotide primer based on the N-terminal amino acid sequence of this purified protein was used to amplify a 1385 bp cDNA by 3' rapid amplification of cDNA ends (3'RACE). The cDNA contains five repeats in a head-to-tail arrangement without intervening sequences, each encoding a ubiquitin unit of 76 amino acids.

Constitutive expression of human angiostatin in Pichia pastoris using the GAP promoter.

The GAP gene promoter was amplified from P. pastoris GS115 and used to replace the AOX1 promoter (P(AOX1)) on pPIC9K resulting in plasmid pGAP9K. The recombinant expression vector pGAP9K-AS was constructed by inserting the angiostatin gene(AS) into pGAP9K.