Clinical features and linkage analysis for a Chinese family with autosomal dominant central areolar choroidal dystrophy.

Background: A Chinese family with autosomal dominant central areolar choroidal dystrophy (CACD) was identified. The purpose of this study was to collect the clinical findings from the family and to identify the genetic entity by linkage analysis.

Methods: Forty-three individuals from 3 generations of the family underwent ophthalmologic examinations, including best-corrected visual acuity, examination of the anterior segments, and inspection of the ocular fundus after pharmacologic mydriasis.

[Molecular identification of wheat granule bound starch synthase gene polymorphism].

Fourteen wheat cultivars were identified into six types of Wx proteins combinations using 6% SDS-PAGE. PCR primers were designed according to the three Wx genes sequences and their mutants, respectively. A 327 bp-band was amplified from the Wx-A1 mutant,while the band was absent for the normal alleles at the Wx-A1 locus,as well as the presence or absence of a 187 bp PCR fragment at the Wx-B1 locus and a 700 bp PCR fragment at the Wx-D1 locus, respectively, corresponding to the normal and mutant alleles.

[Development and application of a genome specific PCR marker for Haynaldia villosa].

Random amplified polymorphic DNA (RAPD) analysis was performed on common wheat Chinese Spring, H. villosa, addition lines of H. villosa chromosome in CS, substitution line 3V of H.

[Identification, mapping, and application of a chromosome-specific RAPD marker from Haynaldia villosa].

Random amplified polymorphic DNA (RAPD) analysis was performed on common wheat of Chinese Spring, addition lines of H. villosa chromosome in CS and H. villosum from different accessions with 100 random 10-base primers.