Non-viral, high throughput genetic engineering of primary immune cells using nanostraw-mediated transfection.

Transfection of proteins, mRNA, and chimeric antigen receptor (CAR) transgenes into immune cells remains a critical bottleneck in cell manufacturing. Current methods, such as viruses and bulk electroporation, are hampered by low transfection efficiency, unintended transgene integration, and significant cell perturbation. The Nanostraw Electro-actuated Transfection (NExT) technology offers a solution by using high aspect-ratio nanostraws and localized electric fields to precisely deliver biomolecules into cells with minimal disruption.

Communicating certainty via verbal probability phrases: comparing health contexts with no context.

Introduction: Medical professionals often use verbal phrases to communicate uncertainties and certainties with their patients and the general public.

Objectives: This study aimed to investigate factors that can influence people's interpretation of probability phrases of certainty and uncertainty communicated by doctors in health and medical settings.

Methods: An experimental study with a randomized factorial design was conducted to examine both context-related factors and individual difference factors on participants' interpretation across directions of phrases and frames of the context.

Contrastive Graph Representation Learning with Adversarial Cross-View Reconstruction and Information Bottleneck.

Graph Neural Networks (GNNs) have received extensive research attention due to their powerful information aggregation capabilities. Despite the success of GNNs, most of them suffer from the popularity bias issue in a graph caused by a small number of popular categories. Additionally, real graph datasets always contain incorrect node labels, which hinders GNNs from learning effective node representations.

[The 510th case: fever of unknown origin, acute kidney injury].

A 65-year-old male was admitted to Peking Union Medical College Hospital. The patient had intermittent fever for 2 months with a maximum body temperature of 39.3 ℃ and elevated serum creatinine levels for 1 week.

A human forebrain organoid model reveals the essential function of GTF2IRD1-TTR-ERK axis for the neurodevelopmental deficits of Williams syndrome.

Williams syndrome (WS; OMIM#194050) is a rare disorder, which is caused by the microdeletion of one copy of 25-27 genes, and WS patients display diverse neuronal deficits. Although remarkable progresses have been achieved, the mechanisms for these distinct deficits are still largely unknown. Here, we have shown that neural progenitor cells (NPCs) in WS forebrain organoids display abnormal proliferation and differentiation capabilities, and synapse formation.

Informed consent and risk communication challenges in antimicrobial clinical trials: a scoping review.

Objectives: Randomised trials for the management of drug-resistant infections are challenging to conduct as target patient populations often lack decision-making capacity, and enrolment windows are typically short. Improving informed consent and risk communication in these trials is especially crucial for protecting patient interests and maximising trial efficiency. This study aimed to understand challenges in risk communication and informed consent in antimicrobial clinical trials.

A multi-modal and multi-stage region of interest-based fusion network convolutional neural network model to differentiate progressive mild cognitive impairment from stable mild cognitive impairment.

Background: Accurately differentiating stable mild cognitive impairment (sMCI) from progressive MCI (pMCI) is clinically relevant, and identification of pMCI is crucial for timely treatment before it evolves into Alzheimer's disease (AD).

Objective: To construct a convolutional neural network (CNN) model to differentiate pMCI from sMCI integrating features from structural magnetic resonance imaging (sMRI) and positron emission tomography (PET) images.

Methods: We proposed a multi-modal and multi-stage region of interest (ROI)-based fusion network (m2ROI-FN) CNN model to differentiate pMCI from sMCI, adopting a multi-stage fusion strategy to integrate deep semantic features and multiple morphological metrics derived from ROIs of sMRI and PET images.

Aristolochic acid-induced dyslipidemia and hepatotoxicity: The potential role of FXR and AHR receptors.

Aristolochic acids (AAs) represent a class of nitrophenanthrene carboxylic acids naturally existing or accidentally mixed in herbal medicines or crops, which have long been recognized for causing nephropathy. Recently, the linkage between AAs and liver injury has become a concern; however, the current understanding of the mechanism or mode of action (MOA) is limited. In the present study, we investigated nuclear receptor-mediated MOA associated with AAs-induced liver injury including dyslipidemia and hepatotoxicity.

Evaluating the Governance Ability of Urban Public Health Using EM-AHP-TOPSIS Method: A Case Study in China.

Background: Evaluating the governance capacity of urban public health is the key to improving the level of urban public health. We aimed to evaluate the governance ability of urban public health.

Methods: An index system of governance ability of urban public health was established.

Color detection of printing based on improved superpixel segmentation algorithm.

We propose an improved superpixel segmentation algorithm based on visual saliency and color entropy for online color detection in printed products. This method addresses the issues of low accuracy and slow speed in detecting color deviations in print quality control. The improved superpixel segmentation algorithm consists of three main steps: Firstly, simulating human visual perception to obtain visually salient regions of the image, thereby achieving region-based superpixel segmentation.

Therapeutic effects of natural compounds against diabetic complications via targeted modulation of ferroptosis.

Diabetes mellitus, a chronic metabolic disorder, can result in serious tissue and organ damage due to long-term metabolic dysfunction, leading to various complications. Therefore, exploring the pathogenesis of diabetic complications and developing effective prevention and treatment drugs is crucial. The role of ferroptosis in diabetic complications has emerged as a significant area of research in recent years.

FGF21 Ameliorates Fibroblasts Activation and Systemic Sclerosis by Inhibiting CK2α/GLI2 Signaling Axis.

Systemic sclerosis is a typical fibrotic disease of unknown etiology that is characterized by abnormal fibroblast activation and excessive deposition of extracellular matrix. Unfortunately, effective therapeutic approaches are lacking. FGF21 plays a key role in mediating a variety of biological activities.

Meta-analysis of Face Perception in Schizophrenia Spectrum Disorders: Evidence for Differential Impairment in Emotion Face Perception.

Background And Hypothesis: Schizophrenia spectrum disorders (SSD) are associated with face perception impairments. It is unclear whether impairments are equal across aspects of face perception or larger-indicating a differential impairment-for perceiving emotions relative to other characteristics (eg, identity, age). While many studies have attempted to compare emotion and non-emotion face perception in SSD, they have varied in design and produced conflicting findings.

Integrating PointNet-Based Model and Machine Learning Algorithms for Classification of Rupture Status of IAs.

Background: The rupture of intracranial aneurysms (IAs) would result in subarachnoid hemorrhage with high mortality and disability. Predicting the risk of IAs rupture remains a challenge.

Methods: This paper proposed an effective method for classifying IAs rupture status by integrating a PointNet-based model and machine learning algorithms.

Characterization techniques for tobacco and its derivatives: a systematic review.

Biomass and its derivatives have broad applications in the fields of bio-catalysis, energy storage, environmental remediation. The structure and components of biomass, which are vital parameters affecting corresponding performances of derived products, need to be fully understood for further regulating the biomass and its derivatives. Herein, tobacco is taken as an example of biomass to introduce the typical characterization techniques in unraveling the structural information, chemical components, and properties of biomass and its derivatives.

Bioactive sucralfate-based microneedles promote wound healing through reprogramming macrophages and protecting endogenous growth factors.

Impaired wound healing due to insufficient cell proliferation and angiogenesis is a significant physical and psychological burden to patients worldwide. Therapeutic delivery of exogenous growth factors (GFs) at high doses for wound repair is non-ideal as GFs have poor stability in proteolytic wound environments. Here, we present a two-stage strategy using bioactive sucralfate-based microneedle (SUC-MN) for delivering interleukin-4 (IL-4) to accelerate wound healing.

LTβR-RelB signaling in intestinal epithelial cells protects from chemotherapy-induced mucosal damage.

The intricate immune mechanisms governing mucosal healing following intestinal damage induced by cytotoxic drugs remain poorly understood. The goal of this study was to investigate the role of lymphotoxin beta receptor (LTβR) signaling in chemotherapy-induced intestinal damage. LTβR deficient mice exhibited heightened body weight loss, exacerbated intestinal pathology, increased proinflammatory cytokine expression, reduced IL-22 expression, and proliferation of intestinal epithelial cells following methotrexate (MTX) treatment.

Virtual reality treatment could reduce anxiety for women undergoing cesarean section with spinal anesthesia: a randomized controlled trial.

Purpose: Cesarean section may result in adverse psychosocial and behavioral outcomes because women put considerable emphasis on the process of birth. Virtual reality treatment has been shown by many studies to reduce anxiety and improve patient satisfaction. Therefore, we designed a randomized controlled trial to investigate whether the application of virtual reality technology during cesarean section can reduce maternal anxiety and improve satisfaction.

Factors associated with peroral endoscopic myotomy for achalasia outcomes: systematic review and meta-analysis.

Background: Peroral endoscopic myotomy (POEM) is an emerging effective treatment for achalasia. However, a significant proportion of patients do not respond well to the treatment. After over a decade of clinical practice, potential risk factors associated with POEM failure have been identified.

The Unified Narcissism Scale-Revised: Testing Incremental Validity and Shortening the Measure.

Narcissism has had a long history of conceptual and measurement confusion. In this paper, we aimed to assess the incremental and external validity of the Unified Narcissism Scale-Revised (UNS-R), and to determine a prototype short form of the measure that is invariant across cultures. In Study 1, we constructed a 15-item short form prototype that was scalar invariant across four countries (United States, China, Sri Lanka, and Australia).

Targeting LRP6: A new strategy for cancer therapy.

Targeting specific molecular drivers of tumor growth is a key approach in cancer therapy. Among these targets, the low-density lipoprotein receptor-related protein 6 (LRP6), a vital component of the Wnt signaling pathway, has emerged as an intriguing candidate. As a cell-surface receptor and vital co-receptor, LRP6 is frequently overexpressed in various cancer types, implicating its pivotal role in driving tumor progression.