Publications by authors named "Shota Yonezawa"

The present study aimed to identify crucial tipping points during neuronal development in a post-mitotic state using Raman spectroscopy and a dynamic network biomarker (DNB) analysis. A DNB analysis is a promising method to detect early signal during state transition. We previously developed an in vitro model that mimics neuronal development.

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Multiple myeloma (MM) is a cancer of plasma cells. Normal (NL) cells are considered to pass through a precancerous state, such as monoclonal gammopathy of undetermined significance (MGUS), before transitioning to MM. In the present study, we acquired Raman spectra at three stages-834 NL, 711 MGUS, and 970 MM spectra-and applied the dynamical network biomarker (DNB) theory to these spectra.

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Article Synopsis
  • Raman spectroscopy provides non-destructive, real-time analysis of living cells and tissues by measuring scattered light, offering insights into their molecular structure and composition.
  • The technique produces a "molecular fingerprint" that helps link specific cellular and tissue compositions to various disease states, aiding in the early detection of diseases.
  • By identifying predisease conditions—when a healthy state shifts toward disease—Raman spectroscopy could play a vital role in preventing disease onset through insights into biological processes and molecular dynamics.
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The dynamical network biomarker (DNB) theory detects the early warning signals of state transitions utilizing fluctuations in and correlations between variables in complex systems. Although the DNB theory has been applied to gene expression in several diseases, destructive testing by microarrays is a critical issue. Therefore, other biological information obtained by non-destructive testing is desirable; one such piece of information is Raman spectra measured by Raman spectroscopy.

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